A “smart” tracer is a tracer that provides, directly or through measurement of its concentration or in combination with another compound, at least one “bit” more of information about the environment through which it travels than a conservative tracer. In this study we propose and present the chemical compound resazurin...
Water transient storage zones are hotspots for metabolic activity in streams although the contribution of different types of transient storage zones to the whole-reach metabolic activity is difficult to quantify. In this study we present a method to measure the fraction of the transient storage that is metabolically active (MATS)...
Quantification of the transient storage zone (A[subscript s]) has become critical in stream biogeochemical studies addressed to examine factors controlling nutrient uptake. It is expected that higher A[subscript s] may enhance the interaction between nutrients and biota and thus, increase nutrient uptake. However, results from the literature are controversial. We...
We propose the experimental use of resazurin (Raz) and develop a metabolically active transient storage (MATS) model to include processes that may provide additional information on transient storage from a biogeochemical perspective in stream ecosystems. Raz is a phenoxazine compound that reduces irreversibly to resorufin (Rru) in the presence of...
In a set of streamside mesocosms, stream ecosystem respiration (ER) increased with biofilm biomass and flow heterogeneity (turbulence) generated by impermeable bed forms, even though those bed forms had no hyporheic exchange. Two streamside flumes with gravel beds (single layer of gravel) were operated in parallel. The first flume had...
In this technical note, a steady-state analytical solution of concentrations of a parent solute reacting to a daughter solute, both of which are undergoing transport and multirate mass transfer, is presented. Although the governing equations are complicated, the resulting solution can be expressed in simple terms. A function of the...
We provide an efficient method to estimate processing rates through simple algebraic relationships derived from the transient storage model equations. The method is based on the transport equations, but eliminates the need to calibrate highly uncertain (and intermediate) parameters. We demonstrate that under some common stream transport conditions dispersion does...
Stream functioning includes simultaneous interaction among solute transport, nutrient processing, and metabolism. Metabolism is measured with methods that have limited spatial representativeness and are highly uncertain. These problems restrict development of methods for up-scaling biological processes that mediate nutrient processing. We used the resazurin–resorufin (Raz-Rru) tracer system to estimate metabolism...
The use of smart tracers to study hydrologic systems is becoming more widespread. Smart tracers are compounds that irreversibly react in the presence of a process or condition under investigation. Resazurin (Raz) is a smart tracer that undergoes an irreversible reduction to resorufin (Rru) in the presence of cellular metabolic...
Groundwater–surface-water (GW-SW) interactions in streams are difficult to quantify because of heterogeneity in hydraulic and reactive processes across a range of spatial and temporal scales. The challenge of quantifying these interactions has led to the development of several techniques, from centimeter-scale probes to whole-system tracers, including chemical, thermal, and electrical...
Excess NO₃⁻ in streams is a growing and persistent problem for both inland and coastal ecosystems, and denitrification is the primary removal process for NO₃⁻. Hyporheic zones can have high denitrification potentials, but their role in reach- and network-scale NO₃⁻ removal is unknown because it is difficult to estimate. We...
Surface transient storage (STS) has functional significance in stream ecosystems because it increases solute interaction with sediments. After volume, mean residence time is the most important metric of STS, but it is unclear how this can be measured accurately or related to other timescales and field-measureable parameters. We studied mean...
Headwater streams comprise nearly 90% of the total length of perennial channels in global catchments. They mineralize organic carbon entering from terrestrial systems, evade terrestrial carbon dioxide (CO₂ ), and generate and remove carbon through in-stream primary production and respiration. Despite their importance, headwater streams are often neglected in global...
Full Text:
-2427.2003.01062.x.
Battin, T. J., L. A. Kaplan, S. Findlay, C. S. Hopkinson, E. Martí, A. I. Packman, J. D
In field studies of solute transport, transient storage within lateral cavities and other stream features generates
breakthrough curves (BTCs) with pronounced and persistent skewness. Current solute transport
theory requires that the coefficient of skewness (CSK) decrease over time because the system eventually
reaches Fickian conditions. However, published data show that...