The effect of pH and ionic strength on the equilibrium adsorptive
behavior of β-lactoglobulin onto hydrophobic and hydrophilic
silicon surfaces was studied using ellipsometry. Plots of amount
adsorbed (μg protein/cm²) as a function of protein concentration
(mg/ml) exhibited attainment of plateau values beyond a protein
concentration of 0.250 mg/ml. At...
Ellipsometry was used to study the effects of surface energetics
and temperature on the equilibrium adsorptive behavior exhibited by
β-lactoglobulin. β-Lactoglobulin isotherms at 25, 37, and 55°C were
constructed for this purpose. The surfaces of acrylic, polycarbonate,
polyester, glass, and #304 stainless steel were contacted with protein
solutions of varying...
Nisin is an antibacterial peptide, which when adsorbed
on a surface can inhibit bacterial adhesion and viability.
The ability of noncovalently immobilized nisin to withstand
exchange by the milk proteins bovine serum albumin, β-lactoglobulin, α-lactalbumin, and β-casein on surfaces that
had been silanized with dichlorodiethylsilane to exhibit
high and low...
Surface tension kinetics exhibited by selected stability mutants of T4 lysozyme at the air-water interface were monitored with DuNoüy tensiometry. Mutant lysozymes were produced by substitution of isoleucine at position 3 with cysteine, leucine, tryptophan and glycine. Each substitution resulted in an altered structural stability quantified by a change in...
Circular dichroism (CD) spectra were obtained for bacteriophage T4 lysozyme and three of its mutants in the presence and absence of colloidal silica nanoparticles. Mutant lysozymes were produced by substitution of the isoleucine at position 3 with tryptophan, cysteine and leucine. Each substitution resulted in an altered structural stability, quantified...
In this study the enzymatic activity of adsorbed Thermomonospora fusca E₅ and Trichoderma reesei CBHI cellulases were investigated using fluorescence techniques. In particular, cellulases were allowed to contact hydrophobic polystyrene surfaces under conditions of different solution concentrations, and adsorption times. Each of these variables is known to have a potential...
β-lactoglobulin (β-Lg), bovine serum albumin (BSA), α-lactalbumin
(α-Lac), and β-casein were adsorbed onto
silanized silica surfaces of low and high hydrophobicity
for 8 h, and β-Lg and BSA for 1 h. The surfaces were
incubated in buffer for 0, 5, 10, or 15 h and then contacted
with Listeria monocytogenes...
Interactions between proteins and contact surfaces can have
important implications in the food industry. Such interactions
contribute to the course of fouling of membrane surfaces and they
appear to mediate bacterial and spore adhesion to some degree as
well. In addition to protein and solution properties, interfacial
behavior is strongly...
Advancing contact angles formed by water and aqueous ethanol solutions were
measured on both bare surfaces and film-covered surfaces of acrylic, glass, hydrophilic
and hydrophobic silicon, polycarbonate, polyester, and stainless steel. Each bare surface
was initially characterized with respect to its hydrophilic - hydrophobic balance. Both
protein films and milk...
The adsorption kinetics exhibited by selected charge mutants of T4 lysozyme at
silanized silica surfaces were monitored with in situ ellipsometry. Mutant lysozymes were
produced by substitution of lysine (Lys) with glutamic acid (Glu). Each substitution
resulted in a decrease in the net charge of the protein by 2 units....
Adsorption kinetic data recorded for α-lactalbumin, β-casein, β-lactoglobulin, bovine serum albumin and lysozyme at silianized silica surfaces of low and high hydrophobicity, along with a simple model for adsorption and surfactant-mediated elution of protein, were used to analyze the removal of each protein by sodium dodecylsulfate (SDS) and dodecyltrimethylammonium bromide...