Bumble bees (Hymenoptera: Apidae) are important native pollinators in wild and agricultural systems, and are one of the few groups of native bees commercially bred for use in the pollination of a range of crops. In recent years, declines in bumble bees have been reported globally. One factor implicated in...
In recent years, red raspberry production in Washington and British Columbia has been severely limited by a newly emerged virus complex that causes symptoms of crumbly fruit. The complex is comprised of three viruses: Raspberry bushy dwarf virus (RBDV), Raspberry leaf mottle virus (RLMV) and Raspberry latent virus (RpLV). Both...
Landscape heterogeneity is thought to differ among farm management types (i.e. organic and conventional), and this difference is hypothesized to result in variations in pest control by natural enemies. However, it is unclear if these variations in pest control are driven by landscape structure or by farm management practices themselves....
Bees provide vital ecosystem services for cropping systems as well as natural landscapes. Declines in both both native bee and managed honey bee (Apis mellifera L.) populations has brought attention to the significance of their role as pollinators in managed and native ecosystems. As a result, conservation efforts have been...
In cranberry (Vaccinium macrocarpon Aiton) cultivation, farmers typically rent colonies of honey bee (Apis mellifera) for pollination. However, the efficiency of this bee at pollinating cranberries in Oregon, as in other regions, is questionable. Bumble bees (Bombus sp.) are reportedly effective in other regions, but their impact in Oregon is...
The twospotted spider mite, Tetranychus urticae Koch (Acari: Tetranychidae), is a
worldwide pest of numerous agronomic and horticultural plants. Conservation
biological control of this pest can be unreliable due to asynchrony of natural enemies
and certain horticultural practices that have a direct or indirect affect on natural
enemies and the...
Bumble bees provide vital pollination services in both native and agricultural landscapes. However, in recent years, bumble bee populations have experienced global population declines. The primary causes of these declines have been attributed to the environmental impacts of pathogens, pesticide use and habitat fragmentation. While research has examined the impacts...
The importance of pollinators in native and managed landscapes is well known, and recent interest is directed towards investigating the role of native bees as providers of pollination ecosystem services. Uncertainty about bee populations at global and local scales has prompted research and general interest in conservation of bee diversity....
Black vine weevil (BVW), Otiorhynchus sulcatus Fabricius, is a serious pest in cranberry, Vaccinium macrocarpon. Larvae feeding below the soil surface cause damage to the roots and underground stems. Knowledge is sparse in regard to the damage potential of BVW in Pacific Northwest cranberry beds. Control with insecticides is limited...
Hemlock woolly adelgid, Adelges tsugae Annand, is a destructive introduced pest of hemlock trees in eastern North America, and management has focused on the use of natural enemies through classical biological control. Two species of chamaemyiid flies, Leucopis argenticollis Zetterstedt and Leucopis atrifacies (Aldrich) have been collected previously from western...
The predatory mite Typhlodromus pyri (Acari: Phytoseiidae) is the predominant species in cool climate Pacific Northwest vineyards and a principal predator of pest mites including the grapevine rust mite Calepitrimerus vitis (Acari: Eriophyidae). In recent years vineyards have been experiencing C. vitis population outbreaks leading to increased economic damage from...
Metals are a persistent form of freshwater pollution and have been shown to bioaccumulate in aquatic macroinvertebrates through direct contact with contaminated water, sediments, and through consumption of contaminated organisms. This research explored the longitudinal bioaccumulation patterns of 5 common trace metals (Cr, Cu, Zn, Cd, and Pb) in the...
The effect of anthropogenic disturbance on river systems is gaining attention, and concerns about the state of freshwater natural resources are increasing globally, as are efforts to restore habitat that has been degraded by disturbance. In rivers, non-point source pollution affects the physical characteristics of the habitat and the endemic...
Epichloë typhina (Ascomycetes: Clavicipitaceae) is an endophytic fungus that
infects perennial Pooid grasses and is the causal agent of choke disease. It is endemic
to Europe and was inadvertently introduced into orchardgrass seed production fields in
western Oregon. Choke disease, which was first recorded in Oregon in 1996, currently
infects...
Entomological evidence can be used for estimating post mortem interval (PMI). Decomposition studies have been conducted throughout the world and these studies have demonstrated that insect succession generally follows a similar pattern at a taxonomic level, specifically family, but varies at the genus and species levels with respect to geographic...
