Graduate Thesis Or Dissertation
 

Comparison of Renibacterium salmoninarum by antigenic analysis, antimicrobial assay and growth

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  • The antigens of eight isolates of Renibacterium salmoninarum were compared serologically using immunoelectrophoretic techniques. Seven common antigens were identified by their electrophoretic mobilities in each antigenic profile. One of these common antigens, designated Fr 21-30, was partially purified by ammonium sulfate precipitation and gel filtration. Cross adsorption analysis showed Fr 21-30 to be the major surface antigen on R. sal moninarum. This purified antigen was heat stable and its molecular weight was estimated at 57,000 by sodium dodecyl sulfate-polyacrylamide gel electrophoresis. Growth studies and antimicrobial assay indicated cultural similarities among the selected isolates of R. salmoninarum.
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