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Filtering by: Creator Olsen, Michael J. Remove constraint Creator: Olsen, Michael J. Advisor Penner, Michael H. Remove constraint Advisor: Penner, Michael H.

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  • Characterization of substrate-velocity relationships for the cellulase enzyme complex from Trichoderma viride

    Creator:
    Liaw, Ean-Tun
    Abstract:
    The influence of substrate and enzyme concentration on the rate of saccharification of two defined, insoluble, cellulose substrates, Avicel and Solka-Floc, by the cellulase enzyme system of Trichoderma viride has been evaluated. Assays utilized enzyme concentrations ranging from 0.014 to 0.056 filter paper unit per mL and substrate concentrations up...
    Resource Type:
    Masters Thesis
    Full Text:
    Complex from Trichoderma viride. Abstract approved: , . _ .. ... rr —.—^=— ^ ^ Michael H

  • Utilization of lignocellulosic polysaccharides

    Creator:
    Fenske, John J.
    Abstract:
    Lignocellulosic biomass represents a vast supply of fermentable carbohydrates and functional aromatic compounds. Conversion of lignocellulosics to ethanol and other useful products would be of widespread economical and environmental benefit. Better understanding of the behavior of different lignicellulosic feedstocks in fermentation protocols as well as catalytic activities involved in lignocellulosic...
    Resource Type:
    Dissertation
    Full Text:
    . John J. Fenske, Author Acknowledgements Professor Michael H. Penner has earned my deepest respect

  • Quantitative polysaccharide analysis of lignocellulosic biomass

    Creator:
    Fenske, John J.
    Abstract:
    Lignocellulosic biomass is a potential source of fermentable sugars such as glucose. Enzymatic hydrolysis of cellulose is a viable method of solubilizing the glucose from biomass, but the cellulose fraction of native lignocellulosic material is shielded from enzymatic attack by the lignin-hemicellulose matrix surrounding it. Pretreating lignocellulosic biomass with dilute...
    Resource Type:
    Masters Thesis
    Full Text:
    Polysaccharide Analysis of Lignocellulosic Biomass Abstract approved: ,. ^ , Michael H. Penner

  • Initial velocity analyses of milling effects on rates of enzyme-catalyzed saccharification of native wheat straw

    Creator:
    Nguyen, Quynh M.
    Abstract:
    There is considerable interest in developing environmentally friendly processes for the economical conversion of biomass plant material to biofuels, bioproducts and biomaterials. One approach to such a conversion process is to make use of enzymes for the conversion of plant polysaccharides to simple sugars; the simple sugars could then be...
    Resource Type:
    Masters Thesis
    Full Text:
    : _____________________________________________________ Michael H. Penner There is considerable interest in developing environmentally

  • The relative activity of the cellulase enzyme system of Trichoderma reesei with native and modified cellulosic substrates

    Creator:
    Liaw, Ean-Tun
    Resource Type:
    Dissertation
    Full Text:
    Substrates Abstract approved by : Michael H. Penner The relative merit of assays commonly applied to

  • Quantification and reactivity of cellulose reducing ends : implication for cellulose

    Creator:
    Kongruang, Sasithorn
    Abstract:
    The primary purpose of this study was to (1) develop methods for the analysis of and (2) provide information on the chemical nature of reducing ends in typical cellulose substrates used for the study of cellulolytic enzymes. The studies were designed such that values obtained for cellulose substrates were compared...
    Resource Type:
    Dissertation
    Full Text:
    . Abstract approved: Michael H. Penn&John P. Bolte The primary purpose of this study was to (1) develop

  • Interactions of Trichoderma reesei exo-acting cellulases with p-aminophenyl 1-thio-β-D-cellobioside

    Creator:
    Piyachomkwan, Kuakoon
    Abstract:
    Cellulolytic enzymes capable of efficiently degrading crystalline cellulose are a complex mixture of endo- (endoglucanases) and exo-acting (cellobiohydrolases) enzymes. One approach to separating these enzymes is affinity chromatography. A new ligand, p-aminophenyl l-thio-β-D-cellobioside (APTC), is introduced for this purpose. The property of APTC in affinity chromatography is demonstrated using Trichoderma...
    Resource Type:
    Dissertation
    Full Text:
    -Cellobioside. Abstract approved: ,__ Michael H. Penner Cellulolytic enzymes capable of efficiently

  • Nonstarch polysaccharide fractions of raw, processed and cooked carrots

    Creator:
    Kim, Sooyoun
    Abstract:
    The total and soluble nonstarch polysaccharide (NSP) components of raw, processed (canned and frozen) and home-cooked (boiled) Royal Chantenay carrots have been analyzed. NSP fractions were characterized by separation and summation of the monosaccharides resulting from acid hydrolysis of the parent polysaccharides. Total NSP was primarily composed of glucose, ~37%,...
    Resource Type:
    Masters Thesis
    Full Text:
    Polvsaccharide Fractions of Raw, Processed and Cooked Carrots Abstract approved: Michael H. Penner The

  • Salt and polyelectrolyte affect on food colloid function : polygalacturonic acid and egg albumen case studies

    Creator:
    Chen, Chi-Shen
    Abstract:
    The primary purpose of this study was to try to (1) provide information on the chemical nature, including turbidity formation, of polygalacturonic acid in different concentrations of NaCl solution. (2) use turbidity formation characteristics to develop an assay for determining the activities polygalacturonic acid depolymerases by using a polyelectrolyte. (3)...
    Resource Type:
    Dissertation
    Full Text:
    : ______________________________________________________ Michael. H. Penner The primary purpose of this study was to try to (1) provide

  • In Vitro fermentation of dietary cellulose by human fecal microorganisms

    Creator:
    Chang, Hung-pi
    Abstract:
    The purpose of the study was to set up an in vitro model of the colon which would permit the analysis of cellulose fermentation by human colonic microflora. Studies of the degradation of polysaccharides by colonic bacteria may help to explain the observed physiological consequences of consuming dietary fiber common...
    Resource Type:
    Masters Thesis
    Full Text:
    approved: . .. _, ^^ ^ - Michael H. Penner The purpose of the study was to set up an