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Filtering by: Creator Reed, Cheryl J. Remove constraint Creator: Reed, Cheryl J. Advisor Reed, Barbara M. Remove constraint Advisor: Reed, Barbara M.

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  • Improving in vitro mineral nutrition for diverse red raspberry and Rubus germplasm

    Creator:
    Poothong, Sukalya
    Abstract:
    Red raspberry, Rubus idaeus L., is a valuable crop for the U.S. Pacific Northwest and clonal propagation is required to produce disease-free plants and for germplasm preservation. One challenge of red raspberry micropropagation is the wide variation in growth response among the cultivars. The studies described in this dissertation were...
    Resource Type:
    Dissertation
    Full Text:
    Abstract approved: _____________________________________________________ Barbara M. Reed

  • Detection, identification, and antibiotic treatments of bacterial contaminants from micropropagated strawberries

    Creator:
    Tanprasert, Piyarak
    Abstract:
    Bacterial contamination is one of the most important problems both in plant tissue culture research and commercial laboratories. Early detection of contaminants saves time, effort, and money. Strawberry runner explants were screened for contaminants during June to August, 1994 and 1995. Most contaminants found were bacterial rather than fungal. Bacterial...
    Resource Type:
    Masters Thesis
    Full Text:
    approved: - - ,- -. Barbara M. Reed Bacterial contamination is one of the most important problems

  • Rubus leaf regeneration and micropropagation of virus infected raspberry

    Creator:
    Tsao, Chih-wei V.
    Resource Type:
    Masters Thesis
    Full Text:
    : . _ . . Barbara M. Reed An in vitro regeneration system was developed for leaf explants of Rubus cultivars

  • Improvement of cryopreservation regrowth of in vitro Ribes by pretreatment

    Creator:
    Luo, Jie, 1968-
    Abstract:
    Improved recovery of cryopreserved meristems and calli from in vitro currant (Ribes aureum Pursh and R. ciliatum Humb. & Bonpl.) plants was obtained by two-hour pretreatment with sucrose, proline, abscisic acid-responsive protein (RABP), and bovine serum albumin (BSA) solutions. Meristems and calli cold-acclimatized for one week were immersed in pretreatment...
    Resource Type:
    Masters Thesis
    Full Text:
    Regrowth of In Vitro Ribes bv Pretreatment. Abstract approved: Barbara M. Reed Improved recovery of

  • Nondestructive evaluation of in vitro-stored plants : a comparison of visual and image analysis systems

    Creator:
    Aynalem, Hailu M.
    Abstract:
    In-vitro plants in slow-growth storage must be routinely evaluated for repropagation and assessment of viability. It is often difficult to determine plant health by visual assessment due to variation in growth patterns and plant structure among genera. In order to evaluate cold-stored germplasm, laboratories need more effective approaches for viability...
    Resource Type:
    Masters Thesis
    Full Text:
    : Barbara M. Reed In-vitro plants in slow-growth storage must be routinely evaluated for repropagation

  • Development, detection and elimination of Verticillium dahliae in mint shoot cultures and effects of bacterial cultures and bacterization on the growth and disease development

    Creator:
    Wang, Nan
    Abstract:
    Roots of greenhouse-grown peppermint (Mentha piperita L.) and spearmint (Mentha spicata L.) plants and in vitro-grown shoot cultures were inoculated with Verticillium dahliae conidial suspensions to study symptom development, detection, and elimination of the V. dahliae. There were significant differences in the symptom expression of control and infected shoot cultures...
    Resource Type:
    Masters Thesis
    Full Text:
    Bacterization on the Growth and Disease Development Abstract approved:' Barbara M. Reed Roots of greenhouse

  • Genetic stability of micropropagated and in vitro cold-stored strawberries

    Creator:
    Kumar, Mohan B.
    Abstract:
    Genetic stability of micropropagated and cold-stored Fragaria x anannasa cultivar 'Pocahontas' was evaluated by Random Amplified Polymorphic DNA (RAPD) assay. Plantlets grown for eight months on M & S medium with 5 μM and 15 μM N6- benzyladenine (BA) were analyzed. No evidence of mutations was seen in over 200...
    Resource Type:
    Masters Thesis
    Full Text:
    Micropropagated and In vitro Cold-stored Strawberries. Abstract approved^ _ . , Barbara M. Reed Genetic

  • Fingerprinting and genetic stability of Rubus using molecular markers

    Creator:
    Castillo, Nina Rosa F.
    Abstract:
    Two studies used DNA markers to assess their usefulness in germplasm identification and evaluation of genetic stability in four cryopreserved Rubus accessions that were stored for over 10 years in liquid nitrogen. In the first study, 12 robust Simple Sequence Repeat (SSR) markers were developed from two microsatellite-enriched libraries of...
    Resource Type:
    Masters Thesis
    Full Text:
    Abstract approved: _____________________________________________________ Barbara M. Reed Two

  • Cryopreservation of in vitro-grown apical meristems

    Creator:
    Chang, Yongjian
    Abstract:
    Alternating temperature (22°C / -1°C) pretreatments were effective for increasing cold hardiness and recovery of cryopreserved meristems. Combining low temperature pretreatment with abscisic acid (ABA) or sucrose treatments reduced the time required for optimal cold hardiness of meristems. Desiccation tolerance was directly correlated with increased cold hardiness and recovery of...
    Resource Type:
    Dissertation
    Full Text:
    Vitro-Grown Apical Meristems. Abstract approved^ . . Barbara M. Reed Alternating temperature (220C

  • Micropropagation of Pyrus rootstocks

    Creator:
    Yeo, Dennis
    Abstract:
    The availability of new pear rootstocks could be increased by development of an efficient micropropagation system. Many P. calleryana, P. betulifolia and P. communis rootstocks are resistant to many pear diseases and are graft compatible with leading cultivars. Explains of three potentially useful rootstock selections P. calleryana Decne 'OPR 157',...
    Resource Type:
    Masters Thesis
    Full Text:
    Rootstocks Abstract approved: ~4K, Barbara M. Reed The availability of new pear rootstocks could be