The extent to which responses to stress are maladaptive or adaptive to the long-term survival of fish remains to be better understood. The aim of this study was to identify differentially expressed genes in the livers of rainbow trout, Oncorhynchus mykiss, responding to an experimental stressor. Gene expression responses were...
This study was undertaken to determine if strontium chloride could be used to create a trans-generational otolith mark in steelhead (Oncorhynchus mykiss). I completed two strontium injection trials and a survey of juvenile steelhead from various steelhead hatcheries. The two trials measured Sr:Ca ratios in otoliths in response to injections...
To best manage Eagle Creek National Fish Hatchery and minimize any negative impacts that the current hatchery program may be having on Endangered Species Act-listed salmonids in the Eagle Creek Basin, I determined if wild fish are being displaced from preferred habitats by hatchery salmonids Oncorhynchus spp. This thesis had...
Depleted species of rockfish (Sebastes spp.) from the Northeast Pacific experience high discard mortality due to "barotrauma," induced from the rapid change in pressure during capture. Research suggests rockfish have the potential to survive barotrauma if immediately recompressed, but the potential for long-term recovery is unknown. In this project, we...
A number of steelhead (Oncorhynchus mykiss) populations are at risk throughout Oregon, including five populations that are federally listed as threatened or endangered. However, little is known about behavior and survival of these fish as they transition from freshwater to seawater. We investigated whether estuarine survival varies on a temporal...
Periodic determinations of plasma hormone levels suggested that changes in interrenal activity are unrelated to changes in thyroidal or gonadal activity during development (smoltification) of juvenile coho salmon (Oncorhynchus kisutch). Moreover, in an experiment where fish were reared under various conditions of crowding and water inflow rate, it appeared that...
Efforts to manage stocks of salmonids in Pacific Northwest stream systems are complicated by the occurrence of several runs of a species thought to represent races rearing sympatrically as juveniles. In order to collect the population statistics needed to properly manage these stocks, managers need a method of identifying juvenile...
The metabolic clearance rate (MCR) of plasma radioactivity after a
single intracardial injection of ³H-cortisol was elevated during the
Spring in yearling coho salmon, Oncorhynchus kisutch. The reasons for
this increase in MCR are not clear, but graphical analysis suggested
that there may be a seasonal correlation between MCR and...
Identifying appropriate units for conservation requires knowledge of evolutionary
patterns and risks of managing at different geographical and genetic scales. I examined
genetic diversity at different geographical scales among 11,400 rainbow trout
(Oncorhynchus mykiss) from 243 locations in 13 major river basins throughout much of
their range and among coho...
Estuaries provide juvenile salmonids with highly productive feeding grounds, refugia from tidal fluctuations and predators, and acclimation areas for smoltification. However, these dynamic, fluctuating salinity environments may also be physiologically stressful to growing juvenile fish. In order to evaluate the costs and benefits of estuarine marshes to juvenile Chinook salmon,...
Detection of L-serine and D,L-alanine by juvenile coho salmon (Oncorhynchus kisutch) was measured in a two-choice Y-trough. Threshold concentration for olfactory
detection of L-serine was 10-8 M for zero-age parr, 1O-7 M for yearling parr, 10-5 M for smolts in late April-early May, and 10-6 M for smolts in June....
Phenotypic similarities of coho salmon (Oncorhynchus kisutch)
stocks from Oregon, Washington, and California were compared using
agglomerative and divisive cluster analyses. The phenotypic characters
evaluated included the following: 1) the isozyme gene frequencies
of transferrin and phosphoglucose isomerase; 2) the life history
characters time of peak spawning and proportion of...
Mean weights, lengths, and condition factors of three isozyme
phenotypes of lactate dehydrogenase (LDH) enzyme differed significantly
for experimentally reared winter steelhead trout (Salmo
gairdneri) fry. Time of emergence from the gravel was unrelated
to LDH phenotype. Relative mortality of the phenotypes between
eyed-egg stage and emergence was unaffected by...
Genetic, ontogenetic, and environmental factors modified characteristic interrenal and glycemic responses to stress in juvenile salmonid fishes. During continuous confinement stress, plasma cortisol rose more quickly in chinook salmon, Oncorhynchus tshawytscha, acclimated to high rather than medium or low temperatures; hyperglycemia following either acute or chronic stress was also highest...
The behavior and stream habitat utilization of juvenile hatchery-reared coho salmon (Ovicozihynchus kisutch) and wild cutthroat trout (Salmo clarki) were examined to assess the nature of interactions between the species potentially resulting from introductions of hatchery coho salmon in streams. Habitat utilization was monitored in Mill Creek, Polk Co., Oregon...
Sablefish, Anoplopoma fimbria, is a valuable North Pacific Ocean species caught in several commercial fisheries and is often discarded due to size or catch limits. Managers must account for the mortality of discarded fish to assess fish populations and harvest impacts, yet discard mortality rates of specific fisheries are generally...
Freshwater (FW) and seawater (SW) acclimated yearling coho salmon, Oncorhynchus kisutch, were subjected to severe confinement stress in FW, SW, or a medium (1/3 SW) that was approximately isosmotic to the fish's blood. Chronic stress caused osmotic imbalances in FW and SW, but not in 1/3 SW. In SW, blood...
The extensive reduction in adult Pacific lamprey (Entosphenus tridentatus) counts at many hydroelectric dams in the northwestern USA signals a substantial decline in lamprey numbers across the entire region in the past 40 to 50 years. Among the many potential causes of this decline, obstruction of migration routes has likely...
