Index Catalog // ScholarsArchive@OSU

Structural stability effects on adsorption of bacteriophage T4 lysozyme to colloidal silica

Creator:: Tian, Minghua
Abstract:: Circular dichroism (CD) spectra were obtained for bacteriophage T4 lysozyme and three of its mutants in the presence and absence of colloidal silica nanoparticles. Mutant lysozymes were produced by substitution of the isoleucine at position 3 with tryptophan, cysteine and leucine. Each substitution resulted in an altered structural stability, quantified...
Resource Type:: Masters Thesis
Full Text:: Adsorption of Bacteriophage T4 Lysozyme to Colloidal Silica Abstract approved by: Circular dichroism (CD

Molecular origins of peptide entrapment in polyethylene oxide layers

Creator:: Wu, Xiangming
Abstract:: A more quantitative understanding of peptide entrapment and elution from otherwise protein-repellent polyethylene oxide (PEO) brush layers will provide direction for development of new strategies for drug storage and delivery. Here we describe criteria for peptide integration and structural change within the PEO brush, and discuss the reversibility of peptide...
Resource Type:: Dissertation
Full Text:: , circular dichroism (CD) was used to record the adsorption and conformational changes of (amphiphilic

Binding of bacterial lipopolysaccharide by the cationic amphiphilic peptide WLBU2 at interfaces

Creator:: Ryder, Matthew P.
Abstract:: Passage of blood through a sorbent device for removal of bacteria and endotoxin by specific binding with immobilized, membrane-active, bactericidal peptides holds promise for treating severe blood infections. Peptide insertion in the target membrane and stable binding is desirable, while membrane disruption and release of degradation products to the circulating...
Resource Type:: Dissertation
Full Text:: primarily through his work and analysis of Circular Dichroism (CD). Julie Auxier performed ELISA studies

Nisin adsorption and function at hydrophobic surfaces coated with the poly[ethylene oxide]-poly[propylene oxide]-poly[ethylene oxide] triblock surfactant Pluronic® F108

Creator:: Tai, Yuan-Ching
Abstract:: The adsorption and elution of the antimicrobial peptide nisin at hydrophobic, silanized silica surfaces coated with the poly[ethylene oxide]-poly[propylene oxide]- poly[ethylene oxide] surfactant Pluronic® F108 was measured in situ, with ellipsometry. While such layers are known to inhibit protein adsorption, nisin was observed to adsorb in multilayer quantities, to an...
Resource Type:: Dissertation
Full Text:: - coated substrates was most pronounced in the presence of blood proteins. Circular dichroism

Interaction of recombinant factor VIII and the nonionic surfactant Tween 80 at interfaces

Creator:: Joshi, Omkar
Abstract:: The role of the nonionic surfactant Tween 80 on the behavior of the therapeutic recombinant protein Factor VIII (rFVIII) was investigated at solid/liquid and air/water interfaces. In order to provide a model system to compare results obtained for the complicated rFVIII-Tween system, a well-characterized globular protein lysozyme was used. The...
Resource Type:: Dissertation
Full Text:: secondary structure The circular dichroism spectra of a protein contain information about its secondary

Synthesis and evaluation of PEO-coated materials for microchannel-based hemodialysis

Creator:: Heintz, Keely
Abstract:: The marked increase in surface-to-volume ratio associated with microscale devices for hemodialysis leads to problems with hemocompatibility and blood flow distribution that are more challenging to manage than those encountered at the conventional scale. In this work, stable surface modifications with pendant polyethylene oxide (PEO) chains were produced on polycarbonate...
Resource Type:: Masters Thesis
Full Text:: the past 30 years, with no signs of slowing ....................................................... 8

Retention of protein repulsive character and antimicrobial activity of PEO brush layers following nisin entrapment

Creator:: Auxier, Julie A.
Abstract:: Nisin, an amphiphilic, antimicrobial peptide, has been shown to integrate into the hydrophobic inner region of poly(ethylene oxide) (PEO) brush layers; however, the presence of integrated nisin may compromise the protein repulsive character of the PEO layer. In particular, the introduction of fibrinogen to nisin-loaded brush layers has been observed...
Resource Type:: Masters Thesis
Full Text:: not result in fibrinogen adsorption beyond that expected for a nisin-free PEO layer. No evidence of

Adsorption of synthetic stability mutants of bacteriophage T4 lysozyme at silanized silica surfaces

Creator:: Singla, Brijesh
Resource Type:: Masters Thesis
Full Text:: particles by circular dichroism (CD). The mutants different from wild type by substitution of isoleucine

Fibrinogen adsorption at heparinized silica interfaces : history dependence and adsorption time effects

Creator:: Lee, Hyo Jin
Abstract:: Heparin was modified with adipic diliydrazide and covalently linked to surface-activated silica. Contact angle measurements were made to determine changes in surface at various stages of the derivatization. Xray photoelectron spectroscopy was used to analyze the elemental composition of the surface at each step of immobilizing heparin as well and...
Resource Type:: Masters Thesis
Full Text:: ) was added. The pH of the mixture was maintained at 4.75 for 2 hours or until no further increase in

Surfactant interactions with recombinant factor VIII at the air/water interface

Creator:: Chu, Liping
Abstract:: The surface tension kin'ti4tnd ste'dy state behavior exhibited by mixtures of selected surfactants with a recombinant Factor VIII (rFVIII) were evaluated with automated interfacial tensiometry. In particular, fresh solutions of rFVIII with different concentrations of Tween 80, sodium dodecylsulfate (SDS), or dodecyltrimethylammonium bromide (DTAB) were prepared and their surface tension...
Resource Type:: Masters Thesis
Full Text:: lag time of protein adsorption, during which the surface pressure is 10 about zero, there is no

ScholarsArchive@OSU

Structural stability effects on adsorption of bacteriophage T4 lysozyme to colloidal silica

Molecular origins of peptide entrapment in polyethylene oxide layers

Binding of bacterial lipopolysaccharide by the cationic amphiphilic peptide WLBU2 at interfaces

Nisin adsorption and function at hydrophobic surfaces coated with the poly[ethylene oxide]-poly[propylene oxide]-poly[ethylene oxide] triblock surfactant Pluronic® F108

Interaction of recombinant factor VIII and the nonionic surfactant Tween 80 at interfaces

Synthesis and evaluation of PEO-coated materials for microchannel-based hemodialysis

Retention of protein repulsive character and antimicrobial activity of PEO brush layers following nisin entrapment

Adsorption of synthetic stability mutants of bacteriophage T4 lysozyme at silanized silica surfaces

Fibrinogen adsorption at heparinized silica interfaces : history dependence and adsorption time effects

Surfactant interactions with recombinant factor VIII at the air/water interface

Limit your search

Academic Affiliation

Advisor

Commencement Year

Committee Member

Creator

Date

Decade

Degree Field

Degree Level

Degree Name

File Format

Language

License

Non-Academic Affiliation

Peer Reviewed

Resource Type

Rights Statement

Subject

Search Constraints

Search Results

Limit your search