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Exploring Chromodomain Genes in the Fungus Fusarium graminearum Through Targeted Genetic Manipulation

Creator:: Peters, Alec
Abstract:: DNA segments in the fungus Fusarium graminearum were identified as possible genes encoding for chromodomain proteins (CDPs), and targeted for genetic manipulation. Targeted single-gene deletion strains, as wells as GFP-S tag fusion strains, were generated from wildtype F. graminearum protoplasts, and verified strains were screened for phenotypic differences, such as...
Resource Type:: Honors College Thesis
Full Text:: Manipulation Abstract approved: Michael Freitag DNA segments in the

Exploring Chromodomain Genes in the Fungus Fusarium graminearum Through Targeted Genetic Manipulation

Creator:: Peters, Alec J.
Abstract:: DNA segments in the fungus Fusarium graminearum were identified as possible genes encoding for chromodomain proteins (CDPs), and targeted for genetic manipulation. Targeted single-gene deletion strains, as wells as GFP-S tag fusion strains, were generated from wildtype F. graminearum protoplasts, and verified strains were screened for phenotypic differences, such as...
Resource Type:: Presentation and Honors College Thesis

The role of the putative transcription factor DIS10 in PRC2-mediated gene silencing

Creator:: Jackson, Kendra K.
Abstract:: PRC2 (Polycomb Repressive Complex 2) mediated gene silencing plays an important role in cell differentiation, stem cell plasticity, and tumor suppression. In fungi, it is also involved in pathogenicity. The catalytic subunit, KMT6, trimethylates lysine 27 on histone h3 (H3K27me3) at loci targeted for silencing. In animals, PRC1 and PRC2...
Resource Type:: Honors College Thesis
Full Text:: :_____________________________________________________ Michael Freitag PRC2 (Polycomb Repressive Complex 2) mediated gene silencing plays an

A Panel of Histone H3 Mutations to Investigate Centromere Maintenance and Gene Silencing

Creator:: Esposito, Madison L.
Abstract:: Modifications of histone tail amino acid residues are necessary for many reactions involving chromatin. These processes can produce transcriptionally active, euchromatic, or inactive, heterochromatic, segments of DNA. The formation and maintenance of facultative heterochromatin is critical for the proper growth and development of many eukaryotes. Euchromatin can be changed into...
Resource Type:: Undergraduate Thesis and Presentation
Full Text:: K56Q K56R K56M K56L K64L Q68A Q68S K79Q K79R K79M K79L K122LE133A R134A F. graminearum will be used

Generation of expression plasmids to study the function of the PRC2 subunit Suz12 in Fusarium graminearum

Creator:: Park, Catherine
Abstract:: The Polycomb Repressive Complex 2 (PRC2) catalyzes genome transcription repression through the methylation of lysine residue 27 in the amino terminal region of histone 3 (H3K27me1/2/3) [2], [7]. PRC2 is made up of three highly conserved core subunits: Kmt6, Eed, and Suz12. Accurate distribution of PRC2 is essential for proper...
Resource Type:: Honors College Thesis
Full Text:: Fusarium graminearum. Abstract approved:_____________________________________________________ Michael

A Panel of Histone H3 Mutations to Investigate Centromere Maintenance and Gene Silencing

Creator:: Geisler, Mark S.
Abstract:: Histones, as part of nucleosomes, are responsible for DNA packaging in chromosomes. They also affect DNA expression by a multitude of post-translational modifications, especially prevalent on the amino terminus of histones. Co-activator protein complexes “write”, “read”, or “erase” histone modifications, and in balance determine which sections of DNA are free...
Resource Type:: Honors College Thesis
Full Text:: . Abstract approved:___________________________________________ Michael Freitag Histones, as

Deletion of gene clusters to identify novel secondary metabolites in Fusarium graminearum

Creator:: Husk, Jacob, Connolly, Lanelle, and Freitag, Michael
Abstract:: CGRB Fall Conference 2015
Resource Type:: Poster
Full Text:: Michael Freitag PmeISwaI SecMet cluster DNA Scheme 3: Capture whole clusters with plasmid marker+ 5

Mutations of CenH3 Domains and their Effect on Chromocenter Localization and Meiosis in Neurospora crassa

Creator:: Ferrer, Sarah Theresa
Abstract:: In recent years, non-coding DNA has received much attention within the scientific community. This attention has not only illuminated the mystery behind functionally important regions like the centromere, but has also brought to light additional important questions regarding centromere mechanisms, inheritance, and cellular recognition by the kinetochore, a protein complex...
Resource Type:: Honors College Thesis
Full Text:: : __________________________________________ Michael Freitag In recent years

Mutation of Polycomb Repressive Complex 2 (PRC2) kmt6 to Probe in vivo Function in Fusarium

Creator:: Josephson, Brian
Abstract:: Polycomb Repressive Complex 2 (PRC2) is a histone methyltransferase that initiates facultative heterochromatin formation by trimethylating histone H3 on lysine 27 (H3K27me3). Mutations within its three primary subunits, KMT6, EED, and SUZ12, result in inherited diseases and sporadic cancers. In the fungus Fusarium graminearum, deletion of the gene encoding the...
Resource Type:: Honors College Thesis
Full Text:: . Abstract approved:_____________________________________________________ Michael Freitag

ScholarsArchive@OSU

Exploring Chromodomain Genes in the Fungus Fusarium graminearum Through Targeted Genetic Manipulation

Exploring Chromodomain Genes in the Fungus Fusarium graminearum Through Targeted Genetic Manipulation

The role of the putative transcription factor DIS10 in PRC2-mediated gene silencing

A Panel of Histone H3 Mutations to Investigate Centromere Maintenance and Gene Silencing

Generation of expression plasmids to study the function of the PRC2 subunit Suz12 in Fusarium graminearum

A Panel of Histone H3 Mutations to Investigate Centromere Maintenance and Gene Silencing

Deletion of gene clusters to identify novel secondary metabolites in Fusarium graminearum

Mutations of CenH3 Domains and their Effect on Chromocenter Localization and Meiosis in Neurospora crassa

Mutation of Polycomb Repressive Complex 2 (PRC2) kmt6 to Probe in vivo Function in Fusarium

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