Index Catalog // ScholarsArchive@OSU

Synthesis and function of bioactive, block copolymer surfactant constructs as relevant to the preparation of anticoagulant and antibacterial medical implant surfaces.

Creator:: Joshi, Pranav R.
Abstract:: Nisin, an antibacterial peptide proven to be an effective inhibitor of Gram-positive bacteria, was incorporated into novel block copolymer constructs and tested for retained antibacterial activity. Covalent coupling was achieved by chemical modification of the N-terminal isoleucine to introduce a thiol group. Thiolated nisin derivatives were then linked to poly[ethylene...
Resource Type:: Dissertation
Full Text:: appreciated. I sincerely appreciate the microbiology tips and hints provided by Dr. Michelle Bothwell and

Nisin adsorption and function at hydrophobic surfaces coated with the poly[ethylene oxide]-poly[propylene oxide]-poly[ethylene oxide] triblock surfactant Pluronic® F108

Creator:: Tai, Yuan-Ching
Abstract:: The adsorption and elution of the antimicrobial peptide nisin at hydrophobic, silanized silica surfaces coated with the poly[ethylene oxide]-poly[propylene oxide]- poly[ethylene oxide] surfactant Pluronic® F108 was measured in situ, with ellipsometry. While such layers are known to inhibit protein adsorption, nisin was observed to adsorb in multilayer quantities, to an...
Resource Type:: Dissertation
Full Text:: . I thank Dr. Michelle Bothwell and Dr. Christine Kelly of the Department of Chemical Engineering

Functionalization of Surfaces with Nisin in a Polyethylene Oxide Brush Layer

Creator:: Ryder, Matthew P.
Abstract:: Infections in hospitals account for over 100,000 deaths per year. These infections occur at the hospital from complications following bacterial adhesion to intravenous catheters, coronary stents and other implanted devices. Another common problem is protein adsorption to the surface of the device and subsequent blood clotting. Methods for combating these...
Resource Type:: Honors College Thesis
Full Text:: incredible and genuine people, both staff and student, including Dr. Michelle Bothwell, Dr. Christine Kelly

Nisin adsorption to PEO-PPO-PEO tri-block copolymer layers and its resistance to elution by fibrinogen

Creator:: Ryder, Matthew P.
Abstract:: While hydrophobic surfaces coated with the poly[ethylene oxide]-poly[propylene oxide]-poly[ethylene oxide] (PEO-PPO-PEO) surfactant Pluronic® F108 are highly resistant to plasma protein adsorption, the antimicrobial peptide nisin has been observed to adsorb in multilayer quantities at such surfaces, and the PEO chains themselves suggested to inhibit nisin exchange by blood proteins. But...
Resource Type:: Masters Thesis
Full Text:: . Secondly, to Dr. Michelle Bothwel, who was my advisor as an undergraduate and without her support, I

Molecular Origins of Recombinant Factor VIII (rFVIII)

Creator:: Tadehara, Katherine M.
Abstract:: The capability of Pluronic® F68 to stabilize human recombinant Factor VIII in solution was assessed using zeta potential measurements. Hemophilia A is a genetic disorder in which the body is missing Factor VIII (FVIII) from the blood coagulation cascade. The pharmaceutical industry developed recombinant Factor VIII (rFVIII), the largest cloned...
Resource Type:: Honors College Thesis
Full Text:: Katherine M. Tadehara A PROJECT Submitted to Oregon State University University

Fibrinogen adsorption at heparinized silica interfaces : history dependence and adsorption time effects

Creator:: Lee, Hyo Jin
Abstract:: Heparin was modified with adipic diliydrazide and covalently linked to surface-activated silica. Contact angle measurements were made to determine changes in surface at various stages of the derivatization. Xray photoelectron spectroscopy was used to analyze the elemental composition of the surface at each step of immobilizing heparin as well and...
Resource Type:: Masters Thesis
Full Text:: we have a chance to work together again in the future. I thank Dr. Michelle Bothwell and Dr. Karyn

Fibrinogen adsorption at heparinized silica interfaces : history dependence and protein concentration effects

Creator:: Joshi, Omkar
Abstract:: Adipic dihydrazide-modified heparin was prepared and subsequently immobilized on surface-activated silica wafers. X-ray photoelectron spectroscopy (XPS) was used to quantify the elemental composition of the silica surface at each stage of activation and results showed that successful immobilization had taken place. Contact angle measurements were made to verify the high...
Resource Type:: Masters Thesis
Full Text:: wholeheartedly say that I worked ''with" Dr. McGuire, not just "under" Dr. McGuire. I thank Dr. Michelle

Surfactant interactions with recombinant factor VIII at the air/water interface

Creator:: Chu, Liping
Abstract:: The surface tension kin'ti4tnd ste'dy state behavior exhibited by mixtures of selected surfactants with a recombinant Factor VIII (rFVIII) were evaluated with automated interfacial tensiometry. In particular, fresh solutions of rFVIII with different concentrations of Tween 80, sodium dodecylsulfate (SDS), or dodecyltrimethylammonium bromide (DTAB) were prepared and their surface tension...
Resource Type:: Masters Thesis
Full Text:: parents. I am grateful to Dr. Michelle Bothwell, she gave me big support to my experiments. Also, thank

Interaction of recombinant factor VIII and the nonionic surfactant Tween 80 at interfaces

Creator:: Joshi, Omkar
Abstract:: The role of the nonionic surfactant Tween 80 on the behavior of the therapeutic recombinant protein Factor VIII (rFVIII) was investigated at solid/liquid and air/water interfaces. In order to provide a model system to compare results obtained for the complicated rFVIII-Tween system, a well-characterized globular protein lysozyme was used. The...
Resource Type:: Dissertation
Full Text:: ensured that I could concentrate on my Ph.D. work while I was in Berkeley. I thank Drs. Michelle Bothwell

Quantifying nisin adsorption behavior at pendant polyethylene oxide brush layers

Creator:: Dill, Justen K.
Abstract:: A more quantitative understanding of peptide loading and release from polyethylene oxide (PEO) brush layers will provide direction for development of new strategies for drug storage and delivery. The antimicrobial peptide nisin shows potent activity against Gram-positive bacteria including the most prevalent implant-associated pathogens, its mechanism of action minimizes the...
Resource Type:: Masters Thesis
Full Text:: have done this without him. I would also like to thank my undergraduate advisor, Dr. Michelle

ScholarsArchive@OSU

Synthesis and function of bioactive, block copolymer surfactant constructs as relevant to the preparation of anticoagulant and antibacterial medical implant surfaces.

Nisin adsorption and function at hydrophobic surfaces coated with the poly[ethylene oxide]-poly[propylene oxide]-poly[ethylene oxide] triblock surfactant Pluronic® F108

Functionalization of Surfaces with Nisin in a Polyethylene Oxide Brush Layer

Nisin adsorption to PEO-PPO-PEO tri-block copolymer layers and its resistance to elution by fibrinogen

Molecular Origins of Recombinant Factor VIII (rFVIII)

Fibrinogen adsorption at heparinized silica interfaces : history dependence and adsorption time effects

Fibrinogen adsorption at heparinized silica interfaces : history dependence and protein concentration effects

Surfactant interactions with recombinant factor VIII at the air/water interface

Interaction of recombinant factor VIII and the nonionic surfactant Tween 80 at interfaces

Quantifying nisin adsorption behavior at pendant polyethylene oxide brush layers

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