Alternating temperature (22°C / -1°C) pretreatments were effective for
increasing cold hardiness and recovery of cryopreserved meristems. Combining low
temperature pretreatment with abscisic acid (ABA) or sucrose treatments reduced the
time required for optimal cold hardiness of meristems. Desiccation tolerance was
directly correlated with increased cold hardiness and recovery of...
Oxidative stress that occurs during cryopreservation may be reduced by the addition of antioxidants. Vitamin E (Vit E), Vitamin C (Vit C), glutathione (GSH), lipoic acid (LA), glycine betaine (GB) and polyvinylpyrrolidone (PVP) were applied at four steps of the PVS2 vitrification protocol (pretreatment, loading, rinsing, and regrowth). Shoot tips...
Bacterial contamination is one of the most important problems
both in plant tissue culture research and commercial laboratories. Early
detection of contaminants saves time, effort, and money. Strawberry
runner explants were screened for contaminants during June to August,
1994 and 1995. Most contaminants found were bacterial rather than
fungal. Bacterial...
Roots of greenhouse-grown peppermint (Mentha piperita L.) and spearmint (Mentha spicata L.)
plants and in vitro-grown shoot cultures were inoculated with Verticillium dahliae conidial suspensions to
study symptom development, detection, and elimination of the V. dahliae. There were significant
differences in the symptom expression of control and infected shoot cultures...
Two studies used DNA markers to assess their usefulness in germplasm identification and evaluation of genetic stability in four cryopreserved Rubus accessions that were stored for over 10 years in liquid nitrogen. In the first study, 12 robust Simple Sequence Repeat (SSR) markers were developed from two microsatellite-enriched libraries of...
Genetic stability of micropropagated and cold-stored Fragaria x anannasa cultivar
'Pocahontas' was evaluated by Random Amplified Polymorphic DNA (RAPD) assay.
Plantlets grown for eight months on M & S medium with 5 μM and 15 μM N6-
benzyladenine (BA) were analyzed. No evidence of mutations was seen in over 200...
Improved recovery of cryopreserved meristems and calli from in
vitro currant (Ribes aureum Pursh and R. ciliatum Humb. & Bonpl.) plants
was obtained by two-hour pretreatment with sucrose, proline, abscisic
acid-responsive protein (RABP), and bovine serum albumin (BSA)
solutions. Meristems and calli cold-acclimatized for one week were
immersed in pretreatment...
Red raspberry, Rubus idaeus L., is a valuable crop for the U.S. Pacific Northwest and clonal propagation is required to produce disease-free plants and for germplasm preservation. One challenge of red raspberry micropropagation is the wide variation in growth response among the cultivars. The studies described in this dissertation were...
Corylus avellana L. is a global commodity and a valuable crop for the U.S. Pacific Northwest. A tremendous amount of work has been put into the development of healthy disease free, high yielding and tasty hazelnuts for the in-shell and confectionary markets. Clonal propagation is required to provide nursery trees...
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Barbara M. Reed
Corylus avellana L. is a global commodity and a valuable crop for the U.S
A micropropagation system was developed for hazelnut cultivars and
rootstocks. Culture establishment was affected by explant source, explant collecting
season, and type of explant. The highest frequency of culture initiation and lowest
tissue oxidation and contamination were obtained from internodes with single axillary
buds from grafted greenhouse-grown plants early in...