Index Catalog // ScholarsArchive@OSU

Immobilized cellooligosaccharides in the study of trichoderma reesei cellobiohydrolases

Creator:: Sangseethong, Kunruedee
Abstract:: A novel type of model substrates, i.e. immobilized p-aminophenyl-β-D-cellooligosaccharides, was developed and used in the study of exocellulases. The two major cellobiohydrolases from Trichoderma reesei, CBH I and CBH II were used as representative enzymes. p-Aminophenyl derivatives of cellobiose (PAPG₂), cellotriose (PAPG₃), and cellotetraose (PAPG₄) were synthesized from the reaction...
Resource Type:: Dissertation
Full Text:: APPROVED: ^AAi^^^«^ Major Professor, representing Food Science and Technology i' T ' -ft r

Interactions of Trichoderma reesei exo-acting cellulases with p-aminophenyl 1-thio-β-D-cellobioside

Creator:: Piyachomkwan, Kuakoon
Abstract:: Cellulolytic enzymes capable of efficiently degrading crystalline cellulose are a complex mixture of endo- (endoglucanases) and exo-acting (cellobiohydrolases) enzymes. One approach to separating these enzymes is affinity chromatography. A new ligand, p-aminophenyl l-thio-β-D-cellobioside (APTC), is introduced for this purpose. The property of APTC in affinity chromatography is demonstrated using Trichoderma...
Resource Type:: Dissertation
Full Text:: , representing Food Science and Technology 1^ « —r -r 1 J_ —,- ———* J- 1>- ■ ^-^— ', Header Department of

Preparation and modification of cellooligosaccharides

Creator:: Akpinar, Ozlem
Abstract:: Cellooligosaccharides are the reaction intermediates produced during the hydrolysis of cellulose to glucose. Hence, they have the same chemical structure as cellulose, just shorter chain lengths. Cellooligosaccharides up to DP eight are soluble in water. The soluble cellooligosaccharides can be used "as is" in the food industry as non-digestible oligosaccharides...
Resource Type:: Dissertation
Full Text:: al., 1996; Beguin and Aubert, 2000). 17 Crystalline Amorphous region region R

Conversion of lignocellulosic biomass : analysis of substrate characteristics

Creator:: Meunier-Goddik, Lisbeth
Abstract:: The objective of this research was to investigate the influence of physicochemical properties of lignocellulosic biomass on the rate and extent of cellulose conversion, i.e. the "hydrolyzability" of the substrate. The initial two parts of this thesis investigated the interactions between various components of pretreated biomass. The relevance of cellulase...
Resource Type:: Dissertation
Full Text:: and Bioengineering 36:446-452. Otter, D.E., Munro, P.A., Scott, G.K., and R. Geddes. 1989

Characterization of substrate-velocity relationships for the cellulase enzyme complex from Trichoderma viride

Creator:: Liaw, Ean-Tun
Abstract:: The influence of substrate and enzyme concentration on the rate of saccharification of two defined, insoluble, cellulose substrates, Avicel and Solka-Floc, by the cellulase enzyme system of Trichoderma viride has been evaluated. Assays utilized enzyme concentrations ranging from 0.014 to 0.056 filter paper unit per mL and substrate concentrations up...
Resource Type:: Masters Thesis

Quantification and reactivity of cellulose reducing ends : implication for cellulose

Creator:: Kongruang, Sasithorn
Abstract:: The primary purpose of this study was to (1) develop methods for the analysis of and (2) provide information on the chemical nature of reducing ends in typical cellulose substrates used for the study of cellulolytic enzymes. The studies were designed such that values obtained for cellulose substrates were compared...
Resource Type:: Dissertation
Full Text:: authorizes release of my thesis to any reader upor'requt r - Sasithorn Kongruang, Author Redacted for

Separation and detection of cellooligosaccharides on cellulose thin-layer chromatography

Creator:: Sookavatana, Narumon
Abstract:: Linear oligosacchardies of 1,4 linked β-D-glucopyranose are commonly referred to as cellodextrins (CD) or cellooligosaccharides (CO). They are of interest to those working in disciplines involving cellulose chemistry because they are often used as model substrates for cellulose itself. They are of interest to food scientists and nutritionists because they...
Resource Type:: Masters Thesis
Full Text:: I 1, 2001 APPROVED: £ n r T «—s ^^—— — ^=^ Major Professor, representing Food Science

In Vitro fermentation of dietary cellulose by human fecal microorganisms

Creator:: Chang, Hung-pi
Abstract:: The purpose of the study was to set up an in vitro model of the colon which would permit the analysis of cellulose fermentation by human colonic microflora. Studies of the degradation of polysaccharides by colonic bacteria may help to explain the observed physiological consequences of consuming dietary fiber common...
Resource Type:: Masters Thesis
Full Text:: levels of magnesium and calcium remained within normal limits. In 1981, Bhat(90) reported that addition

Data Mining in Food Science Research and Technical Functionality of Wheat Straw-Derived Fibers

Creator:: Karaarslan Urhan, Tuba
Abstract:: This dissertation focused on data mining in food science research and on the technical functional properties of wheat straw-derived biorefinery-byproduct fiber preparations as related to potential applications as food ingredients. This first study evaluated the importance of electronic bibliographic database selection and multiple database usage during the information retrieval phase...
Resource Type:: Dissertation

The relative activity of the cellulase enzyme system of Trichoderma reesei with native and modified cellulosic substrates

Creator:: Liaw, Ean-Tun
Resource Type:: Dissertation
Full Text:: , 1994 APPROVED _£_£ fc£^ Associate Professor of Food Science and Technology He^of • —r \L —2

ScholarsArchive@OSU

Immobilized cellooligosaccharides in the study of trichoderma reesei cellobiohydrolases

Interactions of Trichoderma reesei exo-acting cellulases with p-aminophenyl 1-thio-β-D-cellobioside

Preparation and modification of cellooligosaccharides

Conversion of lignocellulosic biomass : analysis of substrate characteristics

Characterization of substrate-velocity relationships for the cellulase enzyme complex from Trichoderma viride

Quantification and reactivity of cellulose reducing ends : implication for cellulose

Separation and detection of cellooligosaccharides on cellulose thin-layer chromatography

In Vitro fermentation of dietary cellulose by human fecal microorganisms

Data Mining in Food Science Research and Technical Functionality of Wheat Straw-Derived Fibers

The relative activity of the cellulase enzyme system of Trichoderma reesei with native and modified cellulosic substrates

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