A comprehensive tribal-level classification for the world's subfamilies of Hesperiidae, the skipper butterflies, is proposed for the first time. Phylogenetic relationships between tribes and subfamilies are inferred using DNA sequence data from three gene regions: 943 bp of cytochrome oxidase subunit I (COI) (in the mitochondrial genome), 739 bp of...
Four key aspects of the relationship between predatory hoverflies and the aphid pest Brevicoryne brassicae L. on broccoli were investigated in the Willamette Valley, Oregon USA: 1) the relationship between aphid density and hoverfly oviposition, 2) the larval voracity of key hoverfly species, 3) the preferences of hoverflies and broccoli...
Many Hymenoptera, with their painful stings and noxious chemical defenses, exhibit bright aposematic warning color patterns and are the most frequently mimicked group of organisms. Such aposematic color patterns are found in parasitic wasps of the Neotropical Compsobracon group (Braconidae). Many members of this group exhibit color patterns similar to...
The black vine weevil (BVW), Otiorhynchus sulcatus (F.) (Coleoptera:
Curculionidae) is a persistent pest of nursery operations in the United States,
feeding on over 140 species of plants. The goals of this research were to 1) assess
the behavioral response of BVW to a commonly employed insecticide bifenthrin
(Talstar 0.2G®)...
Headwater streams and their riparian zones are a common, yet poorly understood,
component of Pacific Northwest landscapes. I sought to describe the ecological significance of
headwater stream riparian zones as habitat for forest-floor invertebrate communities, and to
assess how alternative management strategies for riparian zones may impact these communities.
I...
The family Bibionidae has a worldwide distribution and includes approximately 700 species in eight extant genera. Recent studies have not produced compelling evidence supporting Bibionidae as a monophyletic group or identified the sister group to bibionids. Therefore, the purpose of this study is to examine the classification and evolution of...
Invasive plants have the potential to reduce the diversity of species in plant and animal communities. I examined the negative effect of two invasive wetland plants, purple loosestrife and reed canary grass, on the species richness and diversity of plant and moth communities within 24 wetland study sites in the...
A biological inventory of the butterflies of the H. J. Andrews Experimental Forest [HJA] in Linn/Lane County, Oregon was conducted during 1994 and 1995. It was the first comprehensive survey of HJA butterflies for the site and serves as a baseline for future butterfly research. A detailed ecological account is...
Knowledge of how individual organisms behave in their environment can provide a greater understanding of population dynamics. In a predator-prey system, the choices made by predators when foraging for prey are important aspects of behavior. Particularly in the case of a stage-structured prey population, how the predator selects prey stages...
Members of the tribe Sabethini (Diptera: Culicidae) oviposit and develop in phytotelmata (plant-held waters) and are primarily distributed in a pantropical pattern. Recent cladistic studies have demonstrated that while the tribe is monophyletic, three of its 13 genera are not. The genus Wyeomyia is particularly problematic and debate among culicidologists...
This dissertation describes arthropod responses to and effects on decay processes in ponderosa pine, a dominant forest canopy tree in northern California. We used both descriptive and experimental field techniques to establish how arthropod assemblages depend on and are important to the disintegration of woody structures in forests of this...
Defensive chemical and nutritive quality of young leaves were measured for 72 individual trees of Chrysolepis [Castanopsis] chrysophylla in conjunction with light intensities of two different stand types and population numbers of Habrodais grunus herri in the Cascade mountains of Oregon. Leaves were collected in the spring and leaf characteristics...
Garden symphylans (Scutigerella immaculata Newport) are increasingly important pests of below-ground parts of over 100 crops in Oregon. The focus of our work was to improve S. immaculata management through 1) development of a bait sampling method, 2) examination of the susceptibility of selected crops to S. immaculata feeding in...
The Colorado potato beetle (CPB), Leptinotarsa decemlineata (Say), is the most devastating foliage-feeding pest of potatoes in the United States. Potential biological control agents include the nematodes Heterorhabditis marelatus Liu & Berry and Steinernema riobrave Cabanillas, Poinar & Raulston, which provided nearly 100% CPB control in previous laboratory trials. In...
