The feasibility of using hollow fiber ultrafi1tration
for clarification, stabilization and preservation of white
grape juice was evaluated. Flux and process parameters of
a hollow fiber membrane ultrafi1tration unit (Romicon pilot
scale, model FXS-MXII), were studied using white Riesling
grape juice. Optimum processing conditions were determined
for different nominal membrane...
The effect of entrapped air content on heat penetration
characteristics in institutional size retortable pouches
packed with pears and green beans was investigated. In
addition, products processed in pouches and in number 10
cans were compared using several indices of quality (color,
texture, total phenolics, ascorbic acid) and sensory panel...
Edible coatings controlling preservative migration from
surface to food bulk could inhibit surface microbial growth
which is often the main cause of spoilage for many food
products. In this project we focused our attention upon
methylcellulose, hydroxypropyl methylcellulose, and chitosan as
the structural component for such edible films. These films...
The storage life at 43°F of ground dover sole fillets (Microstomas pacificus) was determined microbiologically after treatment with
ionizing radiation and antimicrobial agents. Sodium benzoate, potassium
sorbate and the sodium salts of the methyl and propyl esters
of parahydroxybenzoic acid (MPB and PPB) all approximately doubled
the refrigerated storage life...
Dimethylbenz (a) anthracene (EMBA), a carcinogen that requires
metabolic activation to produce active metabolites capable of
binding to DNA, has been studied in the trout and other fish.
Polycyclic aromatic hydrocarbons (PAH) are of importance as they
are ubiquitous in the environment and their carcinogenic effects
in fish from contaminated...
Naked barley is a rich source of fiber and phytonutrients but is not widely consumed by humans. The author contends that increasing population-wide consumption of wholegrain naked-barley would accrue a net positive public health benefit by helping to increase total fiber and phytonutrient consumption. Many of the leading causes of...
A digoxigenin-labeled nonradioactive detection system was used
to screen a zebrafish cDNA library for p53-like and ras-like genes.
One clone was isolated and identified as an incomplete p53-like
gene. The insert size of this clone is 1777 bp, which encodes part of
evolutionarily conserved region II and all of regions...
The major components of wheat flour are keys to its functionality in processing and product quality. The major components, other than the lipids, are polymers: starch, protein, and non-starchy polysaccharides (NSP). In wheat NSP are primarily arabinoxylans (AX). These components are compartmentalized in the grain but are forced into close...
Wheat is a globally traded staple crop. Wheat is important in human diets because of its agronomic adaptability, physical characteristics, functionality for the production of leavened products and nutritional value. Two significant characteristics make wheat an important staple food-crop. First, the proteins present in wheat endosperm have attributes that enable...
Fruit juice concentrates- hard pear, soft pear, fig,
prune, raisin, white grape, and pineapple, and sweeteners-invert
beet, invert cane, and high fructose corn syrup
(HFCS) were characterized by sugar profiles, nonvolatile
acid profiles, UV spectral profiles, and mineral content.
These fruit juices and sweeteners were also used to
adulterate commercial...
Mercury is a toxic heavy metal released into the environment from both natural and anthropogenic sources. The organic form of mercury is a potent neurotoxin that is especially dangerous to developing nervous systems. Results of recent studies have caused the Food and Drug Administration (FDA) and the Environmental Protection Agency...
Sensory evaluation and instrumental methods were applied to the
evaluation of strawberry juice concentrate (68°Brix) stored at
20°C which had been produced both commercially (C-SJC) and in a
pilot plant (SJC). Sensory evaluation included taste and aroma
ratings by intensity scaling and time-intensity of taste, and visual
colorimetry by matching...
The biotransformation of hop aroma via yeast is a prominent topic in the brewing industry with considerable interest placed on how yeast can enhance volatile thiol production during wort fermentation. Three potent thiols are known contributors to tropical qualities in wine and beer aroma, specifically 3-mercaptohexan-1-ol (3MH), 3-mercaptohexyl acetate (3MHA),...
Myoglobin (Mb) was extracted from Pacific sardine and added to Pacific whiting surimi to measure its effects on protein gelation. The purity of Mb extract was determined by SDS-PAGE. Mb extracted using ethanol showed higher purity than Mb extract using ammonium sulfate. The addition of 0.2% Mb significantly reduced breaking...
Honey wine, also known as mead, is one of the oldest alcoholic beverages known to man. Made primarily from honey, water, and yeast, this beverage has recently been experiencing a resurgence in popularity. It is imperative that commercial producers focus on consistently creating quality products to differentiate themselves from competitors...
Eighty-one microbial species isolated from seafoods and other marine sources were examined to determine the extent of interactions among these species. Spot-plates, cross-plates, and concurrent growth experiments at 7, 15, 20, and 30 C indicated that Lactobacillus species were capable of inhibiting other microorganisms. Lactobacillus species isolated from oysters and...
