In-vitro plants in slow-growth storage must be routinely evaluated for repropagation
and assessment of viability. It is often difficult to determine plant health by visual
assessment due to variation in growth patterns and plant structure among genera. In
order to evaluate cold-stored germplasm, laboratories need more effective approaches for
viability...
Roots of greenhouse-grown peppermint (Mentha piperita L.) and spearmint (Mentha spicata L.)
plants and in vitro-grown shoot cultures were inoculated with Verticillium dahliae conidial suspensions to
study symptom development, detection, and elimination of the V. dahliae. There were significant
differences in the symptom expression of control and infected shoot cultures...
Improved recovery of cryopreserved meristems and calli from in
vitro currant (Ribes aureum Pursh and R. ciliatum Humb. & Bonpl.) plants
was obtained by two-hour pretreatment with sucrose, proline, abscisic
acid-responsive protein (RABP), and bovine serum albumin (BSA)
solutions. Meristems and calli cold-acclimatized for one week were
immersed in pretreatment...
Bacterial contamination is one of the most important problems
both in plant tissue culture research and commercial laboratories. Early
detection of contaminants saves time, effort, and money. Strawberry
runner explants were screened for contaminants during June to August,
1994 and 1995. Most contaminants found were bacterial rather than
fungal. Bacterial...
Genetic stability of micropropagated and cold-stored Fragaria x anannasa cultivar
'Pocahontas' was evaluated by Random Amplified Polymorphic DNA (RAPD) assay.
Plantlets grown for eight months on M & S medium with 5 μM and 15 μM N6-
benzyladenine (BA) were analyzed. No evidence of mutations was seen in over 200...
Alternating temperature (22°C / -1°C) pretreatments were effective for
increasing cold hardiness and recovery of cryopreserved meristems. Combining low
temperature pretreatment with abscisic acid (ABA) or sucrose treatments reduced the
time required for optimal cold hardiness of meristems. Desiccation tolerance was
directly correlated with increased cold hardiness and recovery of...