The cinnabar moth, Tyria jacobaeae (L.) (Lepidoptera:Arctiidae), was released in 1959 to control the grassland weed tansy ragwort, Senecio jacobaea L. (Asteraceae), despite evidence that caterpillars of this species can feed on native plants within the genera Senecio and Packera. Previous studies confirmed the moth's ability to develop on the...
Comminution of forest leaf litter by millipedes affects litter decomposition and nutrient cycling. The millipede Harpaphe haydeniana Wood (Polydesmida: Xystodesmidae) is common in low to mid-elevation forests on the Pacific Coast of North America. In a series of experiments, the suitability of broadleaf and conifer tree litters for growth of...
Purple loosestrife is a wetland perennial that came to the eastern seaboard of
North America in ship ballast and raw wool in the 1800's. Since coming to the
United States, it has spread across the country, forming dense monospecific stands
in wetlands. Mechanical and chemical control was expensive and ineffective,...
Beetle diversity and habitat associations of five
prevalent riparian plant communities were examined along
the lower reaches of Big Beaver Creek Research Natural
Area, North Cascades National Park, Washington. These
communities were defined by dominant tree species, and
included Alder Swamps, Cedar-Hemlock Forests, Douglas-fir
Forests, Gravel Bars, and Willow-Sedge Swamps....
The seasonal biology of the obliquebanded leafroller, Choristoneura rosaceana (Harris), was studied to improve its management in Mid-Columbia area sweet cherry orchards. Overwintering OBLR larvae began to emerge from hibernacula at bud stage 2 (side green). Most overwintering larvae emerged from hibernacula within three weeks of first bud swell and...
Specialist and generalist phytoseiid mites are widely used for biological
control of spider mites. Understanding dispersal attributes of these mites is
important to implementing more stable, cost-efficient integrated pest management.
In this regard, we studied dispersal of phytoseiid mites from a local ambulatory
phase of movement within a prey patch...
Leafhoppers (Homoptera: Cicadellidae) were surveyed in Western Oregon vineyards in 1999. Four sites were sampled for leafhoppers using a sticky trap method, and 29 vineyards were sampled for leafhoppers using a sweep net method in the summer of 1999. The sticky trap and sweep net methods were chosen based on...
The effects of forest cutting on forested systems and the biotic components of those systems has been at the forefront of scientific research. The inventory and monitoring of biodiversity is one technique used for measuring the effects of forest management. Because bioinventory studies are expensive, indicator species are often sought...
Jerking, which we defined as a pronounced and often repeated lunging of the entire body, was studied in larvae among seven species of phytoseiid mites, and in the nymphal stages of Neoseiulus fallacis (Garman). This behavior was observed in all active immature stages of N. fallacis, and in larvae of...
'Golden Delicious' winter squash (GDWS), Cucurbita maxima Duchesne, provides significant amounts of pollen (24 mg) and nectar (236 μl), but with a low reward of 14% nectar sugar. The quantity of nectar produced per GDWS flower differed between sites and floral sex. The GDWS male flowers had 25% higher sugar...
The biology and behavior of the codling moth, Cydia pomonella (L.), was studied to
determine factors responsible for differences in susceptibility to moth damage among apple
and pear cultivars.
In release experiments, codling moth adults were intercepted in significantly greater
numbers in host (apple, pear) than in non-host (maple) trees,...
During summer of 1998 and 1999, 34 and 10 vineyard sites, respectively, were sampled to assess spider mite pests and associated biological control by phytoseiid mites. Vineyards studied spanned five major valleys in western Oregon where grape production occurs. Leaf samples were taken from site perimeters and centers. One leaf...
Edge effects resulting from forest fragmentation are likely to alter the distributions and
interactions of resident species. I evaluated changes in species composition, species
turnover, and relative abundance of ground arthropods across replicated transects
extending from regenerating clearcuts into old-growth Douglas-fir forests. Arthropods
were collected from 3-August to 14-September in...
The structuring of canopy arthropod communities was reviewed and investigated in relation to tree species diversity and its component factors, interspersion of different species and density of each tree species. Fifteen treatments of Douglas-fir (Pseudotsuga menziesii) and red alder (Alnus rubra) (various densities and proportions of each) were randomly assigned...
Monitoring wildlife habitats has become important to forest ecosystem management because it provides valuable information about the response of forests and their species to harvest practices, impacts from recreational use, conservation efforts, and natural and human-caused disturbances. Monitoring is a complex task that requires a variety of abiotic and biotic...
