Cellulolytic enzymes capable of efficiently degrading crystalline cellulose are a
complex mixture of endo- (endoglucanases) and exo-acting (cellobiohydrolases)
enzymes. One approach to separating these enzymes is affinity chromatography. A new
ligand, p-aminophenyl l-thio-β-D-cellobioside (APTC), is introduced for this purpose.
The property of APTC in affinity chromatography is demonstrated using Trichoderma...
The purpose of the study was to set up an in vitro model of the colon which
would permit the analysis of cellulose fermentation by human colonic microflora. Studies
of the degradation of polysaccharides by colonic bacteria may help to explain the observed
physiological consequences of consuming dietary fiber common...
A novel type of model substrates, i.e. immobilized p-aminophenyl-β-D-cellooligosaccharides,
was developed and used in the study of exocellulases. The
two major cellobiohydrolases from Trichoderma reesei, CBH I and CBH II were
used as representative enzymes. p-Aminophenyl derivatives of cellobiose (PAPG₂),
cellotriose (PAPG₃), and cellotetraose (PAPG₄) were synthesized from the reaction...
Linear oligosacchardies of 1,4 linked β-D-glucopyranose are commonly referred to as cellodextrins (CD) or cellooligosaccharides (CO). They are of interest to those working in disciplines involving cellulose chemistry because they are often used as model substrates for cellulose itself. They are of interest to food scientists and nutritionists because they...
Cellooligosaccharides are the reaction intermediates produced during the hydrolysis of cellulose to glucose. Hence, they have the same chemical structure as cellulose, just shorter chain lengths. Cellooligosaccharides up to DP eight are soluble in water. The soluble cellooligosaccharides can be used "as is" in the food industry as non-digestible oligosaccharides...
The primary purpose of this study was to try to (1) provide information on the chemical nature, including turbidity formation, of polygalacturonic acid in different concentrations of NaCl solution. (2) use turbidity formation characteristics to develop an assay for determining the activities polygalacturonic acid depolymerases by using a polyelectrolyte. (3)...
There is considerable interest in developing environmentally friendly processes for the economical conversion of biomass plant material to biofuels, bioproducts and biomaterials. One approach to such a conversion process is to make use of enzymes for the conversion of plant polysaccharides to simple sugars; the simple sugars could then be...
The influence of substrate and enzyme concentration on the rate
of saccharification of two defined, insoluble, cellulose substrates,
Avicel and Solka-Floc, by the cellulase enzyme system of Trichoderma
viride has been evaluated. Assays utilized enzyme concentrations
ranging from 0.014 to 0.056 filter paper unit per mL and substrate
concentrations up...
Surimi is a seafood product which is used to manufacture restructured products
such as artificial crab and lobster. Surimi is produced from fish fillets by washing to
remove sarcoplasmic proteins and increase the concentration of myofibrillar proteins, and
mixing with cryoprotectants. A valuable attribute of surimi is its ability to...
The efficiency of cellulose hydrolysis under straight saccharification and simultaneous saccharification and fermentation (SSF) conditions was evaluated using three lignocellulosic materials (switchgrass, cornstover, and poplar), which had been pretreated with dilute sulfuric acid under conditions which optimized xylose concentrations in the prehydrolysate liquid. Yields of glucose, cellobiose and ethanol obtained...