Search Constraints

Start Over
Filtering by: Creator Michael, Todd P. Remove constraint Creator: Michael, Todd P. Advisor Penner, Michael H. Remove constraint Advisor: Penner, Michael H.

Search Results

  • Interactions of Trichoderma reesei exo-acting cellulases with p-aminophenyl 1-thio-β-D-cellobioside

    Creator:
    Piyachomkwan, Kuakoon
    Abstract:
    Cellulolytic enzymes capable of efficiently degrading crystalline cellulose are a complex mixture of endo- (endoglucanases) and exo-acting (cellobiohydrolases) enzymes. One approach to separating these enzymes is affinity chromatography. A new ligand, p-aminophenyl l-thio-β-D-cellobioside (APTC), is introduced for this purpose. The property of APTC in affinity chromatography is demonstrated using Trichoderma...
    Resource Type:
    Dissertation
    Full Text:
    : Interaction of Trichoderma reesei Exo- Acting Cellulases With Immobilized p-Aminophenyl 1-Thio-^-D

  • Quantification and reactivity of cellulose reducing ends : implication for cellulose

    Creator:
    Kongruang, Sasithorn
    Abstract:
    The primary purpose of this study was to (1) develop methods for the analysis of and (2) provide information on the chemical nature of reducing ends in typical cellulose substrates used for the study of cellulolytic enzymes. The studies were designed such that values obtained for cellulose substrates were compared...
    Resource Type:
    Dissertation
    Full Text:
    . Abstract approved: Michael H. Penn&John P. Bolte The primary purpose of this study was to (1) develop

  • Immobilized cellooligosaccharides in the study of trichoderma reesei cellobiohydrolases

    Creator:
    Sangseethong, Kunruedee
    Abstract:
    A novel type of model substrates, i.e. immobilized p-aminophenyl-β-D-cellooligosaccharides, was developed and used in the study of exocellulases. The two major cellobiohydrolases from Trichoderma reesei, CBH I and CBH II were used as representative enzymes. p-Aminophenyl derivatives of cellobiose (PAPG₂), cellotriose (PAPG₃), and cellotetraose (PAPG₄) were synthesized from the reaction...
    Resource Type:
    Dissertation
    Full Text:
    : •- -1 - ■ /- \y - Michael H. Penner A novel type of model substrates, i.e. immobilized p

  • The Role of Ascorbic Acid, β-Cyclodextrins, and Alternative Substrates in Dictating Rates of Polyphenol Oxidase-Catalyzed Browning in Potato-Based Reaction Mixtures

    Creator:
    Jiang, Shu
    Abstract:
    Enzymatic browning is a consequence of a series of reactions initiated by polyphenol oxidase (PPO)-catalyzed quinone formation. Unwanted browning negatively affects food quality and lowers consumer acceptability. Thus, there is general interest in strategies to control enzymatic browning. Cyclodextrins are cyclic glucan oligosaccharides that form inclusion complexes with a number...
    Resource Type:
    Dissertation
    Full Text:
    : ________________________________________________ Michael H. Penner Enzymatic browning is a consequence of a series of reactions

  • Preparation and modification of cellooligosaccharides

    Creator:
    Akpinar, Ozlem
    Abstract:
    Cellooligosaccharides are the reaction intermediates produced during the hydrolysis of cellulose to glucose. Hence, they have the same chemical structure as cellulose, just shorter chain lengths. Cellooligosaccharides up to DP eight are soluble in water. The soluble cellooligosaccharides can be used "as is" in the food industry as non-digestible oligosaccharides...
    Resource Type:
    Dissertation
    Full Text:
    and Modification of Cellooligosaccharides Abstract approved: Michael H. Penner

  • Analytical approach to the quantitative analysis of silicon in plants : its application to plant silica extraction

    Creator:
    Boone, Carmen E.
    Abstract:
    The literature available on the silica content of herbaceous biomass, and its relative mobility in leaching/extraction systems, includes studies that have employed several different methods for silica quantification. This makes comparison of the data difficult. The objective of this thesis research was to determine the quantitative relationships between the measured...
    Resource Type:
    Masters Thesis
    Full Text:
    to Plant Silica Extraction. Abstract approved: Dr. Michael H. Penner The literature

  • Separation and detection of cellooligosaccharides on cellulose thin-layer chromatography

