The marine psychrophilic bacterium Vibrio marinus MP-4
possessed a maximum temperature and hydrostatic pressure of
20 C and 425 atm for growth. The effects of temperatures of 21 and
25 C and hydrostatic pressures of 200, 400, 500, and 1,000 atm on
protein, RNA and DNA synthesis by V. marinus...
In this study the relationship between salinity and temperature
changes on microbial growth, enzyme induction and substrate uptake
were investigated. The obligately psychrophilic bacterium, V.
marinus MP-1 was grown for 48 hours at 15 C in a glucose-ammonium
medium (GAM) containing 0.4 M NaCl. Log-phase cells were harvested
and shifted...
Washed cells of Vibrio marinus MP-1 grown at 15 C (organism's
optimum growth temperature) were employed in this study. These
cells were exposed to heat at and above their maximal growth temperature
(20 C) for one hour and tested manometrically at 15 C for their
ability to take up oxygen....
The relationship of temperature and salinity to protein synthesis
was determined for cells of Vibrio marinus, MP-1. Protein synthesis
was measured by the incorporation of radioactive proline into
hot trichloroacetic acid precipitable material. At all salinities protein
synthesis occurred at 15°C and 20°C but not at 25°C. The critical
temperature...
Escherichia coli K12, UC41 (an auxotroph) and UC175 (a wild type)
were used to determine possible relationships between binding, uptake,
and respiration and minimum growth temperature.
Both E. coli strains had a minimum growth temperature of 8.0 C in
glucose minimal medium when cells were previously grown at 15 C....
Some enzymes of the citric acid cycle and glycolytic pathway
in cell-free extracts of Vibrio marinus MP-1 were compared for
thermal lability. After one hour of moderate temperature exposure,
enzymes of both pathways rapidly lost catalytic activity. For all
but one enzyme, 50 percent remaining activity occurred near an
averaged...
Vibrio marinus, strain MP-1, was shown to grow from pH 6.7
to 8.5, with an optimum at 7.3. Growth was inhibited below salinity
10%₀ and above 60%₀ . The upper temperature limit of
growth was 20 C, and growth was very rapid with aeration at 15 C.
Heating of the...
Four-fold partially purified glucose-6-phosphate dehydrogenase
(G-6-PDH) from the obligately psychrophilic marine bacterium
Vibrio marinus was effected by thermally induced leakage of the
enzyme, ammonium sulfate fractionation and column chromatography
on Sephadex G-200 gel.
The effects of temperature, pressure, salinity, and pH were
determined for the various degrees of enzyme purity....