Relay strip-cropping combines two vegetation management tactics, under-sowing
and strip-management. In this study conducted from 1994 though 1997, a
cover crop seed-mixture containing oats (Avena sativa L. var. "Monida"), common
vetch (Vicia sativa L.), red clover (Trifolium pratense L.), annual ryegrass (Lolium
multflorum Lam.), and buckwheat (Fagopyrum esculentum Moench) was...
The European hazelnut, Corylus avellana L., was imported into the U.S. in the late 1800's and is now grown throughout the Willamette Valley in Oregon. A native species of hazelnut, C. cornuta Marshall, is a common shrub found in forested areas of the Pacific Northwest. Foliage of both C. avellana...
We identified and evaluated a phytoseiid predator as a biological control agent of multiple spider mites pests that occur in ornamental nurseries. When comparing species, Neoseiulus fallacis (Garman) had a wider prey range than Galendromus occidentalis (Nesbitt), a higher probability of overwintering than Neoseiulus califomicus McGregor and was equally or...
In the past 10 years we have witnessed the beginnings of the study of soil ecology as a unified science, and the general realization by soil scientists, farmers, and land managers that many of the most important economic aspects of soil health are controlled by biological factors. This research focuses...
The temperature-dependent development of pupae and adults of strawberry root weevil (SRW) Otiorhynchus ovatus (L.) was studied in strawberry (Fragaria x ananassa Duch. var. 'Totem'). The development of pupae and adults was investigated at constant temperatures from 3-33°C at 3°C intervals. Major emphasis was on the development of a weighted...
Aggregation and species association of 9 species of phytophagous and
predaceous mites were studied for 6 yr in an orchard with 100, 2 m tall 'Red
Delicious' apple trees. To quantify aggregation, a method was developed that is
powerful, and easily interpreted. It relates the proportion of habitat occupied to...
Elton (1927) realized that, intuitively at least, nature was complex and stable. And that the last property contributed to the first. This idea was challenged mathematically by Gardner and Ashby (1970) and May (1972), and in the years following various models have attempted to reconcile these opposing views. Unlike previous...
In the first part of this study we monitored the development of biological control
of purple loosestrife Lythrum salicaria over a six-year period at Morgan Lake in western
Oregon. In 1992, two beetles, Galerucella pusilla and G. calmariensis (Coleoptera:
Chrysomelidae), were released to control the wetland weed at this test...
Mosquito-borne pathogens are the cause of important, often deadly diseases
worldwide; and, use of repellents is one of the components of mosquito control programs.
The most effective mosquito repellent is N,N-dimethyl-m-toluamide, known as DEET.
Speculation on the adverse effects of DEET have been on the rise since the 1980s, and...
Several aspects affecting population trends and overwintering of Neoseiulus
fallacis (Garman) on peppermint were examined. Neoseiulus fallacis was the most
abundant phytoseiid predator mite found in peppermint surveys throughout the western U.S. Results of pesticide exclusion and cage studies showed that N. fallacis controlled Tetranychus urticae (Koch) on peppermint in...
The "old-growth controversy" in the Pacific Northwest recognized thinning as the
primary silvicultural practice for land managers to produce wildlife habitat while
continuing to produce timber. For the foreseeable future, forest stands will be harvested to
produce forest gaps and a patchwork of trees of different ages. In order to...
The adult western spotted cucumber beetle (WSCB), Diabrotica undecimpunctata
undecimpunctata Mannerheim is considered a major pest of snap beans grown for
processing in Western Oregon. Control consists of 1 or 2 insecticide sprays prior to
harvest. A commercial semiochemical-based insecticide bait, Adios TIC®, 1.3% carbaryl
+ 5% buffalo gourd root...
Pergamasus quisquiliarum Canestini is a polyphagous predatory mite that has been shown to feed on the economically important arthropod, Scutigerella immaculata Newport (Symphyla: Scutigerellidae), Collembola, Diptera larvae, Enchytraeid worms, and miscellaneous other soil organisms. This study examined the feeding behavior of P. quisquiliarum, the effects of cover cropping and tillage...
Aphids are a widespread family of plant pests, whose abilities to suppress shoot
and root growth are well documented for many terrestrial plants. Only a few studies have
been conducted on conifer aphids of the genus Cinara. Cinara pseudotsugae are known
to attack Douglas-fir seedlings, an important crop in the...
