Index Catalog // ScholarsArchive@OSU

Expanding Diagnostic Assays for Pseudoloma neurophilia & Description of the Progression of Infection in Adult Zebrafish Populations

Creator:: Schuster, Corbin J.
Abstract:: Pseudoloma neurophilia is the most common pathogen reported in zebrafish (Danio rerio) research facilities and is an important threat to the zebrafish model. This microsporidian parasite can cause clinical disease, but more importantly is a causative agent of non-protocol induced variation in research. Studies utilizing infected zebrafish, could potentially confound...
Resource Type:: Dissertation
Full Text:: greatest gratitude. First, I thank my advisors Dr. Michael Kent and Dr. Justin Sanders. Mike’s enthusiasm

Physical and functional characterization of soluble protein antigen(s) produced by Renibacterium salmoninarum

Creator:: Turaga, Prasad S. D.
Abstract:: Renibacterium salmoninarum produces bacterial kidney disease (BKD) in salmonid fish. Soluble antigen(s) (SA) produced by the bacterium was characterized by physicochemical, functional and immunological methods. The molecular weight determination by SDS-PAGE analysis of SA yielded 14 different molecular weight species. Seven of these species were prominent, 57 kd protein being...
Resource Type:: Dissertation
Full Text:: blood (20%). cysteine blood agar (CBA) egg medium that contained Fryer and Sanders (1) have found

Development of monoclonal antibodies against the myxosporean, ceratomyxa shasta, and characterization of the salmonid host response

Creator:: Bartholomew, Jerri Lee
Abstract:: Monoclonal antibodies (Mabs) were produced against the myxosporean parasite, Ceratomyxa shasta, and were characterized by indirect immunofluorescent techniques, western blot analysis, and immunoelectron microscopy. The resulting Mabs reacted specifically with the trophozoite and sporoblast stages of the parasite, but not with the mature spore, indicating that certain antigens are stage-specific....
Resource Type:: Dissertation
Full Text:: patience over my years as a graduate student. Thanks to Jim Sanders, Don Stevens, Charlie Smith, and Tats

Comparison of Renibacterium salmoninarum by antigenic analysis, antimicrobial assay and growth

Creator:: Getchell, Rodman Guy
Abstract:: The antigens of eight isolates of Renibacterium salmoninarum were compared serologically using immunoelectrophoretic techniques. Seven common antigens were identified by their electrophoretic mobilities in each antigenic profile. One of these common antigens, designated Fr 21-30, was partially purified by ammonium sulfate precipitation and gel filtration. Cross adsorption analysis showed Fr...
Resource Type:: Masters Thesis
Full Text:: to Dr. J. E. Sanders for reviewing this manuscript, Dr. J. Leong for her assistance, Rich Holt for

Phylogeny and intracellular survival of Renibacterium salmoninarum

Creator:: Gutenberger, Susan K.
Resource Type:: Dissertation
Full Text:: exposure to salt water (Banner et al., 1983 and 1986; Bell, 1961; Fryer and Sanders, 1981; Sanders et al

Structural and functional analysis of the 57 kDa protein produced by the fish pathogen, Renibacterium salmoninarum

Creator:: Wiens, Gregory D.
Abstract:: Little is known about the virulence factors of Renibacterium salmoninarum, the causative agent of salmonid bacterial kidney disease. The predominant protein produced by R. salmoninarum in broth culture or during infection is a 57/58 kDa protein (p57) which is associated with strain virulence. In this study monoclonal antibodies (MAbs) to...
Resource Type:: Dissertation
Full Text:: diseases of cultured salmonids (Fryer and Sanders, 1981), and mortality due to BKD can occur in both the

Characterization of the humoral immune response to Renibacterium salmonarium in Chinook salmon Oncorhynchus tshawytscha

Creator:: Wood, Patricia A. (Patricia Ann), 1969-
Resource Type:: Masters Thesis
Full Text:: bacterial pathogens of wild and cultured salmonid fish (Fryer and Sanders 1981; Evenden et al. 1993

Antimicrobial resistance and plasmid properties of Leuconostoc and group N Streptococcus

Creator:: Orberg, Paulo K.
Abstract:: A method for rapid isolation of plasmid DNA from group N streptococci was developed. Chief advantages of the method were simplicity, the utilization of microliter quantities of reagents, and the obtainment of preparations highly enriched for covalently closed circular plasmid DNA. The method was also applied to over 15 Leuconostoc...
Resource Type:: Dissertation
Full Text:: pleasant work environment. To Judith A Scientist Doubts The practice of science is the selfless

Distribution of Ceratomyxa shasta (Myxozoa) and habitat preference of the polychaete host, Manayunkia speciosa in the Klamath River

Creator:: Stocking, Richard W.
Abstract:: Ceratomyxa shasta is a myxozoan parasite of salmonids and requires the freshwater polychaete, Manayunkia speciosa to complete its life cycle. The parasite’s distribution is currently limited to the Pacific Northwest region of North America and has been reported to cause substantial losses of both wild and hatchery salmonids. The spatial...
Resource Type:: Masters Thesis
Full Text:: ; Bartholomew et al. 1992; Sanders et al. 1970). Studies have documented as many as 29% of migrating smolts

A study of corynebacterial kidney disease of salmonid fishes

Creator:: Rowse-Eagle, Debra H.
Abstract:: This study was undertaken to compile a comprehensive review of the literature and to gain a better understanding of the host-parasite relationship in corynebacterial kidney disease of salmonid fishes. An indirect fluorescent antibody technique was developed which allowed differentiation of intra- and extracellular bacteria. This technique was used in combination...
Resource Type:: Masters Thesis
Full Text:: shorter survival and higher mortality. In further studies, Sanders et al. (1978) reported similar

ScholarsArchive@OSU

Expanding Diagnostic Assays for Pseudoloma neurophilia & Description of the Progression of Infection in Adult Zebrafish Populations

Physical and functional characterization of soluble protein antigen(s) produced by Renibacterium salmoninarum

Development of monoclonal antibodies against the myxosporean, ceratomyxa shasta, and characterization of the salmonid host response

Comparison of Renibacterium salmoninarum by antigenic analysis, antimicrobial assay and growth

Phylogeny and intracellular survival of Renibacterium salmoninarum

Structural and functional analysis of the 57 kDa protein produced by the fish pathogen, Renibacterium salmoninarum

Characterization of the humoral immune response to Renibacterium salmonarium in Chinook salmon Oncorhynchus tshawytscha

Antimicrobial resistance and plasmid properties of Leuconostoc and group N Streptococcus

Distribution of Ceratomyxa shasta (Myxozoa) and habitat preference of the polychaete host, Manayunkia speciosa in the Klamath River

A study of corynebacterial kidney disease of salmonid fishes

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