Index Catalog // ScholarsArchive@OSU

Biosynthesis of aminodicarboxylic acids in Acetobacter suboxydans

Creator:: Maragoudakis, Michael Emmanuel
Resource Type:: Dissertation

Synthesis of Glutamic-γ-semialdehyde in Escherichia coli : isolation of a low molecular weight compound required for in vitro synthesis

Creator:: Hartz, Judith Ann
Abstract:: Preliminary work on a crude in vitro system for glutamic-γ-semialdehyde synthesis in Escherichia coli has been described. The cell extract synthesis of GSA is inhibited by proline; other allosteric characteristics observed include sigmoidal substrate variation and inhibition plots of activity. The membrane fraction and a low molecular weight compound in...
Resource Type:: Masters Thesis

Terminal reactions in tryptophan biosynthesis in yeast

Creator:: Saunders, Priscilla Prince
Resource Type:: Masters Thesis

Biosynthesis of yeast sterols

Creator:: Turner, Jan Ross
Abstract:: An enzyme system from cell-free yeast homogenates mediating the transfer of the methyl group from S-adenosylmethionine-methyl-C¹⁴ or methionine-methyl-C¹⁴ to C-28 of ergosterol has been studied. The effects of divalent cations, suithydryl inhibitors and reducing substances on the activity of the enzyme system have been investigated. It was established that for...
Resource Type:: Masters Thesis

The terminal reactions in the biosynthesis of methionine in Saccharomyces cerevisiae

Creator:: Pigg, C. JoAnne
Abstract:: The terminal reactions in the biosynthetic pathway of methionine in Saccharomyces cerevisiae were investigated in this study. Analyses of a number of methionine auxotrophs for biochemical deficiences established four mutant groups. It was observed from qualitative feeding experiments that some auxotrophs were able to utilize S-adenosylmethionine (AM) and S-adenosyl-homocysteine (AH)...
Resource Type:: Dissertation

The cysteine to homocysteine conversion in Saccharomyces cerevisiae

Creator:: Sorsoli, Wayne Aldo
Abstract:: The mechanism of transsulfuration and the role of cystathionine in the biosynthesis of methionine in yeast were investigated. Saccharomyces were shown to accumulate cystathionine by use of the ³⁵S labeled compound. Cell-free extracts of the wildtype clone as well as methionine auxotrophs were shown to cleave cystathionine. The enzymatically produced...
Resource Type:: Dissertation

Regulation of the terminal reactions for methionine biosynthesis in yeast

Creator:: Spence, Kemet Dean
Abstract:: Studies were carried out to elucidate the mechanism of enzymatic control of methionine biosynthesis in Saccharomyces cerevisiae. Enzymatic studies demonstrated that, in addition to the methionine activating enzyme, the S-adenosylmethionine:homocysteine transmethylase enzyme was induced during cultivation in the presence of excess methionine. A similar, though reduced effect was observed when...
Resource Type:: Dissertation

Enzymatic studies on some methionine auxotrophs of yeast

Creator:: Pigg, C. JoAnne
Resource Type:: Masters Thesis

The biosynthetic precursor of ethylene in Penicillium digitatum Saccardo

Creator:: Jacobsen, Donald Weldon
Abstract:: An attempt has been made to elucidate the biosynthetic precursor of ethylene in the mold P. digitatum. By application of basic radiotracer methodology and the utilization of several specifically labeled radiochemical substrates, it has been possible to demonstrate that the internal carbon atoms of C-4 dicarboxylic acids such as succinate,...
Resource Type:: Masters Thesis

The role of some of the Krebs Cycle reactions in the biosynthetic functions of Thiobacillus thioparus

Creator:: Still, Gerald G., 1933-
Abstract:: Aseptic radiorespirometry has been used to examine the utilization of external carbon sources by proliferating Thiobacillus thioparus cells. These studies reveal that glucose, galactose, mannose, fructose, ribose, DL-glutamate, and L-aspartate were not utilized by this chemoautotrophic organism. However, it has been shown that trace amounts of acetate, glycine, DL-serine, DL-alanine,...
Resource Type:: Dissertation

ScholarsArchive@OSU

Biosynthesis of aminodicarboxylic acids in Acetobacter suboxydans

Synthesis of Glutamic-γ-semialdehyde in Escherichia coli : isolation of a low molecular weight compound required for in vitro synthesis

Terminal reactions in tryptophan biosynthesis in yeast

Biosynthesis of yeast sterols

The terminal reactions in the biosynthesis of methionine in Saccharomyces cerevisiae

The cysteine to homocysteine conversion in Saccharomyces cerevisiae

Regulation of the terminal reactions for methionine biosynthesis in yeast

Enzymatic studies on some methionine auxotrophs of yeast

The biosynthetic precursor of ethylene in Penicillium digitatum Saccardo

The role of some of the Krebs Cycle reactions in the biosynthetic functions of Thiobacillus thioparus

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