Desferrioxamine B (DFOB) was reversibly and covalently conjugated to polystyrene microspheres via N-succinimidyl 3-(2-pyridyldithio)propionate (SPDP) crosslinker. DFOB retained post-immobilization activity with at least 89% able to bind Fe(III)-citrate under pH 7.4 and a citrate:iron molar ratio = 100:1. Comparing iron capture by DFOB-activated beads and sulfhydryl (−SH) control beads demonstrated...