Interspecific hybridization was initiated between wild P. acutifolius accession G40199 and P. vulgaris to introgress resistance to Acanthoscelides obtectus (bean seed weevil). F1 interspecific hybrids were recovered by embryo rescue and maintained until flowering. Mostly sterile hybrids were backcrossed twice to common bean cultivar ICA Pijao to recover sufficient fertility...
White mold, caused by Sclerotinia sclerotiorum (Lib.) de Bary, results in severe losses in the production of common bean, Phaseolus vulgaris, especially snap bean varieties. Advanced backcross QTL analysis was used to identify quantitative trait loci for resistance to white mold in an interspecific cross of P. vulgaris and P....
Purple nutsedge [Cyperus rotundas L.) has been recognized as one of the
most troublesome perennial weeds of agricultural lands in tropical and some
temperate regions. This research sought to determine the effects of timing of
herbicides, shading, and soil moisture on plant population growth and tuber
production of purple nutsedge...
A portion of this study was initiated to provide the
basis for implementing a chill unit accumulation procedure
in Yemen. Most continuous or daily minimum, maximum
temperature models severely underestimated chilling units,
and slight modifications offered little improvement for the
complex models. Only the Crossa Raynaud formula approximated
generally accepted...
Red raspberry, Rubus idaeus L., is a valuable crop for the U.S. Pacific Northwest and clonal propagation is required to produce disease-free plants and for germplasm preservation. One challenge of red raspberry micropropagation is the wide variation in growth response among the cultivars. The studies described in this dissertation were...
Legume cover crops can serve as important sources of nitrogen (N) in sustainable agriculture and can be economically beneficial when fertilizer inputs are reduced without a yield reduction. Synchronizing N mineralization from organic materials with the needs of the subsequent crop is a challenge for organic growers.
Predicting plant available...
The use of cover crops in young vineyards is not widely accepted in the Pacific Northwest due to concerns of water and nutrient competition between the cover crop and vines. The last 2 years of a 4-year study started in 2009 are reported here. The study evaluated the use of...
Cannabis sativa L. is emerging as an economically important crop, particularly for its high levels of cannabinoid production. One of these cannabinoids, cannabigerolic acid (CBGA), is the precursor to many well-researched cannabinoids such as the psychotropic delta-9-trans-tetrahydrocannabinolic acid (Δ9-THCA i.e., THCA) and the medically relevant cannabidiolic acid (CBDA). CBGA has...
Uptake and cycling of nitrogen (N) in mature trees are not well understood.
Fertilizer-N uptake, partitioning, and use-efficiency were studied in standard strain
'Topred Delicious' and spur strain 'Redspur Delicious' mature apple trees {Malus
domestica Borck) on 'Malling 7A' (M.7A) rootstocks. The treatments consisted of a
ground application in spring,...
The relationships between nitrogen (N), defoliation, and plant growth in
nursery plants were studied using almond {Prunus dulcis (Mill) D. A. Webb) trees. In
several experiments the effects of N source and availability on tree growth, N uptake,
N remobilization, and nonstructural carbohydrates (TNC) were investigated to
determine the most...
Drosophila suzukii (Diptera: Drosophilidae), is a serious pest in soft and stone fruit industries worldwide. The unique serrated ovipositor of female Drosophila suzukii allowed the species to reproduce in intact, ripening fruit before the harvest. Current control methods rely heavily on insecticide application, only becoming less profitable due to the...
Conversion of exogenous cis-zeatin to trans-zeatin in immature seeds of
Phaseolus vulgaris L. led to the isolation of a cis-trans isomerase from the endosperm.
The enzyme was purified over 2000-fold by chromatography on a series of FPLC
(anion exchange, gel filtration, and hydrophobic interaction) and Concanavalin A
columns. Non-enzymatic isomerization...
Orchard floor management (OFM) practices can improve soil function and alter
the soil biological community. Soil function is dependent on soil forming materials and
on management practices but little is known about how OFM affect orchard soil quality,
fertilizer N uptake, or tree growth and production.
Research plots were located...
Honey bees (Apis mellifera) are responsible for approximately $17 billion in crop production per year in the United States, and are arguably the most important pollinators in the nation. The future of crop pollination and production is threatened by widespread national honey bee colony losses, which have averaged approximately 30%...
Twenty-one pairs of simple sequence repeat (SSR) primers were used to
investigate genetic diversity in 270 accessions of European hazelnut (Corylus avellana)
representing a wide geographic range. A capillary electrophoresis system and ABI
Genescan® and Genotyper® software were used to determine the allele size generated
from each PCR reaction based...
Genetic and morphological characteristics of an architectural mutant in common beans were studied. The mutant had shiny, dark green leaves, overlapping leaflets, short petioles and a reproductive terminal bud even though the line did not carry the fin gene. Branching was nearly absent resulting in a single stem vine. This...
