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Reversible Covalent Binding of Desferrioxamine B (DFOB) to 2 Polystyrene Microspheres for the Chelation of Aqueous Iron Citrate

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https://ir.library.oregonstate.edu/concern/articles/z316q939s

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  • Desferrioxamine B (DFOB) was reversibly and covalently conjugated to polystyrene microspheres via N-succinimidyl 3-(2-pyridyldithio)propionate (SPDP) crosslinker. DFOB retained post-immobilization activity with at least 89% able to bind Fe(III)-citrate under pH 7.4 and a citrate:iron molar ratio = 100:1. Comparing iron capture by DFOB-activated beads and sulfhydryl (−SH) control beads demonstrated exclusive iron chelation by surface DFOB molecules. Support regeneration was achieved by cleaving the disulfide bonds linking the spent chelators to the surface with dithiothreitol (DTT) and then recoupling to fresh DFOB. This process did not significantly change iron uptake after two uses. The Langmuir isotherm described the adsorption data with maximum iron coverage of 3.7 × 10⁻³ mmolFe(III)/gadsorbent. DFOB immobilization significantly reduced the chelation rate; 30 and 180 min were required to reach equilibrium chelation levels with free and immobilized DFOB, respectively. The chelation rate decrease was attributed to a surface hindrance of DFOB’s iron complexation mechanism and the reduced access to the supernatant bulk. It is anticipated that future systems tailored for specific DFOB applications can utilize the developed reversible attachment for flexible chelator regeneration, in addition to support reusability and preservation.
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  • 62
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  • 37
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  • 1520-5045

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