Pacific lamprey (Entosphenus tridentatus) are experiencing population declines throughout their range. Xenobiotics could be an important risk factor for lamprey populations. Our goal was to establish if common herbicides, as used in forest management, could affect reproductive fitness. We determined that atrazine was a likely compound of greatest concern to...
The present study investigated the neurocrine and neuroendocrine control of locomotor activity, habitat choice, social behavior, and migratory behavior in juvenile chinook salmon (Oncorhynchus tshawytscha). This was achieved by the manipulation of three neurotransmitter systems; serotonin (5-HT), dopamine (DA), y-amino-n-butyric acid (GABA) and the neuropeptide corticotropin-releasing hormone (CRH). Chemicals were...
Gene expression profiles of tissues and cell-lines can be powerful tools for documenting the genetic response to a particular treatment, such as stressors. However, there is a paucity of information on the genetic stress response in the brain. Therefore, we attempted to profile gene expression in the brain of juvenile...
The present study investigated the effects of stress and exercise on seawater (SW) adaptation and cortisol dynamics in juvenile steelhead (Oncorhynchus mykiss). To examine the effects of stress, fish acclimated to freshwater (FW) were subjected for 3 hours to confinement stress in FW, and subsequently SW (25 ppt) was introduced...
The work presented in this dissertation examines possible
modes of action for growth inhibition by anthropogenic endocrine
disrupting chemicals (EDCs) as well as endogenous hormones
associated with growth in fish. Using the sheepshead minnow (SHM)
(Cyprinodon variegatus) as a model, I developed methods to examine
perturbations in the endocrine axis...
Wild populations of fish are faced with a multitude of stressors, which may include human interaction, toxins, and disease. Benzo(a)pyrene (BaP), a known carcinogen and immunotoxin, has been reported in the stomach contents of juvenile chinook salmon, Oncorhynchus tshawytscha, in urban waterways. We investigated the impact of chronic dietary exposure...
In this thesis, we examined the effects of the exposures to anthropogenic pollutants on the fish, primarily juvenile chinook salmon, immune system using newly and recently developed immune assays. In addition, we developed a new assay for measuring immunocompetence of fish. In the first chapter, the Alamar Blue assay was...
From 1998-2000, laboratory studies were conducted to examine factors that impact saltwater-entry behavior and saltwater preference (SWP) of juvenile chinook salmon, Oncorhynchus tshawytscha. These factors included bacterial kidney disease, stress and the presence of trout, O. mykiss. An additional study investigated the orientation of the startle response of chinook salmon...
Environmental factors such as chemical contamination can have immunomodulatory effects on the immune response of fish and may be contributing to the decline in salmonid populations by augmenting disease susceptibility. Xenobiotics can interfere with the immune system at several levels of complexity, and different immune cells and processes have variable...
Sex steroids, follicle stimulating hormone (FSH),
luteinizing hormone (LH) and gonadotropin releasing hormone
(GnRH) were measured in monosex and mixed sex populations of
rainbow trout during early development. Steroids declined
following fertilization, increased at hatch, then fell and
remained constant thereafter. Trends toward differences in
steroids between males and females...
Sex differentiation in fish is a labile process that allows sex inversion in several species. The inherent capacity of fish germ cells to differentiate into oocytes or spermatocytes constitutes a key factor allowing for functional sex inversion. This thesis set out to determine the mechanism involved in steroid-induced sex differentiation...
Stress, including extreme or rapidly changing temperatures, are known to have deleterious effects on fish health and physiology. This thesis examines the combined effects of elevated acclimation temperature and acute handling stress on the number of antibody producing cells, plasma lysozyme concentrations, and the number of pronephric leukocytes in juvenile...
The development of assays for stress in marine fishes is vital for studying the impacts of bycatch in fisheries and for determining the health of fish being cultured or used in research. This research developed behavioral and physiological assays for stress in juvenile sablefish, Anoplopoma fimbria, a species that comprises...
Electrofishing is a sampling tool commonly used by fisheries researchers. While much is known about the effects of electroshock on fish physiology, consequences to the immune system and disease progression have not received attention. To understand the effects of electroshock on immune function we undertook a comparison of electroshock and...
Juvenile chinook salmon (Oncorhynchus tshawytscha) were experimentally infected with Renibacterium salmoninarum (Rs), the causative agent of bacterial kidney disease (BKD), to investigate the effects of BKD on three aspects of juvenile salmonid performance: (1) predator avoidance ability; (2) smoltification; and (3) physiological responses to stress. For these experiments, fish with...
Three experiments were designed to determine if and how steelhead trout,
Oncorhynchus mykiss, may affect chinook salmon, O. tshawytscha, when they are
confined together as in a raceway or on a barge. We observed groups of chinook and
steelhead together and groups of only chinook in a behavioral experiment to...
The environment under which fish are maintained as broodstock before
reproduction is often stressful; however, the impact of stress on broodstock and gamete
quality is not well known. We investigated the effects of stress over the final stages (i.e.
the 3 months preceding ovulation) of sexual maturation of female rainbow...
Genetic differences between early and late forms of
Alaskan chinook salmon (Oncorhynchus tshawytscha) were
identified using two genetic approaches: mitochondrial
DNA (mtDNA) analysis and protein electrophoresis. The
study populations consisted of early- and late-run chinook
salmon in each of the Kenai and Kasilof rivers in Alaska,
and a single population...
The importance of hormone reservoirs in mature teleost eggs is
unknown. To elucidate the effects of hormones on embryonic development,
steelhead trout eggs, Oncorhynchus mykiss, were immersed in either cortisol,
testosterone, or thyroxine at two different stages of development. Elevated
concentrations of cortisol were detectable in the trout eggs or...