The objectives of this project were to investigate the critical factors impacting the physicochemical and antibacterial properties of β-chitosan based films derived from jumbo squid (Dosidicus gigas) pens, and to evaluate the feasibility of improving water solubility of β-chitosan through Maillard reaction. The studies examined the effect of molecular weight...
Current retail food distribution practices, microbial quality of
bakery products, and the potential for growth of food borne pathogens
(Staphylococcus aureus, Salmonella typhimurium, and Bacillus cereus)
were examined using pumpkin pie as a model.
The pumpkin pies, as obtained from the retail outlets, showed contamination
with food pathogens. The aerobic...
In an effort to understand the basis for sulfite detoxification in S. cerevisiae,
the functions of two genes were analyzed. SSU1, which encodes a plasma membrane
protein, was found to be required for efficient sulfite efflux. FZFl-4, a dominant
allele of a transcriptional activator of SSUl, was also found to...
This research investigated yeast populations and diversity during pre-fermentation cold maceration and alcoholic fermentation of Vitis vinifera L. cv. Pinot noir grapes from a commercial vineyard (Dayton, OR). Fermentations were conducted at the Oregon State University research winery in 100 L tanks while grapes from the same vineyard lot were...
Aroma is one of the major components that determines the quality of wine and consumer acceptability. Fruitiness is a highly desirable aroma quality in white wines and has been well accepted by consumers across the globe. Tropical fruit aroma has been substantially studied in Sauvignon Blanc wines due to its...
The objectives of this study were to 1) measure and compare the compositional differences of essential oil among three hop cultivars ('Centennial’, 'Citra' and 'Nelson Sauvin'), 2) identify the odor-active aroma compounds in three varieties of hops and 3) investigate the behavior of hop-derived aroma compounds in beers prepared by...
Sulfite mutants representing five complementation groups, previously derived from
an ethyl methanesulfonate-treated haploid strain of Saccharomyces cerevisiae were studied.
Although the wildtype S. cerevisiae strain used (isogenic to X2180-1 A) had a basal
tolerance for sulfite (7 μM free H₂SO₃), the sensitive and resistant mutants were found to
tolerate less...
Constituents of the wine matrix, including ethanol, affect adsorption of sulfur volatiles on solid-phase microextraction (SPME) fibers, which can impact sensitivity and accuracy of volatile sulfur analysis in wine. Several common wine sulfur volatiles, including hydrogen sulfide (H2S), methanethiol (MeSH), dimethyl sulfide (DMS), dimethyl disulfide (DMDS), dimethyl trisulfide (DMTS), diethyl...
Texture, a critical property of Asian wheat noodles, is normally assessed by
sensory evaluation. However, sensory evaluation may be impractical for wheat breeders
and noodle researchers who need to evaluate a large number of samples and have limited
sample. Instrumental Texture Profile Analysis (TPA) has been widely employed to
evaluate...
Monoterpenes are important volatile compounds to the aroma of many white wines. Many monoterpenes are chiral and found in wine as different isomers. These isomers (also known as enantiomers) are non-superimposable mirror images of each other, maintain the same molecular structure and in many instances possess different sensory characteristics. Much...
Microgard, a commercially-available pasteurized
fermented milk, was found inhibitory to gram negative
bacteria such as Pseudomonas putida, some well-known foodborne
pathogens (e.g. Salmonella, Yersinia and Aeromonas)
and some fungi as revealed by an agar incorporation assay
method. Gram positive bacteria, however, were not
inhibited. Rather, some bacteria in this group...
A novel method for isolating fish proteins by shifting pH to high acid or high alkali pH was the focus of the study. Biochemical and physicochemical properties of various pH-treated soluble fish proteins as a function of ionic strength were determined. Effect of ionic strength and various storage conditions on...
Jalapeño peppers are usually consumed raw and fresh with minimal processing. They
contain antioxidants and bioactive compounds which are of human health benefits.
There have been an increasing number of reports of foodborne outbreaks attributed to
inaccessibility of sanitizers to Salmonella , E.coli and Listeria monocytogenes when
embedded in fruit...
Pacific oysters (Crassostrea gigas), Alaska pink salmon (Oncorynchus gorbuscha) roe and chum salmon (Oncorynchus keta) fillets were treated with aqueous ozone in both pilot plant and commercial settings to determine its effect on shelf-life and microbial changes. The microbial quality was analyzed by conducting pyschrotrophic and coliform plate counts on...
Zebrafish (Brachydanio rerio) are small, freshwater teleost fishes in the family
Cyprinidae, the true minnows. They are native to the tropical latitudes of India, but
have become widespread through their use as aquarium fish and as models for several
branches of biological research. Their ease of rearing, short generation time,...
Polyphenol oxidase (PPO) from d'Anjou pears was purified and some
molecular properties were studied. The extraction of PPO was
accomplished in the presence of the phenolic binder AG 2X-8 and Triton
X-100. Chlorophyll pigment was removed by chromatography resulting in
a clear, colorless enzyme extract. Three fractions of pear PPO...
