Passage of blood through a sorbent device for removal of bacteria and endotoxin by specific binding with immobilized, membrane-active, bactericidal peptides holds promise for treating severe blood infections. Peptide insertion in the target membrane and stable binding is desirable, while membrane disruption and release of degradation products to the circulating...
A more quantitative understanding of peptide entrapment and elution from otherwise protein-repellent polyethylene oxide (PEO) brush layers will provide direction for development of new strategies for drug storage and delivery. Here we describe criteria for peptide integration and structural change within the PEO brush, and discuss the reversibility of peptide...
Amphiphilic gold nanoparticle surfactants can self-assemble at oil-water interfaces to form stable Pickering emulsions. These nanoparticle surfactants have previously been synthesized by functionalizing gold nanoparticles with thiol terminated polyethylene glycol (PEG-thiol), and subsequently with an alkane-thiol. It is necessary to improve the bio-compatibility of the nanoparticles if they are to...
The action of pendant, polyethylene oxide (PEO) brush layers as nonfouling coatings for
biomedical materials is well understood. However progress toward clinical application of stable, high density PEO coatings has historically been impeded by the lack of cost effective, noninvasive methods for their preparation. Triblock polymers featuring a hydrophobic, polypropylene...
Measurable surface parameters which may be related to the response evoked upon contact with biofluids have been suggested to provide direction for characterization, prediction, and ultimate control of biofluid behavior at interfaces. With respect to fouling of heat exchange equipment by biofluids, such properties may be useful for the development...
The effect of pH and ionic strength on the equilibrium adsorptive
behavior of β-lactoglobulin onto hydrophobic and hydrophilic
silicon surfaces was studied using ellipsometry. Plots of amount
adsorbed (μg protein/cm²) as a function of protein concentration
(mg/ml) exhibited attainment of plateau values beyond a protein
concentration of 0.250 mg/ml. At...
Ellipsometry was used to study the effects of surface energetics
and temperature on the equilibrium adsorptive behavior exhibited by
β-lactoglobulin. β-Lactoglobulin isotherms at 25, 37, and 55°C were
constructed for this purpose. The surfaces of acrylic, polycarbonate,
polyester, glass, and #304 stainless steel were contacted with protein
solutions of varying...
Nisin, an amphiphilic, antimicrobial peptide, has been shown to integrate into the hydrophobic inner region of poly(ethylene oxide) (PEO) brush layers; however, the presence of integrated nisin may compromise the protein repulsive character of the PEO layer. In particular, the introduction of fibrinogen to nisin-loaded brush layers has been observed...