Avian myeloblastosis virus RNA was fractionated into a high
molecular weight RNA and a low molecular weight RNA fraction by
sucrose density centrifugation. The larger RNA component had a
sedimentation constant of 65S and the low molecular weight RNA
about 4S. The low molecular RNA has two biological properties of...
Preliminary work on a crude in vitro system for glutamic-γ-semialdehyde
synthesis in Escherichia coli has been described. The
cell extract synthesis of GSA is inhibited by proline; other allosteric
characteristics observed include sigmoidal substrate variation and
inhibition plots of activity. The membrane fraction and a low molecular
weight compound in...
The effect of streptomycin on an early reaction of proline
synthesis, the production of glutamic-γ-semialdehyde, in resting
cell suspensions of antibiotic-sensitive, resistant, and dependent
strains of Escherichia coli was examined, and the effect on glutamic-γ-semialdehyde production was found to correspond to the growth
response of the organisms to streptomycin. Inhibition...
In vitro studies of Mobam (4-benzo(b)thienyl N-methylcarbamate) metabolism have demonstrated that at least three ether soluble metabolites are formed in a microsomal system fortified with reduced triphosphopyridine nucleotide (NADPH). One of the metabolites was identical to 4HBT (4-hydroxy benzothiophene), the phenol of Mobam. The other two metabolites did not possess...
In vitro studies of Mobam (4-benzo(b)thienyl N-methylcarbamate)
metabolism have demonstrated that at least three ether soluble
metabolites are formed in a microsomal system fortified with reduced
triphosphopyridine nucleotide (NADPH). One of the metabolites was
identical to 4HBT (4-hydroxy benzothiophene), the phenol of Mobam.
The other two metabolites did not possess...
Reports of investigations on nucleases in arterial tissue are
few in number. The present study is concerned with the purification
and characterization of an aortic ribonuclease.
An endonuclease (ARNase I) isolated from the bovine aorta has
been purified 4611 fold by means of fractionation with ethanol, acid
extraction, isoionic precipitation,...
The binding of fifteen hydrocarbons to native DNA
has been investigated. The hydrocarbons were chosen to
test a size criterion for hydrocarbon - DNA complexes. It
has been found that hydrocarbons, which are small enough
to intercalate between the base pairs of a distorted Watson -
Crick model of DNA,...
A single oral dose of DDT, ranging from 40 to 200 mg/kg, had
a drastic effect on the in vivo acetate metabolism in rats. The normal
elimination pattern of the expired ¹⁴CO₂ from ¹⁴C-labelled
acetate was distorted, and the rate of output of the ¹⁴CO₂ was decreased.
The ratio of...
A fluorescent dye, 1-dimethylaminonaphthalene-5-sulfonyl
chloride (dansyl chloride), was covalently bonded to polylysine. The
dansyl tagged polylysine was complexed to DNA by gradient dialysis.
The polarization and intensity of fluorescence of the dansyl and the
turbidity of the complex were measured as a function of salt concentration
for a variety of...
The thermophilic aldolase from Bacillus stearothermophilus
(NCA 2184) has been purified to a specific activity of 45.5 μ-moles
of fructose-1, 6-diphosphate cleaved per minute per mg of enzyme,
representing a 624-fold increase over the crude extract. The preparation appeared to be homogenous by sedimentation velocity ultra-centrifugation
and by a specific...