Desferrioxamine B (DFOB) was reversibly and covalently conjugated to polystyrene microspheres via N-succinimidyl 3-(2-pyridyldithio)propionate (SPDP) crosslinker. DFOB retained post-immobilization activity with at least 89% able to bind Fe(III)-citrate under pH 7.4 and a citrate:iron molar ratio = 100:1. Comparing iron capture by DFOB-activated beads and sulfhydryl (−SH) control beads demonstrated...
Many industrially important reactions use immobilized enzymes in non-aqueous, organic
systems, particularly for the production of chiral compounds such as pharmaceutical precursors.
The addition of a spacer molecule (“tether”) between a supporting surface and enzyme often
substantially improves the activity and stability of enzymes in aqueous solution. Most “long”
linkers...