Calow (1983) realized that differences between parasites and their free-living
relatives can be explained by the differences in nutrient richness. I
propose a model that is based on Calow's idea which identifies the relative
position of different trophic strategies (e.g. predation, grazing, parasitism and
others) based on (1) the differences...
Intra- and interplant movement and dispersal of the predator mites Neoseiulus californicus (McGregor) and Neoseiulus fallacis (Garman) were studied on both lima bean (Phaseolus lunatus L.) and apple (Malus pumila Miller) branch plant systems that were seeded with excess numbers of the spider mite Tetranychus urticae Koch. Individuals of either...
Aspects concerning the bionomics of the parasitic honey bee brood mite, Varroa
jacobsoni Oudemans were investigated. Mite mortality is great during the winter season
when the host colony is not rearing brood, appearing to be independent of the mortality of
its host. In winter period, no significant association between the...
Toxicological studies were carried out to evaluate the
susceptibility of codling moth, Cydia pomonella L. to
different insecticides. Bioassay procedures for adults and
larvae were standardized in terms of posttreatment holding
and temperature, age and sex of insects, insecticide
formulations and selection of a reference strain.
This study detected a...
This work examined the importance of structural complexity of habitat, availability
of prey, and competition with ants as factors influencing the abundance and community
composition of arboreal spiders in western Oregon.
In 1993, I compared the spider communities of several host-tree species which
have different branch structure. I also assessed...
The feasibility of managing codling moth, Cydia pomonella (L.), with mixtures
of Bacillus thuringiensis-based microbial and selected chemical insecticides was
studied under laboratory and field conditions. Joint actions (synergism, additivity and
antagonism) of these mixtures were determined for both egg and larval stages. Higher
rates of microbial control agents (MCAs),...
Stream-restoration projects are usually designed to improve habitat quality for fishes.
These projects manipulate flow patterns, substrate distribution, and amount and
placement of large woody debris. Consequently, they also affect the size and composition
of the aquatic macroinvertebrate community. This research evaluates two types of fish
habitat restoration: off-channel structures...
The purpose of this research is two fold. First to improve pest management of the
sciarid fly (Diptera: Sciaridae) by better defining its relation to mushroom production.
Secondly, to explore some of the factors and aspects of the biology of the fly which may
increase room to room dispersal rates...
The genus Platylygus Van Duzee was erected in 1915 and revised by Kelton and Knight in 1970 describing 26 new species from United State and Mexico. Despite this prior revision, taxonomic problems have been encountered with the use of the keys and in the identification given the close similarity among...
The life history, temperature, humidity, and feeding requirements of the
phytoseiid predator mite, Typhlodromus americanus Chant and Yoshida-Shaul were
tested in the laboratory. Occurance of the mite in the field during different times of the
year was investigated as well. Effects of temperature and humidity on egg hatch, the
feeding...
The importance of active-entry drift at a community-scale
was evaluated by testing whether the placement of wood
in streams affected the abundance of Baetis spp. mayflies in
drift. Baetis were chosen because they use drift as a
behavioral strategy and are important in salmonid diets. The
density of woody debris...
Despite the recognized role of soil arthropod fauna on nutrient cycling
and decomposition processes, many aspects of the effects of sylvicultural
methods in forest ecosystems upon their biology remain poorly understood.
The long term effects of prescribed fires on soil arthropods in forest
ecosystems in the Pacific Northwest have never...
E8,E10-dodecadien-1-ol, the primary component of the female sex pheromone of codling moth, Cydia pomonella L., has no attraction to male Oriental fruit moth (OFM), C. molesta (Busck). However, this compound when added to the OFM pheromone blend of Z8-dodecenyl acetate (85.5%), E8-dodecenyl acetate (5.5%), and Z8- dodecen -1 -ol (9.0%),...
The recent colonization of Oregon by an exotic, intentionally introduced coccinellid, Harmonia axyridis, was the basis for lab and field research into lady
beetle developmental biology and community composition. Harmonia axyridis was the dominant coccinellid in an arboreal habitat, and comprised over 70% of all lady beetles, and 82% of...
