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  • Nongenomic Actions of Steroid Hormones in the Ovine Endometrium

    Creator:
    Ireton, Kyle E.
    Abstract:
    The steroid hormone estrogen plays a crucial role in regulating certain mammalian tissues, especially female reproductive systems. Estrogen effects physiological changes in the cell by altering transcription activity for certain target genes, as a consequence of interactions between estradiol-17β (E2) and its nuclear receptor (nER). This mechanism of cellular control...
    Resource Type:
    Honors College Thesis
    Full Text:
    uterine horns. Recovered tissues were placed immediately in an ice cold homogenization buffer of 25

  • Cyclic nucleotides, cyclic nucleotide phosphodiesterase, protein kinase, and phosphoprotein phosphatase in the central nervous system of Manduca sexta

    Creator:
    Albin, Edward Elmo
    Abstract:
    Adenosine 3', 5' -cyclic monophosphate (cyclic AMP, cAMP) and guanosine 3', 5' -cyclic monophosphate (cyclic GMP, cGMP) were quantitated in the central nervous system (CNS) of the insect Manduca sexta by competitive protein binding techniques. The M. sexta CNS was found to contain a strikingly high level of cGMP, about...
    Resource Type:
    Dissertation

  • Investigation of the roles of viral proteinases in the vaccinia virus life cycle

    Creator:
    Page, Jessica M.
    Abstract:
    An inducible, mutant virus, designated vvtetO:I7L/G1L, was used to study the morphogenic proteolysis step of the vaccinia virus life cycle. The vvtetO:I7L/G1L controlled the expression of two genes, I7L, a cysteine proteinase, and G1L, a putative metalloproteinase. These proteins are involved in the maturation of viral core proteins, p4a, p4b,...
    Resource Type:
    Honors College Thesis
    Full Text:
    homogenization allows cell lysis and release of the virus. The supernatant was layered on a 36% sucrose cushion

  • Interaction of the human Voltage-Dependent Anion Channel 1 protein (VDAC-1) with Mycobacterium avium and its role in bacterial survival within phagocytic cells

    Creator:
    Pham, Tuan
    Abstract:
    M. avium is an opportunistic pathogen that primarily infects macrophages. In order to survive within the macrophage, M. avium secretes proteins into the host cell cytoplasm to inhibit specific functions such as phagosome acidification, altering pathways as well as initiating apoptosis. However, little is known about how those secreted proteins...
    Resource Type:
    Honors College Thesis
    Full Text:
    37oC, 5% CO2, macrophages were removed by scrapping and resuspended in homogenization buffer (1M

  • Investigating the age-related loss of endoplasmic reticulum stress response

    Creator:
    Fallahi, Anahita
    Abstract:
    The endoplasmic reticulum (ER) is a crucial organelle in the cell where protein folding and processing occurs, and conditions that negatively affect ER functions are highly detrimental to the cell. The accumulation of unfolded/misfolded proteins in the ER leads to a condition known as ER Stress. The ER has developed...
    Resource Type:
    Honors College Thesis
    Full Text:
    . Following liver homogenization, cell viability was measured using trypan blue dye exclusion and

  • Nucleic acid polymerases associated with neoplasms

    Creator:
    Kiessling, Ann Drue
    Abstract:
    RNA tumor viruses, such as avian myeloblastosis virus (AMV) and MC29 virus, cause malignant transformation of the cells they infect. Transformed cells are able to continually produce progeny virus particles. Evidence is given that RNA-dependent RNA polymerase is present in virus infected cells. RNA-dependent RNA polymerase isolated from AMV-infected myeloblasts...
    Resource Type:
    Dissertation
    Full Text:
    of the initial homogenization. Organ tissue was placed in liquid nitro- gen in a mortar and ground

  • Cytokinin biosynthesis in crown gall tumors

    Creator:
    Akiyoshi, Donna E.
    Abstract:
    Using combined HPLC-RIA, cytokinin levels in a series of tobacco crown gall tumors, having altered morphologies as a result of transposon mutagenesis were examined. Average trans-ribosylzeatin levels in the shoot-producing tumors (tms mutants) and root-producing tumors (tmr mutants) were 1400 pmol/gm and 0.97 pmol/gm, respectively. Cytokinin/auxin ratios were highest in...
    Resource Type:
    Dissertation
    Full Text:
    ]Kinetin riboside was added as an internal standard prior to homogenization and was measured after HPLC

  • Carnitine and derivatives in embryonic chick tissues

    Creator:
    Casillas, Edmund Rene
    Abstract:
    Carnitine, acetylcarnitine, and long-chain acylcarnitine concentrations were determined for heart, brain, and liver of chick embryos during various stages of development. The total carnitine concentration was approximately the same in all three organs and showed only small variations during development. Acetylcarnitine was not detected in any organ until the seventeenth...
    Resource Type:
    Dissertation
    Full Text:
    by centrifugation, was resuspended in 10-12 ml distilled water by homogenization. An aliquot of

  • Reactions of horseradish peroxidase with the isothiocyanates of horseradish oil

    Creator:
    Darussamin, Asril
    Abstract:
    Horseradish peroxidase (HRP) has been investigated extensively and for many years. In spite of this it still exhibits unexplained anomalies. Among these is variation in the reported number of isozymes (from 7 to 42), and reports of instability of the isozyme patterns. Since horseradish is a rich source of organic...
    Resource Type:
    Dissertation
    Full Text:
    . Maehly (1955) in describing the isolation of crystalline HRP warned that homogenization must be done

  • Chromatin structure : possible regulatory role in gene expression

    Creator:
    Herman, Timothy Merle
    Abstract:
    Chromatin DNA sequences intimately associated with the "core" of the basic, repeating chromatin subunit are shown to be relatively resistant to digestion by micrococcal nuclease. Chromatin DNA sequences lying between adjacent chromatin subunits, as well as DNA sequences in regions of chromatin which are free of chromatin subunits, are subject...
    Resource Type:
    Dissertation
    Full Text:
    resuspended in 10 ml of 10 mM Tris HCI, pH 7.5, 0.7 mM EDTA and disrupted by homogenization for 1 min at a