This study was undertaken to compile a comprehensive
review of the literature and to gain a better understanding
of the host-parasite relationship in corynebacterial kidney
disease of salmonid fishes. An indirect fluorescent antibody
technique was developed which allowed differentiation
of intra- and extracellular bacteria. This technique was
used in combination...
An oral vaccine was developed for control of vibriosis
in Pacific salmon. The vaccine consists of a lyophilized
sonicate of cells of Vibrio anguillarum, the causative
agent of this disease. The preparation was incorporated
into a ration and fed to salmon in fresh water before the
fish were transported and...
The detection and antigenic nature of the causative Corynebacterium
of bacterial kidney disease and chemotherapy of this disease
in juvenile coho salmon (Oncorhynchus kisutch) were examined.
Each of 207 yearling coho salmon collected from a population
undergoing a severe epizootic of bacterial kidney disease were examined
for the presence of...
Efficacious vaccines were developed for the control of Vibrio
anguillarum, the etiological agent of vibriosis in salmonid fish.
These bacterins can be administered either orally or parenterally.
It was determined that both formalin-killed lyophilized whole cells
and wet-packed whole cells of the organism are effective oral immunogens.
Intraperitoneal injection of...
Twenty-two isolates of Flexibacter columnaris were
collected from different species of fish from a wide
geographic range. Shieh medium was selected and modified
to provide satisfactory growth of F. columnaris strains.
Although there were three phenotypic variations in colony
morphology among the strains observed, the environmental
and biochemical characteristics as...
Immunity in juvenile coho salmon (Oncorhynchus kisutch) was
evaluated after immunization against vibriosis by parenteral, oral or
immersion methods. Relative levels of immunity were determined by
protection of fish from experimental water-borne challenges with
Vibrio anguillarum and by serum agglutination titers. Coho salmon
eggs were exposed to bacterin during artificial...
The Boivin agent from Vibrio anguillarum was extracted with the use of 0.25M trichloroacetic acid (TCA). Chemical analysis revealed that the endotoxin contained approximately 35.1% protein, 18.1% carbohydrate, 39.3% lipid, 1.8% glucosamine and evidence of ethanolamine. Sixteen different amino acids made up the protein. The aliphatic amino acid concentration was...
Phenol-water and trichloroacetic acid extracts of Vibrio
anguillarum were both antigenic and immunogenic when injected
into coho salmon (Oncorhynchus kisutch). Cross-protection against
virulent Vibrio anguillarum was observed with whole-cell bacterins
and phenol-water extracts prepared from two serologically distinct
groups. Gel diffusion studies indicated that intact somatic antigens
were serotype specific....
Fish diseases and various parameters associated with disease
caused mortality of fish were monitored at the Oregon State University
Marine Science Center and at a private mariculture facility on
Yaquina Bay during a period of five years. Nearly all disease problems
observed were caused by Vibrio anguillarum and Vibrio spp....