RNA silencing is an evolutionarily conserved system that
functions as an antiviral mechanism in higher plants and
insects. To counteract RNA silencing, viruses express
silencing suppressors that interfere with both siRNA- and
microRNA-guided silencing pathways. We used comparative
in vitro and in vivo approaches to analyse the molecular
mechanism of...
This study described how and why a Korean elementary teacher taught mathematics as he did. Specifically, the study sought to describe his beliefs about the teaching and learning of mathematics and relate them to patterns of classroom interaction and norms.
An ethnographic inquiry guided the study of one third grade...
The purpose of this investigation was to describe tenth grade students' views
of the purposes of geometric proof within the context of their learning. Classroom
observations, the curriculum, assessment tools, journal questions, and a
preconceptions questionnaire were used to provide context for the views expressed by
students from a single...
It has been held that heuristic training alone is not enough for developing one's mathematical thinking. One missing component is a mathematical point of view. Many educational researchers have proposed problem-based curricula to improve students' views of mathematical thinking. The present study reports findings regarding effects of a problem-based calculus...
Papain-like leader proteases are found in diverse families of human, animal, plant, and fungal positive-strand RNA viruses. In addition to autocatalytic processing, these proteases play a variety of roles in the virus life cycle. In particular, the leader protease (L-Pro) of a prototype member of the Closterovirus genus, Beet yellows...
We constructed an experimental apparatus that used lasers to provide the probe beams for measuring the transient absorption kinetics of bacterioplankton that contain proteorhodopsin, a microbial protein that binds retinal and is analogous to animal rhodopsin. With this approach we were able to observe photocycles characteristic of functioning retinylidene ion...
RNA silencing pathways are required for a wide variety of processes in most
eucaryotes. In plants, small-interfering RNA (siRNA) arising from transposons and other
repetitive sequences is associated with heterochromatin formation and maintenance.
MicroRNAs and trans-acting siRNAs encoded at discrete loci function as negative regulators of
gene expression by triggering...
Previous research has shown a gap between chemistry students' conceptual
and computational understandings of chemistry topics such as gas laws,
equilibrium, and reactions. This qualitative study examined the conceptual and
computational understandings of stoichiometry of college students enrolled in a
large lecture Introductory Chemistry course. Factors that might influence students'...
Stable expression of transgenes is required for commercial uses of genetically engineered trees. To better understand the stability of transgene expression under field conditions, we studied transgene expression and RNA interference (RNAi)-induced transgene suppression in 2,480 transgenic poplars (460 transgene insertion events) over three years. Stability of expression was assessed...