    Creator:
    Sookavatana, Narumon
    Abstract:
    Linear oligosacchardies of 1,4 linked β-D-glucopyranose are commonly referred to as cellodextrins (CD) or cellooligosaccharides (CO). They are of interest to those working in disciplines involving cellulose chemistry because they are often used as model substrates for cellulose itself. They are of interest to food scientists and nutritionists because they...
    Resource Type:
    Masters Thesis
    Full Text:
    approved: Michael H. Penner Linear oligosacchardies of 1,4 linked P-D-glucopyranose are commonly

  • Selective oxidation of biological matrices for subsequent peroxidase and peroxidase-based quantitative analyses

    Creator:
    Jiang, Shu
    Abstract:
    Hydrogen peroxide quantification is of broad interest due to the common use of hydrogen peroxide as an oxidizing agent in industrial processing and laboratory research. Hydrogen peroxide assays are also of general importance for biological studies aimed at understanding the role of in situ generated reactive oxygen species. In the...
    Resource Type:
    Masters Thesis
    Full Text:
    Peroxidase-Based Quantitative Analyses Abstract approved: Michael H. Penner Hydrogen peroxide

  • Science and efficacy of mild sodium hydroxide treatments in enzyme-based wheat straw-to-glucose processing

    Creator:
    Sophonputtanaphoca, Supaporn
    Abstract:
    The work described in this dissertation focused on chemistry related to the use of aqueous sodium hydroxide as a treatment in the processing of wheat straw. A major emphasis was the comprehensive evaluation of straw component partitioning due to sodium hydroxide (NaOH) processing. This was evaluated over a range of...
    Resource Type:
    Dissertation
    Full Text:
    -­‐ Based  Wheat  Straw-­‐to-­‐Glucose  Processing     Abstract  approved:   Michael  H

  • Salt and polyelectrolyte affect on food colloid function : polygalacturonic acid and egg albumen case studies

    Creator:
    Chen, Chi-Shen
    Abstract:
    The primary purpose of this study was to try to (1) provide information on the chemical nature, including turbidity formation, of polygalacturonic acid in different concentrations of NaCl solution. (2) use turbidity formation characteristics to develop an assay for determining the activities polygalacturonic acid depolymerases by using a polyelectrolyte. (3)...
    Resource Type:
    Dissertation
    Full Text:
    : ______________________________________________________ Michael. H. Penner The primary purpose of this study was to try to (1) provide

  • Characterization of substrate-velocity relationships for the cellulase enzyme complex from Trichoderma viride

    Creator:
    Liaw, Ean-Tun
    Abstract:
    The influence of substrate and enzyme concentration on the rate of saccharification of two defined, insoluble, cellulose substrates, Avicel and Solka-Floc, by the cellulase enzyme system of Trichoderma viride has been evaluated. Assays utilized enzyme concentrations ranging from 0.014 to 0.056 filter paper unit per mL and substrate concentrations up...
    Resource Type:
    Masters Thesis
    Full Text:
    Complex from Trichoderma viride. Abstract approved: , . _ .. ... rr —.—^=— ^ ^ Michael H

  • Initial velocity analyses of milling effects on rates of enzyme-catalyzed saccharification of native wheat straw

    Creator:
    Nguyen, Quynh M.
    Abstract:
    There is considerable interest in developing environmentally friendly processes for the economical conversion of biomass plant material to biofuels, bioproducts and biomaterials. One approach to such a conversion process is to make use of enzymes for the conversion of plant polysaccharides to simple sugars; the simple sugars could then be...
    Resource Type:
    Masters Thesis
    Full Text:
    : _____________________________________________________ Michael H. Penner There is considerable interest in developing environmentally

  • Cellulase system of Trichoderma reesei QM9414 : a study of its apparent sustrate inhibition

    Creator:
    Huang, Xiaolin
    Resource Type:
    Dissertation
    Full Text:
    Abstract approved by : . Michael H. Penner The apparent substrate inhibition properties of

  • A filter paper assay for low cellulase activities and the cultivation of Trichoderma reesei on acid whey and sweet whey permeate

    Creator:
    Nordmark, Tor Soren
    Abstract:
    The traditional filter paper assay for saccharifying cellulase originally described by M. Mandels et al (1976) has been modified to make possible low activity determinations of Trichoderma cellulases. The enzymatic activity appears to decline during a prolonged incubation period if no precautions have been taken. By means of adding bovine...
    Resource Type:
    Masters Thesis
    Full Text:
    Acid Whev and Sweet Whev Permeate Abstract approved: Michael H. Penner The traditional filter

  • The relative activity of the cellulase enzyme system of Trichoderma reesei with native and modified cellulosic substrates