A series of experiments was designed to investigate differential
flood-tolerance and the phenomenon of flood-induced stomatal closure
of several fruit tree species. The first two experiments were conducted
with potted plants submerged outdoors 5-10 cm above the soil line.
Plant morphology, growth, leaf conductance (cL), and soil oxygen diffusion
rate...
The filbert is propagated by layerage and a faster method is
needed to keep pace with the rapidly expanding industry and the demand
for new cultivars. Propagation by seedage and graftage were the
two methods selected for evaluation in the research. Seed experiments
were initiated to identify optimum treatments for...
The Pacific Northwest region of the United States is known for quality production of blueberries, blackberries, and red raspberries. However, as the climate shifts to warmer, drier, and more extreme summers, growing these water intensive berry crops is becoming increasingly difficult. Furthermore, water regulations within agriculture are becoming more prevalent...
Organically certified Northern Highbush Blueberry (NHB; Vaccinium corymbosum L.) production in the Columbia Basin region of Oregon and Washington is expanding. Soils in the region are typically calcareous and highly alkaline, often requiring pH adjustment to an ideal range of 4.5–5.5 to support the establishment of the acid-loving, ericaceous crop....
Drought and mandatory water restrictions are limiting the availability of irrigation water in many important blueberry growing regions and new strategies are needed to maintain yield and fruit quality with less water. Three potential options for reducing water use, including deficit irrigation, irrigation cut-offs, and crop thinning, were evaluated for...
Genetic stability of micropropagated and cold-stored Fragaria x anannasa cultivar
'Pocahontas' was evaluated by Random Amplified Polymorphic DNA (RAPD) assay.
Plantlets grown for eight months on M & S medium with 5 μM and 15 μM N6-
benzyladenine (BA) were analyzed. No evidence of mutations was seen in over 200...
Pre-existing Expressed Sequence Tag (EST) libraries (1,305 sequences) and a newly constructed microsatellite-enriched genomic library (136 clones) from the V. corymbosum cultivar 'Bluecrop' were screened for unique Simple Sequence Repeat (SSR)-containing sequences. Our goal was to develop a set of robust SSR markers for use in managing the National Clonal...
Green bean flavor has been studied since the 1960’s to better understand Blue Lake flavor in processed green beans in Oregon. Research by Stevens and colleagues showed that Blue Lake flavor could be reconstituted in a bland bean by adding 0.4ppm of 1-octen-3-ol and 1.6ppm of 3-hexen-1-ol. These two volatile...
In recent years, many studies have focused on the molecular and biochemical mechanisms regulating the development of wine grapes. The course of grape berry development is directed by genetic design and is mediated by phytohormones, which regulate grape berry growth and development by orchestrating a complex network of interacting genes,...
A rootstock trial was established in 1997 at the OSU Woodhall vineyard, located in the Southern Willamette Valley, which included two experiments. The first experiment was a factorial of four varieties grafted on to 9 rootstocks, and also as ungrafted vines (a split-plot design). The second experiment was a randomized...
A rapid and sensitive HPLC method for quantifying both the
reduced and oxidized forms of glutathione simultaneously in woody
plant tissues was developed. This method was applicable to a variety
of woody plant species and different tissues. Total glutathione
content in the bark tissue of seven of the woody plant...
Hibiscus and lilacs (Syringa spp.) are economically important nursery crops in the US that accounted for 4.5 million units ($30 million) and 2 million units ($20 million), respectively (USDA, 2016). To maintain and expand the market share of ornamental plants such as lilacs and Hibiscus, novel performance, floral ornamental value,...
Experiments focusing on plant growth regulators’ concentrations and combination, mineral salt formulations and TDZ pretreatment formations were conducted to optimize in vitro shoot regeneration from leaf and petiole explants of 'Marion' blackberry. Optimum shoot formation was obtained when stock plants were incubated in TDZ pretreatment medium for three weeks before...
New highly polymorphic simple sequence repeat (SSR) markers for European hazelnut (Corylus avellana L.). were developed from several sources. The markers were characterized, and segregation in a mapping population allowed to assign them to linkage groups. Their transferability across genera and species in the Betulaceae family was investigated. SSR markers...
European hazelnut (Corylus avellana L.) is a diploid with 11 pairs of chromosomes (2n = 2x = 22), monoecious, dichogamous and wind-pollinated. Eastern filbert blight (EFB) caused by Anisogramma anomala is a serious disease of European hazelnut (Corylus avellana) in North America, and infection leads to cankers that girdle branches...
Strawberry is economically the most important berry crop grown worldwide and breeders are continuously striving to develop improved cultivars. So far, marker assisted breeding (MAB) in strawberry has been limited to the private sector. However, loci controlling some traits of economic importance such as perpetual flowering (PF) and soluble solids...