Alternating temperature (22°C / -1°C) pretreatments were effective for
increasing cold hardiness and recovery of cryopreserved meristems. Combining low
temperature pretreatment with abscisic acid (ABA) or sucrose treatments reduced the
time required for optimal cold hardiness of meristems. Desiccation tolerance was
directly correlated with increased cold hardiness and recovery of...
Improved recovery of cryopreserved meristems and calli from in
vitro currant (Ribes aureum Pursh and R. ciliatum Humb. & Bonpl.) plants
was obtained by two-hour pretreatment with sucrose, proline, abscisic
acid-responsive protein (RABP), and bovine serum albumin (BSA)
solutions. Meristems and calli cold-acclimatized for one week were
immersed in pretreatment...
Bacterial contamination is one of the most important problems
both in plant tissue culture research and commercial laboratories. Early
detection of contaminants saves time, effort, and money. Strawberry
runner explants were screened for contaminants during June to August,
1994 and 1995. Most contaminants found were bacterial rather than
fungal. Bacterial...