    Creator:
    Liaw, Ean-Tun
    Resource Type:
    Dissertation
    Full Text:
    Substrates Abstract approved by : Michael H. Penner The relative merit of assays commonly applied to

  • Nonstarch polysaccharide fractions of raw, processed and cooked carrots

    Creator:
    Kim, Sooyoun
    Abstract:
    The total and soluble nonstarch polysaccharide (NSP) components of raw, processed (canned and frozen) and home-cooked (boiled) Royal Chantenay carrots have been analyzed. NSP fractions were characterized by separation and summation of the monosaccharides resulting from acid hydrolysis of the parent polysaccharides. Total NSP was primarily composed of glucose, ~37%,...
    Resource Type:
    Masters Thesis
    Full Text:
    Polvsaccharide Fractions of Raw, Processed and Cooked Carrots Abstract approved: Michael H. Penner The

  • Saccharification and fermentation of lignocellulosic biomass using Trichoderma reesei cellulases and Saccharomyces cerevisiae

    Creator:
    Chung, Yun-Chin
    Abstract:
    The efficiency of cellulose hydrolysis under straight saccharification and simultaneous saccharification and fermentation (SSF) conditions was evaluated using three lignocellulosic materials (switchgrass, cornstover, and poplar), which had been pretreated with dilute sulfuric acid under conditions which optimized xylose concentrations in the prehydrolysate liquid. Yields of glucose, cellobiose and ethanol obtained...
    Resource Type:
    Dissertation
    Full Text:
    cerevisiae Abstract approved by: Michael H. Penner The efficiency of cellulose hydrolysis under

  • In Vitro fermentation of dietary cellulose by human fecal microorganisms

    Creator:
    Chang, Hung-pi
    Abstract:
    The purpose of the study was to set up an in vitro model of the colon which would permit the analysis of cellulose fermentation by human colonic microflora. Studies of the degradation of polysaccharides by colonic bacteria may help to explain the observed physiological consequences of consuming dietary fiber common...
    Resource Type:
    Masters Thesis
    Full Text:
    approved: . .. _, ^^ ^ - Michael H. Penner The purpose of the study was to set up an

  • Conversion of lignocellulosic biomass : analysis of substrate characteristics

    Creator:
    Meunier-Goddik, Lisbeth
    Abstract:
    The objective of this research was to investigate the influence of physicochemical properties of lignocellulosic biomass on the rate and extent of cellulose conversion, i.e. the "hydrolyzability" of the substrate. The initial two parts of this thesis investigated the interactions between various components of pretreated biomass. The relevance of cellulase...
    Resource Type:
    Dissertation
    Full Text:
    : - L-- - , Michael H. Penner The objective of this research was to investigate the

  • Utilization of lignocellulosic polysaccharides

    Creator:
    Fenske, John J.
    Abstract:
    Lignocellulosic biomass represents a vast supply of fermentable carbohydrates and functional aromatic compounds. Conversion of lignocellulosics to ethanol and other useful products would be of widespread economical and environmental benefit. Better understanding of the behavior of different lignicellulosic feedstocks in fermentation protocols as well as catalytic activities involved in lignocellulosic...
    Resource Type:
    Dissertation
    Full Text:
    Lignocellulosic Polysaccharides. Abstract approved: ^ _ r . _ . . . . Michael H. Penner

  • Quantitative polysaccharide analysis of lignocellulosic biomass

    Creator:
    Fenske, John J.
    Abstract:
    Lignocellulosic biomass is a potential source of fermentable sugars such as glucose. Enzymatic hydrolysis of cellulose is a viable method of solubilizing the glucose from biomass, but the cellulose fraction of native lignocellulosic material is shielded from enzymatic attack by the lignin-hemicellulose matrix surrounding it. Pretreating lignocellulosic biomass with dilute...
    Resource Type:
    Masters Thesis
    Full Text:
    Polysaccharide Analysis of Lignocellulosic Biomass Abstract approved: ,. ^ , Michael H. Penner

  • Porosity, surface area and enzymatic saccharification of microcrystalline cellulose

    Creator:
    Tantasucharit, Usicha
    Abstract:
    The research described in this thesis was aimed at understanding how particle size, porosity, and enzyme accessible surface area influence the rate of saccharification of microcrystalline cellulose. Microcrystalline cellulose (MCC) is a commonly used substrate for the study of cellulolytic enzymes. MCC preparations of different particle size are commercially available....
    Resource Type:
    Masters Thesis
    Full Text:
    : .. Michael H. Penner The research described in this thesis was aimed at understanding how particle size

