Index Catalog // ScholarsArchive@OSU

Elution of adsorbed proteins at hydrophobic and hydrophilic surfaces by dodecyltrimethylammonium bromide and sodium dodecyls sulfate

Creator:: Vinaraphong, Pravina
Abstract:: Adsorption kinetic data recorded for α-lactalbumin, β-casein, β-lactoglobulin, bovine serum albumin and lysozyme at silianized silica surfaces of low and high hydrophobicity, along with a simple model for adsorption and surfactant-mediated elution of protein, were used to analyze the removal of each protein by sodium dodecylsulfate (SDS) and dodecyltrimethylammonium bromide...
Resource Type:: Masters Thesis
Full Text:: Dodecyls Sulfate Abstract approved: -i—*■*=—i- "t Joseph McGuire Adsorption kinetic data recorded

Adsorption of selected charge mutants of bacteriophage T4 lysozyme at silanized silica surfaces

Creator:: Podhipleux, Nilobon
Abstract:: The adsorption kinetics exhibited by selected charge mutants of T4 lysozyme at silanized silica surfaces were monitored with in situ ellipsometry. Mutant lysozymes were produced by substitution of lysine (Lys) with glutamic acid (Glu). Each substitution resulted in a decrease in the net charge of the protein by 2 units....
Resource Type:: Masters Thesis
Full Text:: B Preparation of broths, buffers, and solutions 63 C Averaged values of kinetic rate constants

Molecular origins of peptide entrapment within polyethylene oxide brush layers

Creator:: Lampi, Marsha C.
Abstract:: Effective surface coatings for tissue-contacting medical devices should prevent both biofilm formation and infection. One method to achieve this aim is protein repellant polyethylene oxide (PEO) brush coatings loaded with antimicrobials. Previous research has demonstrated that the antimicrobial nisin adsorbs to PEO brush layers in multi-layer amounts and suggests that...
Resource Type:: Honors College Thesis
Full Text:: AN ABSTRACT OF THE THESIS OF Marsha C

Synthesis and function of bioactive, block copolymer surfactant constructs as relevant to the preparation of anticoagulant and antibacterial medical implant surfaces.

Creator:: Joshi, Pranav R.
Abstract:: Nisin, an antibacterial peptide proven to be an effective inhibitor of Gram-positive bacteria, was incorporated into novel block copolymer constructs and tested for retained antibacterial activity. Covalent coupling was achieved by chemical modification of the N-terminal isoleucine to introduce a thiol group. Thiolated nisin derivatives were then linked to poly[ethylene...
Resource Type:: Dissertation
Full Text:: : _____________________________________________________________________ Joseph McGuire Nisin, an antibacterial peptide proven to be an effective inhibitor of Gram

Nisin adsorption to PEO-PPO-PEO tri-block copolymer layers and its resistance to elution by fibrinogen

Creator:: Ryder, Matthew P.
Abstract:: While hydrophobic surfaces coated with the poly[ethylene oxide]-poly[propylene oxide]-poly[ethylene oxide] (PEO-PPO-PEO) surfactant Pluronic® F108 are highly resistant to plasma protein adsorption, the antimicrobial peptide nisin has been observed to adsorb in multilayer quantities at such surfaces, and the PEO chains themselves suggested to inhibit nisin exchange by blood proteins. But...
Resource Type:: Masters Thesis
Full Text:: approved: ________________________________________________________________________ Joseph McGuire

Kinetic modeling of the adsorption of structural stability mutants of bacteriophage T4 lysozyme at solid-water interfaces

Creator:: Lee, Woo-Kul
Resource Type:: Dissertation
Full Text:: my major professor, Dr. Joseph McGuire for his support, encourage, and endowing me so many

Retention of protein repulsive character and antimicrobial activity of PEO brush layers following nisin entrapment

Creator:: Auxier, Julie A.
Abstract:: Nisin, an amphiphilic, antimicrobial peptide, has been shown to integrate into the hydrophobic inner region of poly(ethylene oxide) (PEO) brush layers; however, the presence of integrated nisin may compromise the protein repulsive character of the PEO layer. In particular, the introduction of fibrinogen to nisin-loaded brush layers has been observed...
Resource Type:: Masters Thesis
Full Text:: : ___________________________________________________________________________ Joseph McGuire Nisin, an amphiphilic, antimicrobial peptide, has been shown to integrate

Characterization and application of PEO-containing triblock copolymer surfactants

Creator:: Snider, Joshua L.
Abstract:: The action of pendant, polyethylene oxide (PEO) brush layers as nonfouling coatings for biomedical materials is well understood. However progress toward clinical application of stable, high density PEO coatings has historically been impeded by the lack of cost effective, noninvasive methods for their preparation. Triblock polymers featuring a hydrophobic, polypropylene...
Resource Type:: Honors College Thesis
Full Text:: : ______________________________________________________________ Joseph McGuire The action of pendant, polyethylene oxide (PEO) brush layers as nonfouling

Fibrinogen adsorption at heparinized silica interfaces : history dependence and protein concentration effects

Creator:: Joshi, Omkar
Abstract:: Adipic dihydrazide-modified heparin was prepared and subsequently immobilized on surface-activated silica wafers. X-ray photoelectron spectroscopy (XPS) was used to quantify the elemental composition of the silica surface at each stage of activation and results showed that successful immobilization had taken place. Contact angle measurements were made to verify the high...
Resource Type:: Masters Thesis
Full Text:: .................................................... 77 C NMR analysis of adipic dihydrazide-modified heparin

Fibrinogen adsorption at heparinized silica interfaces : history dependence and adsorption time effects

Creator:: Lee, Hyo Jin
Abstract:: Heparin was modified with adipic diliydrazide and covalently linked to surface-activated silica. Contact angle measurements were made to determine changes in surface at various stages of the derivatization. Xray photoelectron spectroscopy was used to analyze the elemental composition of the surface at each step of immobilizing heparin as well and...
Resource Type:: Masters Thesis
Full Text:: ................................. 61 B NMR analysis of modified heparin........................ 64 C Toluidine Blue Assay-Detection

ScholarsArchive@OSU

Elution of adsorbed proteins at hydrophobic and hydrophilic surfaces by dodecyltrimethylammonium bromide and sodium dodecyls sulfate

Adsorption of selected charge mutants of bacteriophage T4 lysozyme at silanized silica surfaces

Molecular origins of peptide entrapment within polyethylene oxide brush layers

Synthesis and function of bioactive, block copolymer surfactant constructs as relevant to the preparation of anticoagulant and antibacterial medical implant surfaces.

Nisin adsorption to PEO-PPO-PEO tri-block copolymer layers and its resistance to elution by fibrinogen

Kinetic modeling of the adsorption of structural stability mutants of bacteriophage T4 lysozyme at solid-water interfaces

Retention of protein repulsive character and antimicrobial activity of PEO brush layers following nisin entrapment

Characterization and application of PEO-containing triblock copolymer surfactants

Fibrinogen adsorption at heparinized silica interfaces : history dependence and protein concentration effects

Fibrinogen adsorption at heparinized silica interfaces : history dependence and adsorption time effects

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