I conducted field studies to determine the effect of insect herbivory and
intraspecific plant competition on ragwort Senecio jacobaea. The objectives were to
determine the patterns and causes in the distribution of the ragwort flea beetle
Longitarsus jacobaeae foraging among varying densities of ragwort, to measure the
behavioral and numerical...
Role of temperature and photoperiod on development of the filbert leafroller, Archips rosanus L., (Lepidoptera: Tortricidae) from the Willamette Valley, Oregon was studied in the laboratory and in the field. Both temperature and photoperiod had a significant influence on the developmental rate of this species from neonate larva to adult...
The seasonal life history of the cereal stem moth (CSM), Ochsenheimeria
vacculella Fischer von Roslerstamm, (Lepidoptera : Ochsenheimeriidae) was investigated
in a commercial field of annual ryegrass in the Willamette Valley, OR from January
1993 to August 1994. Phenology of life stages, effect of temperature on eclosion, and
development of...
Interspecific predation and cannibalism and associated rates of oviposition were
assessed for adult female Metaseiulus occidentalis (Nesbitt) and Typhlodromus pyri
Scheuten when provided non-limiting amounts of either eggs, larvae, protonymphs, or
deutonymphs. Tests with all four stages of phytoseiids and larvae of Tetranychus urticae
Koch were carried out at 25°C....
Hippodamia convergens Guerin-Meneville was selected for
rapid development through five generations at four constant
temperatures (18, 22, 26, and 30°C). Two levels of selection
were used: intense and moderate. Differences in developmental
rate, survivorship, live adult weight, aphid consumption,
adult longevity, and fecundity were measured for both groups
and an...
The probing behavior and feeding activity of green peach aphid, Myzus persicae
Sulzer, on Bacillus thuringiensis ssp. tenebrionis- transgenic potatoes, insecticide
treated potatoes, and host plants with different preference were electronically
monitored. I found that first, transgenic potatoes had no effect on green peach aphid
probing suggesting that transmission of...
This study describes and quantifies some microprocesses of malaria
transmission including blood vessel location by Anopheles stephensi and
sporozoites delivery of An. stephensi infected by Plasmodium berghei. The
study models the effects of malaria parasite-induced changes in probing
behavior and mosquito mortality on disease transmisson. Finally, host, vector,
and parasitological...
The GRASS (V. 4.0) geographical information system (GIS) was used to test
the hypothesis that pheromone trap based predictions of Helicoverpa zea (Boddie)
damage in processed sweet corn could be improved by considering spatial patterns of
corn plantings and wind blocking features around the trap. Wind blocking features
include: tree...
Honey bees mark artificial flowers with scents that
advertise about the previous history of the flower to
subsequent foragers. Unrewarding flowers are marked with a
scent, after a single visit, that makes the flower less
attractive to subsequent foragers. Previously rewarding
flowers are initially less attractive than unvisited
flowers but...
Genetics, gene flow, and distribution of pesticide
resistance traits were examined for organophosphate (OP)
resistance in three beneficial phytoseiid mites. Levels
and genetics of OP resistance in Amblyseius andersoni
were examined first. Laboratory strains from Italy and
Oregon, USA, were compared in susceptibility to
insecticides used in western Oregon fruit...
Seven streams, one of them permanent, were studied in
western Oregon, USA. The research was designed to assess
the value of summer-dry headwaters for conservation
oriented landscape management. Streams were categorized
primarily according to exposure (forest versus meadow
sites) and secondarily according to flow duration
(ephemeral = short-flow versus temporary...
Thirteen New World populations of Megachile rotundata and M. apicalis and
two Old World populations of M. rotundata were analyzed at 23 enzyme loci using
cellulose acetate electrophoresis. Conventional and G- or C-banded karyotypes were
also examined in these populations. The principal findings are (1) No differences
were found in...
The effects of natural preconditioning (source wood), wood species, and previous
exposure of wood to two types of fungi on the feeding rates and protozoa survival in the
Pacific dampwood termite Zootermopsis angusticollis (Isoptera: Termospidae) were
examined under laboratory conditions. Termites were exposed to four wood species that
had been...
Antibacterial activities in the salivary glands of
female mosquitoes Aedes aegypti were investigated in this
study.
The mean salivary bacteriolytic activity, during a
period of 14-day of female mosquitoes exposed to five
different concentrations of Gram-positive bacteria
Micrococcus lysodeikticus in the sucrose meal, was
detected by a lysoplate method. A...