  • Wheat lignin as a functional dietary fiber component

    Creator:
    Junyusen, Tiraporn
    Abstract:
    This dissertation has focused on lignin byproducts that result from the alkali processing of wheat straw. The first study evaluated the application of “standard” lignin quantification methods for summative analyses of alkali-based processes. Mass closure during NaOH processing at 25, 50, and 75°C of wheat straw was evaluated using the...
    Resource Type:
    Dissertation
    Full Text:
    : __________________________________________________________________ Michael H. Penner This dissertation has focused on lignin byproducts that result from the

  • Apparent inhibition of Pacific whiting surimi-associated protease by whey protein concentrate

    Creator:
    Piyachomkwan, Kuakoon
    Abstract:
    Surimi is a seafood product which is used to manufacture restructured products such as artificial crab and lobster. Surimi is produced from fish fillets by washing to remove sarcoplasmic proteins and increase the concentration of myofibrillar proteins, and mixing with cryoprotectants. A valuable attribute of surimi is its ability to...
    Resource Type:
    Masters Thesis
    Full Text:
    approved : . . . . Michael H. Penner, Ph. D. Surimi is a seafood product which is used to

  • Influence of extractives on the chemical analysis of switchgrass

    Creator:
    Tandjo, Djuhartini
    Abstract:
    This thesis summarizes an investigation into the need for removing extractives from herbaceous biomass feedstocks prior to their chemical characterization. Switchgrass (Panicum virgatum), was used in this study as a representative herbaceous biomass feedstock. The influence of extractives on the chemical analysis of switchgrass was done by comparing the composition...
    Resource Type:
    Masters Thesis
    Full Text:
    Michael H. Penner This thesis summarizes an investigation into the need for removing extractives from

  • Digesta passage rates in the rat

    Creator:
    Luick, Bret R.
    Abstract:
    The movements of digestion markers in the gut of the rat were investigated and the results of the investigation are reported in two manuscripts. The objective of the first study was to determine the influence of particle size of dietary wheat bran, chosen as a representative insoluble fiber, on the...
    Resource Type:
    Dissertation
    Full Text:
    Passage Rates in the Rat Abstract approved: , | . Michael H. Penner The movements of digestion

  • Composition and in vitro digestion of barley, oat, and wheat brans

    Creator:
    Gangloff, Mary Beth
    Abstract:
    In the field of nutrition, complex carbohydrates have become increasingly important in the last decade. The fiber fraction of foods has been implicated as modifying the etiology of several chronic diseases and the starch fraction is important with respect to the glycemic index of foods. This study has focused on...
    Resource Type:
    Masters Thesis
    Full Text:
    ;Composition and in vitro Digestion of Barley. Oat, and Wheat Brans Abstract approved: = Michael H. Penner

  • The role of molecular architecture in the hydration properties of brewers’ spent grain

    Creator:
    Yang, Jasmin S.
    Abstract:
    An emerging strategy to reducing food waste is converting food processing byproducts to nutritious and inexpensive food ingredients. A relevant food processing waste stream is brewers’ spent grain (BSG), the primary byproduct of beer production. BSG is a promising candidate as a food ingredient because of its high protein and...
    Resource Type:
    Honors College Thesis
    Full Text:
    :_____________________________________________________ Michael H. Penner An emerging strategy to reducing food waste is converting food

  • The role of solvent extraction in the chemical characterization of corn stover feedstock

    Creator:
    Thammasouk, Khamphet
    Abstract:
    The consequences of extracting com stover feedstock with either 95% ethanol or hot water prior to the chemical analysis of the macrocomponents of that feedstock have been determined. Reports by others have recommended the removal of extraneous substance by solvent extraction prior to chemical analyses (Browning, 1967; TAPPI, 1988). The...
    Resource Type:
    Masters Thesis
    Full Text:
    Michael H. Penner The consequences of extracting com stover feedstock with either 95% ethanol or hot

  • Data Mining in Food Science Research and Technical Functionality of Wheat Straw-Derived Fibers

    Creator:
    Karaarslan Urhan, Tuba
    Abstract:
    This dissertation focused on data mining in food science research and on the technical functional properties of wheat straw-derived biorefinery-byproduct fiber preparations as related to potential applications as food ingredients. This first study evaluated the importance of electronic bibliographic database selection and multiple database usage during the information retrieval phase...
    Resource Type:
    Dissertation
    Full Text:
    : ______________________________________________________ Michael H. Penner This dissertation focused on data mining in food science research and