Fluorescent proteins have emerged as an essential toolset for bioimaging, creating a demand for engineering proteins with new and improved fluorescent properties. In this thesis, I explore the atomistic structure of REX-GECO1, a newly engineered protein biosensor that has unique optical properties. Since this protein has no available crystal structure,...
Submarine glacial landforms in fjords are imprints of the dynamic behaviour of marine-terminating glaciers and are informative about their most recent retreat phase. Here we use detailed multibeam bathymetry to map glacial landforms in Petermann Fjord and Nares Strait, northwestern Greenland. A large grounding-zone wedge (GZW) demonstrates that Petermann Glacier...
Vortex shedding is a phenomenon relevant to any industry dealing with fluid flow. The shed vortices often produce oscillatory forces, which have been suspect in the catastrophic failure of airplanes and bridges alike. To prevent further engineering failures a better understanding of the underlying physics is needed. It has been...
As a key historic component of prairies in the Pacific Northwest, Camassia spp. should play a role in the restoration of these ecosystems. To do so effectively, further study of Camassia spp. propagation is warranted. Thus, the growth, N uptake and allocation, and seasonal thermoperiodicity of Camassia spp. was examined....
Tourism is one of the largest industries in the world, and causes many cultural, environmental, and economic problems. In this thesis, I explore ethics in the field of tourism studies, and identify three ethical models used to discuss ethics in tourism, which are: business ethics, alternative tourism, and philosophical ethics....
On January 21, 2017, the United States bore witness to the largest single-day demonstration in history—the Women’s March. This mixed methods study uses a combination of quantitative and qualitative data in an attempt to predict participation in this infamous protest. I divulge the ways in which the 2017 Women’s March...
Mycobacterium avium subsp. hominissuis (MAH) belongs to the most-clinically significant non-tuberculous mycobacterial (NTM) pathogens with constant increase in disease prevalence, mainly in several industrialized western countries where tuberculosis is less prevalent. Upon entry into the alveolar space, MAH is engulfed by resident-macrophages, where the pathogen adapts to the hostile phagosomal...
Preschool outdoor play environments (OPEs) are unique spaces that, when carefully designed, can encourage child-initiated play resulting in jumping, chasing, and exploration of materials and natural spaces (Moore, 1996). Specifically, elements of the OPE can facilitate movement and increase physical activity (Baek et al., 2015) through engaged, child-initiated play. Children...
Few navigation-assistance technologies for vision impaired individuals are practical due to inhibiting factors such as cost, comfort, convenience, and overall usefulness of the assistance. This thesis will present the design and implementation of a navigation assistance system intended to address these concerns. The presented system improves on existing technologies by...
Soft matter encapsulates a large array of materials ranging from electronics, plastics, and cosmetics to the biofluids making up all organisms. One of the most important properties of these materials that dictate their functionality is their rheology, which is the flow and deformation behavior in response to stress. The focus...