Escherichia coli double-strand uracil-DNA glycosylase (Dug) was purified to apparent homogeneity from bacteria that were defective in uracil-DNA glycosylase (Ung). After cloning the dug gene, recombinant Dug was overexpressed, purified, and characterized with respect to activity, substrate specificity, product DNA binding, and mechanism of action. Purified Dug excised both uracil...
The focus of this study is to develop a general and low-cost solution-based
process to fabricate micro- and nano-structured semiconductors that are suitable
for electronics. This process uses simple metal halide precursors dissolved in a
solvent (organic or aqueous) and is capable of forming uniform and continuous
thin films via...
The effect of cosputtered catalyst on growth and alignment of carbon nanotubes (CNTs) grown by plasma enhanced chemical vapor deposition (PECVD) was investigated. Aligned CNTs were observed using a cosputtered catalytic metal deposited directly on boro-aluminosilicate glass. Catalytic metal alloys were sputtered directly onto the substrate using magnetron sputtering. Deposition...
Ferrites have been used for various high frequency applications as bulk
materials. These applications, however, are limited to large dimension devices. In
this thesis, thin film ferrites were deposited from a low temperature solution-based
deposition process that is suitable for micro-scale high frequency applications. The
low temperature nature of this...
The concept of reducing laboratory operations in scale such that they fit on a microfluidic chip has been met with great enthusiasm. Lab-on-a-chip devices promise to be cost effective to operate due to reduced reagent consumption, have the potential to offer shorter analysis times due to their short path lengths,...
Torrubiella is a genus of entomopathogenic fungi in the Clavicipitaceae
(Ascomycota). Species of the genus produce superficial perithecia on a loose mat of
hyphae that forms directly on the host. The genus is estimated to contain about 70
species. Torrubiella is hypothesized to be a close relative of Cordyceps because...
The dependence of the substrate resistance, R[subscript sub], for MOS transistor RF modeling on transistor biasing and layout is studied from device simulations and measurements. Though R[subscript sub] is found to be bias dependent, the error incurred by assuming a constant value equal to the DC resistance is not significant....
DNA damage, if not repaired, can become a mutation. Mutation
accumulation is associated with initiation and progression of tumorigenesis. DNA
mismatch repair (MMR) is required for maintaining genetic stability by repairing
replication errors. Biochemical studies have shown that MMR also recognizes
mismatch-causing DNA lesions, suggesting the role of MMR in...
PCR-based codon-specific random mutagenesis and site-specific mutagenesis were performed to construct a library of 18 amino acid changes at Arg276 in the conserved leucine-loop of the core catalytic domain of human uracil-DNA glycosylase (UNG). Each Arg276 mutant was then overproduced in E. coli cells and purified to apparent homogeneity by...