An oral vaccine was developed for control of vibriosis
in Pacific salmon. The vaccine consists of a lyophilized
sonicate of cells of Vibrio anguillarum, the causative
agent of this disease. The preparation was incorporated
into a ration and fed to salmon in fresh water before the
fish were transported and...
This project was initiated in an attempt to control furunculosis
(Aeromonas salmonicida) in a production hatchery rearing coho salmon.
An oral vaccine was prepared and administered to 520,000
juvenile coho salmon (Oncorhynchus kisutch) at the Siletz River Salmon
Hatchery prior to the onset of a predictable naturally occurring
epizootic of...
Fish diseases and various parameters associated with disease
caused mortality of fish were monitored at the Oregon State University
Marine Science Center and at a private mariculture facility on
Yaquina Bay during a period of five years. Nearly all disease problems
observed were caused by Vibrio anguillarum and Vibrio spp....
The purpose of this project was to study the possibility of controlling
furunculosis in salmonid fish using immunological procedures.
Antiserum with an agglutinating antibody titer of 1:20,480
against Aeromonas salmonicida was produced in a horse. This
hyperimmune serum was used to passively immunize coho salmon,
Oncorhynchus kitsutch, (jacks). However, this...
The purpose of this study was to find what effect temperature
and immunization has on the phagocytic activity of fish leukocytes
against certain disease causing bacteria. Also proposed was the
isolation and characterization of a leukocytolytic factor produced by
one of these bacteria, Aeromonas salmonicida.
Investigations revealed that temperature had...
An additional layer, exterior to the outer membrane cellwall
layer, was found on Aeromonas salmonicida cells from aggregating
strains. Nonaggregating strains were found to lack this layer.
The nonaggregating strains were all avirulent (LD₅₀
greater than
1 X 10
⁸ CFU/fish) while the majority of the aggregating strains
were virulent...
The antigens of eight isolates of Renibacterium
salmoninarum were compared serologically using
immunoelectrophoretic techniques. Seven common antigens
were identified by their electrophoretic mobilities in each
antigenic profile. One of these common antigens, designated
Fr 21-30, was partially purified by ammonium sulfate
precipitation and gel filtration. Cross adsorption analysis
showed Fr...
Unfertilized ova from spring chinook salmon (Oncorhynchus
tshawytscha) were examined for the presence of classical salmonid
inmmunoglobulin. Immunodiffusion techniques in which rabbit anti-salmonid
immunoglobulin was reacted against an ova homogenate
and rabbit anti-ova homogenate was reacted against immune chinook
serum failed to detect classical-type antibodies within these eggs.
A lectin...
Phenol-water and trichloroacetic acid extracts of Vibrio
anguillarum were both antigenic and immunogenic when injected
into coho salmon (Oncorhynchus kisutch). Cross-protection against
virulent Vibrio anguillarum was observed with whole-cell bacterins
and phenol-water extracts prepared from two serologically distinct
groups. Gel diffusion studies indicated that intact somatic antigens
were serotype specific....