Index Catalog // ScholarsArchive@OSU

Method development for extraction and purification of dermal RNA from FVB/N mice treated with environmental PAH mixtures

Creator:: Larkin, Andrew, Siddens, Lisbeth K., Krueger, Sharon K., Williams, David E., and Baird, William M.
Abstract:: Epidermal RNA samples from FVB/N mice treated with PAH standards and environmental PAH mixtures were harvested and purified using Trizol extraction and RNeasy mini prep kit purification. Epidermal RNA integrity was low (RIN mean 3.5), indicating significant RNA degradation and the need to modify handling, collection, and processing of skin...
Resource Type:: Research Paper
Full Text:: . Williamsb, William M. Bairdb,* a Department of Bioresource Research, Oregon State University, Corvallis

Application of a fuzzy neural network model in predicting polycyclic aromatic hydrocarbon-mediated perturbations of the Cyp1b1 transcriptional regulatory network in mouse skin

Creator:: Larkin, Andrew, Siddens, Lisbeth K., Krueger, Sharon K., Tilton, Susan C., Waters, Katrina M., Williams, David E., and Baird, William M.
Abstract:: Polycyclic aromatic hydrocarbons (PAHs) are present in the environment as complex mixtures with components that have diverse carcinogenic potencies and mostly unknown interactive effects. Non-additive PAH interactions have been observed in regulation of cytochrome P450 (CYP) gene expression in the CYP1 family. To better understand and predict biological effects of...
Resource Type:: Article
Full Text:: . Waters c,e, David E. Williams a,c,d,f,⁎, William M. Baird a,c,f a Department of Environmental and

Mechanism-based classification of PAH mixtures to predict carcinogenic potential

Creator:: Tilton, Susan C., Siddens, Lisbeth K., Krueger, Sharon K., Larkin, Andrew J., Löhr, Christiane V., Williams, David E., Baird, William M., and Waters, Katrina M.
Abstract:: We have previously shown that relative potency factors and DNA adduct measurements are inadequate for predicting carcinogenicity of certain polycyclic aromatic hydrocarbons (PAHs) and PAH mixtures, particularly those that function through alternate pathways or exhibit greater promotional activity compared to benzo[a]pyrene (BaP). Therefore, we developed a pathway based approach for...
Resource Type:: Article
Full Text:: Christiane V. Löhr § , David E. Williams* ,†,‡ , William M. Baird* ,†, , Katrina M. Waters* ,¶ 4 5

Cytochrome P450 1b1 in polycyclic aromatic hydrocarbon (PAH)-induced skin carcinogenesis: Tumorigenicity of individual PAHs and coal-tar extract, DNA adduction and expression of select genes in the Cyp1b1 knockout mouse

Creator:: Siddens, Lisbeth K., Bunde, Kristi L., Harper, Tod A., McQuistan, Tammie J., Löhr, Christiane V., Bramer, Lisa M., Waters, Katrina M., Tilton, Susan C., Krueger, Sharon K., Williams, David E., and Baird, William M.
Abstract:: FVB/N mice wild-type, heterozygous or null for Cyp 1b1 were used in a two-stage skin tumor study comparing PAH, benzo[a]pyrene (BaP), dibenzo[def,p]chrysene (DBC), and coal tar extract (CTE, SRM 1597a). Following 20 weeks of promotion with TPA the Cyp 1b1 null mice, initiated with DBC, exhibited reductions in incidence, multiplicity,...
Resource Type:: Article
Full Text:: , David E. Williams a,b,c,d,⁎, William M. Baird a,b,d a Department of Environmental and Molecular

Polycyclic aromatic hydrocarbons as skin carcinogens: Comparison of benzo[a]pyrene, dibenzo[def,p]chrysene and three environmental mixtures in the FVB/N mouse

Creator:: Siddens, Lisbeth K., Larkin, Andrew, Krueger, Sharon K., Bradfield, Christopher A., Waters, Katrina M., Tilton, Susan C., Pereira, Cliff B., Lohr, Christiane V., Arlt, Volker M., Phillips, David H., Williams, David E., and Baird, William M.
Abstract:: The polycyclic aromatic hydrocarbon (PAH), benzo[a]pyrene (BaP), was compared to dibenzo[def,p]chrysene (DBC) and combinations of three environmental PAH mixtures (coal tar, diesel particulate and cigarette smoke condensate) using a two stage, FVB/N mouse skin tumor model. DBC (4 nmol) was most potent, reaching 100% tumor incidence with a shorter latency...
Resource Type:: Article
Full Text:: i, David E. Williams a,b,c,g,⁎, William M. Baird a,b,g a Department of Environmental and Molecular

Transplacental Carcinogenesis with Dibenzo[def,p]chrysene (DBC): Timing of Maternal Exposures Determines Target Tissue Response in Offspring

Creator:: Shorey, Lyndsey, Castro, David J., Baird, William, Siddens, Beth, Lohr, Christiane, Matzke, Melissa, Waters, Katrina M., Corley, Richard A., and Williams, David E.
Abstract:: Dibenzo[def,p]chrysene (DBC) is a transplacental carcinogen in mice (15 mg/kg; gestation day (GD) 17). To mimic residual exposure throughout pregnancy, dams received 4 smaller doses of DBC (3.75 mg/kg) on GD 5, 9, 13 and 17. This regimen alleviated the previously established carcinogenic responses in the thymus, lung, and liver....
Resource Type:: Article
Full Text:: . Castro1,3, William Baird1,4,5, Beth Siddens1,4,5, Christiane Löhr4,5,6, Melissa Matzke7, Katrina M