Reductive sulfate assimilation, the biological process by which sulfur-containing amino acids and key derivatives are synthesized from sulfate, is broadly shared among bacteria, fungi, and plants. It is the major, if not sole source of methionine and cysteine for Saccharomyces cerevisiae during wine fermentation. Two obligate intermediates formed in the...
Given the many significant roles that bacteria play in modern food manufacturing, it is crucial that we have a fundamental understanding of how bacterial communities assemble and behave in the food manufacturing environment. The focus of this dissertation was to investigate both the spatial and temporal distributions of microbial communities...
Historically, brewers have used dry-hopping (a cold extraction of nonvolatile and volatile chemicals from hops into fermenting or finished beer) to increase the microbial stability and shelf life of their beer. As hoppy beer styles have gained in popularity over the last decade (2007-2017), the objective of dry-hopping has turned...
The fermentation of honey-based musts has been a feature of civilization for millennia. Typically comprised of 75-90% (w/w) simple (i.e. fermentable) sugars, the glucose and fructose present are readily fermented by either pitched or adventitious micro-organisms. Nevertheless, despite its venerable heritage, honey-based fermentations are prone to inconsistency, and it is...
The growth of Brettanomyces bruxellensis is a major cause of wine spoilage due tothe production of the volatile phenols 4-ethylphenol (4-EP) and 4-ethylguaiacol (4-EG), derivatives of p-coumaric acid and ferulic acid. During the winemaking process,some microorganisms can impact the concentration of the 4-EP precursors pcoumaricand coutaric acid (tartaric acid-esterified p-coumaric...
Acidulants are an important functional ingredient group in the food industry, providing sourness, lowering pH, ensuring safe processing, and extending shelf life. Hence, understanding the effect of acids on sensory characteristics of foods is critical. Of the many topics dealing with acidulants, sourness is a topic where considerable research has...
Sensory properties of four important volatile sulfur compounds, dimethyl disulfide (DMDS), diethyl disulfide (DEDS), methanethiol (MeSH) and ethanethiol (EtSH), were determined in base Oregon Pinot noir wine in order to understand their impacts on wine aroma. Detection thresholds of these four compounds in base wine were determined first. All four...
Staling in beer is inevitable. Oxidation and increased temperatures are the main causes of stale flavors in bottled beers. Since 1934, research has been done to identify compounds and pathways involved in creating stale flavors. Although many research papers report stale flavors, there is not a lexicon per se of...
The effect of refluxing on the aromas of Valencia aqueous orange essences was
determined through analysis by GC, MS, Osme, and by a descriptive panel. The strengths
and descriptions of volatiles were investigated to determine if differences in essence aroma
character and intensity existed. During production, 1 sample was subjected...
High vegetative growth of Pinot Noir vine is a common problem in most vineyards of Oregon's Willamette Valley, where sunlight exposure and heat accumulation are limited. Consequently, growers in this region commonly use vineyard management strategies to regulate vine vigor and improve grape and wine quality. Wine quality is greatly...
Effects of high temperature aging upon certain characteristics
of bovine l. dorsi muscle were studied. Paired wholesale ribs of
carcasses were obtained subsequent to slaughter. The left rib of
each pair was held at 30°C for 24 hours, then stored at 3°C.
Analogous right ribs were immediately stored at 3°C....
The taste qualities of acidulants have not been studied in detail despite
the fact that they are widely used by the food industry. Studies on
characterizing the sensory properties of organic and inorganic acids are very
limited. Reported studies are commonly on threshold, equi-sour and the time
intensity values of...
A lexicon describing the sensory perception of carbonated water was
developed. Temporal aspects and differing ingestion conditions were
investigated for Bite and Burn sensation using time-intensity (T-I). Four CO₂
levels (0, 1.7, 2.8, and 4.6 volumes) at 3°C and 10°C were tested. Trained
panelists used a 16-pt category scale for...
The effect of washing machine separated rockfish flesh on
composition, quality and desirability was investigated.
Washing machine separated rockfish flesh resulted in a
36.88% loss of solids. The greatest reductions were found in the ash
(80.32%) and lipid (65.13%) levels in the washed flesh. Most of the
sarcoplasmic proteins were...
Two vintages of Vitis vinifera cv. Pinot noir wine from a viticulture trial evaluating nitrogen fertilization, tilling and irrigation underwent descriptive analysis using a modified version of free-choice profiling. Wines were made from three field blocks of the twelve factorial combinations of Irrigation (Dry or Irrigated), Tillage (Tilled or not...
Tropical fruit aromas are prominent in wines like Sauvignon Blanc, one of the most abundant white wines in the United States. With high consumer interest for these aromas, it has become increasingly important to understand the factors that influence the desired tropical fruit aromas in white wines. Understanding the causes...