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Filtering by: Creator Park, Byung S. Remove constraint Creator: Park, Byung S. Advisor Parks, Leo W. Remove constraint Advisor: Parks, Leo W.

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  • S-adenosylmethionine : [triangle]²⁴-sterol methyltransferase and the regulation of ergosterol biosynthesis in Saccharomyces cerevisiae

    Creator:
    Bailey, Richard B.
    Abstract:
    The inhibition of S-aden.osylmethionine: Δ²⁴-sterol methyltransferase (EC 2.1.1.41) activity by endogenous cellular components has been studied in vitro. The principal inhibitors were Na⁺ and K⁺; Cs⁺, NH₄⁺ and Li⁺ were also shown to inhibit the reaction. The inhibition by potassium cation has been studied, and we have found that energy...
    Resource Type:
    Dissertation
    Full Text:
    : S-ADENOSYLMETHIONINE: 24-STEROL METHYLTRANS- FERASE AND THE REGULATION OF ERGOSTEROL BIOSYNTHESIS

  • The role of S-adenosylmethionine in the biosynthesis of ergosterol and related sterols in Saccharomyces cerevisiae

    Creator:
    Turner, Jan Ross
    Abstract:
    An enzyme system was prepared from Saccharomyces cerevisiae, strain MCC, which carries out the transfer of the methyl group from S-adenosylmethionine or methionine to the side chain of yeast sterols. The cell-free system has been shown to be incapable of synthesizing ergosterol but carries out the synthesis of two as...
    Resource Type:
    Dissertation
    Full Text:
    The Role of S-Adenosylmethionine in the Biosynthesis of

  • Coordinated regulation of plasma membrane lipids in Saccharomyces cerevisiae

    Creator:
    Low, Christopher S. F.
    Abstract:
    Plasma membranes isolated from a yeast sterol auxotroph (RD5-R) grown on 1,5, and 15 pg m1⁻¹ exogenous concentrations of sterol showed no discontinuity in plots of steady-state fluorescence anisotropy. Liposomes constructed from phospholipid and sterol extracted from RD5-R grown on different sterols indicated exogenously supplied sterol modulated cellular phospholipids such...
    Resource Type:
    Dissertation
    Full Text:
    Saccharomyces cerevisiae AN ABSTRACT OF THE THESIS OF Christopher S. F. Low for the degree of Doctor of

  • The terminal reactions in the biosynthesis of methionine in Saccharomyces cerevisiae

    Creator:
    Pigg, C. JoAnne
    Abstract:
    The terminal reactions in the biosynthetic pathway of methionine in Saccharomyces cerevisiae were investigated in this study. Analyses of a number of methionine auxotrophs for biochemical deficiences established four mutant groups. It was observed from qualitative feeding experiments that some auxotrophs were able to utilize S-adenosylmethionine (AM) and S-adenosyl-homocysteine (AH)...
    Resource Type:
    Dissertation
    Full Text:
    some auxotrophs were able to utilize S- adenosyl- methionine (AM)and S- adenosylhomocysteine (AH) to

  • Terminal methylation reactions in Saccharomyces cerevisiae

    Creator:
    Botsford, James Lawrence
    Abstract:
    A cell-free system for assessing the role of S-adenosyl-methionine in the methylation of homocysteine deriving the methyl group from serine has been developed. No role for S-adenosyl-methionine could be shown in the in vitro reaction The enzyme serine transhydroxymethylase has been studied in crude and partially purified cell extracts. Evidence...
    Resource Type:
    Dissertation
    Full Text:
    system for assessing the role of S- adenosyl- methionine in the methylation of homocysteine deriving

  • Defining roles for sterols in Saccharomyces cerevisiae : characterization of bulk membrane and high specificity sparking functions

    Creator:
    Rodriguez, Russell John
    Abstract:
    The ability of cholestanol to support growth of yeast sterol auxotrophs has been examined. Growth on this stanol was precluded unless minute quantities of ergosterol were available, a phenomenon designated sparking. The low levels of ergosterol were insufficient to support growth alone. The data indicate that cholestanol is fulfilling a...
    Resource Type:
    Dissertation
    Full Text:
    fulfilling a bulk membrane requirement(s) while ergosterol satisfies a high specificity sparking function(s

  • Physiological, biochemical, and genetic studies on the [delta]²⁴ sterol methyltransferase of Saccharomyces cerevisiae

    Creator:
    McCammon, Mark Thomas
    Abstract:
    The role of the yeast S-adenosylmethionine: Δ²⁴-sterol-C-methyltransferase (SCMT) in sterol metabolism was investigated. Structural analogs of S-adenosylhomocysteine were tested for inhibition of the SCMT enzyme. A wide inhibitory range by these compounds was observed, indicating which structural features of the parent compound are important for binding to the enzyme. The...
    Resource Type:
    Dissertation
    Full Text:
    S-adenosylmethionine: A24-sterol-C- methyltransferase (SCMT) in sterol metabolism was investigated

  • Regulation of the terminal reactions for methionine biosynthesis in yeast

    Creator:
    Spence, Kemet Dean
    Abstract:
    Studies were carried out to elucidate the mechanism of enzymatic control of methionine biosynthesis in Saccharomyces cerevisiae. Enzymatic studies demonstrated that, in addition to the methionine activating enzyme, the S-adenosylmethionine:homocysteine transmethylase enzyme was induced during cultivation in the presence of excess methionine. A similar, though reduced effect was observed when...
    Resource Type:
    Dissertation
    Full Text:
    enzyme, the S- adenosylmethionine:homocysteine transmethylase enzyme was induced during cultivation in

  • Biosynthesis of yeast sterols

    Creator:
    Turner, Jan Ross
    Abstract:
    An enzyme system from cell-free yeast homogenates mediating the transfer of the methyl group from S-adenosylmethionine-methyl-C¹⁴ or methionine-methyl-C¹⁴ to C-28 of ergosterol has been studied. The effects of divalent cations, suithydryl inhibitors and reducing substances on the activity of the enzyme system have been investigated. It was established that for...
    Resource Type:
    Masters Thesis
    Full Text:
    OF Jan Ross Turner for the M. S. in Microbiology (Name) (Degree) (Major) Date thesis is

  • The cysteine to homocysteine conversion in Saccharomyces cerevisiae

    Creator:
    Sorsoli, Wayne Aldo
    Abstract:
    The mechanism of transsulfuration and the role of cystathionine in the biosynthesis of methionine in yeast were investigated. Saccharomyces were shown to accumulate cystathionine by use of the ³⁵S labeled compound. Cell-free extracts of the wildtype clone as well as methionine auxotrophs were shown to cleave cystathionine. The enzymatically produced...
    Resource Type:
    Dissertation
    Full Text:
    . Carboxyaminopropyl -S- cysteine (cystathionine) (Figure 1) was suggested as an intermediate in this conversion on