3,3'-Diindolylmethane Induces G₁ Arrest and Apoptosis in Human Acute T-Cell Lymphoblastic Leukemia Cells

Creator:: Shorey, Lyndsey E., Hagman, Amanda M., Williams, David E., Ho, Emily, Dashwood, Roderick H., and Benninghoff, Abby D.
Abstract:: Certain bioactive food components, including indole-3-carbinol (I3C) and 3,3'-diindolylmethane (DIM) from cruciferous vegetables, have been shown to target cellular pathways regulating carcinogenesis. Previously, our laboratory showed that dietary I3C is an effective transplacental chemopreventive agent in a dibenzo[def,p]chrysene (DBC)-dependent model of murine T-cell lymphoblastic lymphoma. The primary objective of the...
Resource Type:: Article
Full Text:: Apoptosis in Human Acute T-Cell Lymphoblastic Leukemia Cells Lyndsey E. Shorey1,2, Amanda M. Hagman3, David

WongCarmenPublicHealthHumanSciencesEffectsSulforaphane.pdf

Creator:: Wong, Carmen P., Hsu, Anna, Buchanan, Alex, Palomera-Sanchez, Zoraya, Beaver, Laura M., Houseman, E. Andres, Williams, David E., Dashwood, Roderick H., and Ho, Emily
Abstract:: Epigenetic changes, including aberrant DNA methylation, result in altered gene expression and play an important role in carcinogenesis. Phytochemicals such as sulforaphane (SFN) and 3,3'-diindolylmethane (DIM) are promising chemopreventive agents for the treatment of prostate cancer. Both have been shown to induce re-expression of genes, including tumor suppressor genes silenced...
Full Text:: -Sanchez1,2, Laura M. Beaver1,2, E. Andres Houseman1, David E. Williams2,3, Roderick H. Dashwood2,3, Emily

WongCarmenPublicHealthHumanSciencesEffectsSulforaphane_SupportingInformation.zip

Creator:: Wong, Carmen P., Hsu, Anna, Buchanan, Alex, Palomera-Sanchez, Zoraya, Beaver, Laura M., Houseman, E. Andres, Williams, David E., Dashwood, Roderick H., and Ho, Emily
Abstract:: Epigenetic changes, including aberrant DNA methylation, result in altered gene expression and play an important role in carcinogenesis. Phytochemicals such as sulforaphane (SFN) and 3,3'-diindolylmethane (DIM) are promising chemopreventive agents for the treatment of prostate cancer. Both have been shown to induce re-expression of genes, including tumor suppressor genes silenced...

Effects of Sulforaphane and 3,3'-Diindolylmethane on Genome-Wide Promoter Methylation in Normal Prostate Epithelial Cells and Prostate Cancer Cells

Creator:: Wong, Carmen P., Hsu, Anna, Buchanan, Alex, Palomera-Sanchez, Zoraya, Beaver, Laura M., Houseman, E. Andres, Williams, David E., Dashwood, Roderick H., and Ho, Emily
Abstract:: Epigenetic changes, including aberrant DNA methylation, result in altered gene expression and play an important role in carcinogenesis. Phytochemicals such as sulforaphane (SFN) and 3,3′-diindolylmethane (DIM) are promising chemopreventive agents for the treatment of prostate cancer. Both have been shown to induce re-expression of genes, including tumor suppressor genes silenced...
Resource Type:: Article

ScholarsArchive@OSU

Method development for extraction and purification of dermal RNA from FVB/N mice treated with environmental PAH mixtures

Application of a fuzzy neural network model in predicting polycyclic aromatic hydrocarbon-mediated perturbations of the Cyp1b1 transcriptional regulatory network in mouse skin

Mechanism-based classification of PAH mixtures to predict carcinogenic potential

Cytochrome P450 1b1 in polycyclic aromatic hydrocarbon (PAH)-induced skin carcinogenesis: Tumorigenicity of individual PAHs and coal-tar extract, DNA adduction and expression of select genes in the Cyp1b1 knockout mouse

Polycyclic aromatic hydrocarbons as skin carcinogens: Comparison of benzo[a]pyrene, dibenzo[def,p]chrysene and three environmental mixtures in the FVB/N mouse

Transplacental Carcinogenesis with Dibenzo[def,p]chrysene (DBC): Timing of Maternal Exposures Determines Target Tissue Response in Offspring

3,3'-Diindolylmethane Induces G₁ Arrest and Apoptosis in Human Acute T-Cell Lymphoblastic Leukemia Cells

WongCarmenPublicHealthHumanSciencesEffectsSulforaphane.pdf

WongCarmenPublicHealthHumanSciencesEffectsSulforaphane_SupportingInformation.zip

Effects of Sulforaphane and 3,3'-Diindolylmethane on Genome-Wide Promoter Methylation in Normal Prostate Epithelial Cells and Prostate Cancer Cells

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