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Characterization of substrate-velocity relationships for the cellulase enzyme complex from Trichoderma viride

Creator:: Liaw, Ean-Tun
Abstract:: The influence of substrate and enzyme concentration on the rate of saccharification of two defined, insoluble, cellulose substrates, Avicel and Solka-Floc, by the cellulase enzyme system of Trichoderma viride has been evaluated. Assays utilized enzyme concentrations ranging from 0.014 to 0.056 filter paper unit per mL and substrate concentrations up...
Resource Type:: Masters Thesis
Full Text:: Complex from Trichoderma viride. Abstract approved: , . _ .. ... rr —.—^=— ^ ^ Michael H

Quantitative polysaccharide analysis of lignocellulosic biomass

Creator:: Fenske, John J.
Abstract:: Lignocellulosic biomass is a potential source of fermentable sugars such as glucose. Enzymatic hydrolysis of cellulose is a viable method of solubilizing the glucose from biomass, but the cellulose fraction of native lignocellulosic material is shielded from enzymatic attack by the lignin-hemicellulose matrix surrounding it. Pretreating lignocellulosic biomass with dilute...
Resource Type:: Masters Thesis
Full Text:: Polysaccharide Analysis of Lignocellulosic Biomass Abstract approved: ,. ^ , Michael H. Penner

Initial velocity analyses of milling effects on rates of enzyme-catalyzed saccharification of native wheat straw

Creator:: Nguyen, Quynh M.
Abstract:: There is considerable interest in developing environmentally friendly processes for the economical conversion of biomass plant material to biofuels, bioproducts and biomaterials. One approach to such a conversion process is to make use of enzymes for the conversion of plant polysaccharides to simple sugars; the simple sugars could then be...
Resource Type:: Masters Thesis
Full Text:: : _____________________________________________________ Michael H. Penner There is considerable interest in developing environmentally

Apparent inhibition of Pacific whiting surimi-associated protease by whey protein concentrate

Creator:: Piyachomkwan, Kuakoon
Abstract:: Surimi is a seafood product which is used to manufacture restructured products such as artificial crab and lobster. Surimi is produced from fish fillets by washing to remove sarcoplasmic proteins and increase the concentration of myofibrillar proteins, and mixing with cryoprotectants. A valuable attribute of surimi is its ability to...
Resource Type:: Masters Thesis
Full Text:: approved : . . . . Michael H. Penner, Ph. D. Surimi is a seafood product which is used to

A filter paper assay for low cellulase activities and the cultivation of Trichoderma reesei on acid whey and sweet whey permeate

Creator:: Nordmark, Tor Soren
Abstract:: The traditional filter paper assay for saccharifying cellulase originally described by M. Mandels et al (1976) has been modified to make possible low activity determinations of Trichoderma cellulases. The enzymatic activity appears to decline during a prolonged incubation period if no precautions have been taken. By means of adding bovine...
Resource Type:: Masters Thesis
Full Text:: Acid Whev and Sweet Whev Permeate Abstract approved: Michael H. Penner The traditional filter

Porosity, surface area and enzymatic saccharification of microcrystalline cellulose

Creator:: Tantasucharit, Usicha
Abstract:: The research described in this thesis was aimed at understanding how particle size, porosity, and enzyme accessible surface area influence the rate of saccharification of microcrystalline cellulose. Microcrystalline cellulose (MCC) is a commonly used substrate for the study of cellulolytic enzymes. MCC preparations of different particle size are commercially available....
Resource Type:: Masters Thesis
Full Text:: yields [ES}=yV i L m J (2.10) By rearranging equation 2.10 Substituting equation 2.2 into

Nonstarch polysaccharide fractions of raw, processed and cooked carrots

Creator:: Kim, Sooyoun
Abstract:: The total and soluble nonstarch polysaccharide (NSP) components of raw, processed (canned and frozen) and home-cooked (boiled) Royal Chantenay carrots have been analyzed. NSP fractions were characterized by separation and summation of the monosaccharides resulting from acid hydrolysis of the parent polysaccharides. Total NSP was primarily composed of glucose, ~37%,...
Resource Type:: Masters Thesis
Full Text:: Polvsaccharide Fractions of Raw, Processed and Cooked Carrots Abstract approved: Michael H. Penner The

In Vitro fermentation of dietary cellulose by human fecal microorganisms

Creator:: Chang, Hung-pi
Abstract:: The purpose of the study was to set up an in vitro model of the colon which would permit the analysis of cellulose fermentation by human colonic microflora. Studies of the degradation of polysaccharides by colonic bacteria may help to explain the observed physiological consequences of consuming dietary fiber common...
Resource Type:: Masters Thesis
Full Text:: approved: . .. _, ^^ ^ - Michael H. Penner The purpose of the study was to set up an

Analytical approach to the quantitative analysis of silicon in plants : its application to plant silica extraction

Creator:: Boone, Carmen E.
Abstract:: The literature available on the silica content of herbaceous biomass, and its relative mobility in leaching/extraction systems, includes studies that have employed several different methods for silica quantification. This makes comparison of the data difficult. The objective of this thesis research was to determine the quantitative relationships between the measured...
Resource Type:: Masters Thesis
Full Text:: to Plant Silica Extraction. Abstract approved: Dr. Michael H. Penner The literature

Selective oxidation of biological matrices for subsequent peroxidase and peroxidase-based quantitative analyses

Creator:: Jiang, Shu
Abstract:: Hydrogen peroxide quantification is of broad interest due to the common use of hydrogen peroxide as an oxidizing agent in industrial processing and laboratory research. Hydrogen peroxide assays are also of general importance for biological studies aimed at understanding the role of in situ generated reactive oxygen species. In the...
Resource Type:: Masters Thesis
Full Text:: Peroxidase-Based Quantitative Analyses Abstract approved: Michael H. Penner Hydrogen peroxide

ScholarsArchive@OSU

Characterization of substrate-velocity relationships for the cellulase enzyme complex from Trichoderma viride

Quantitative polysaccharide analysis of lignocellulosic biomass

Initial velocity analyses of milling effects on rates of enzyme-catalyzed saccharification of native wheat straw

Apparent inhibition of Pacific whiting surimi-associated protease by whey protein concentrate

A filter paper assay for low cellulase activities and the cultivation of Trichoderma reesei on acid whey and sweet whey permeate

Porosity, surface area and enzymatic saccharification of microcrystalline cellulose

Nonstarch polysaccharide fractions of raw, processed and cooked carrots

In Vitro fermentation of dietary cellulose by human fecal microorganisms

Analytical approach to the quantitative analysis of silicon in plants : its application to plant silica extraction

Selective oxidation of biological matrices for subsequent peroxidase and peroxidase-based quantitative analyses

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