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Comparison of Flexibacter columnaris strains isolated from fish in North America and other areas of the Pacific Rim

Creator:: Song, Yen-ling
Abstract:: Twenty-two isolates of Flexibacter columnaris were collected from different species of fish from a wide geographic range. Shieh medium was selected and modified to provide satisfactory growth of F. columnaris strains. Although there were three phenotypic variations in colony morphology among the strains observed, the environmental and biochemical characteristics as...
Resource Type:: Dissertation
Full Text:: this study possible: To Dr. J. L. Fryer for the opportunity to work in his laboratory and for his

Pathogenic, antigenic, and immunogenic properties of Aeromonas salmonicida studied in juvenile coho salmon (Oncorhynchus kisutch)

Creator:: Udey, Lanny R.
Abstract:: An additional layer, exterior to the outer membrane cellwall layer, was found on Aeromonas salmonicida cells from aggregating strains. Nonaggregating strains were found to lack this layer. The nonaggregating strains were all avirulent (LD₅₀ greater than 1 X 10 ⁸ CFU/fish) while the majority of the aggregating strains were virulent...
Resource Type:: Dissertation
Full Text:: presented Typed by Esther J. Byrd for Lanny R. Udey ACKNOWLEDGEMENTS I would like extend my

Properties of anti- Aeromonas salmonicida antibodies from juvenile coho salmon (Oncorhynchus kisutch)

Creator:: Cisar, John Ormond
Abstract:: After parenteral immunization with Aeromonas salmonicida cells, the agglutination activity in juvenile coho salmon (Oncorhynchus kisutch) serum was associated with the macroglobulin serum fraction. Antibody activity was diminished by 30 minutes exposure to 50 C but not 45 C. Reduction with 2-mercaptoethanol and alkylation markedly lowered but did not completely...
Resource Type:: Dissertation
Full Text:: appreciation to the many people who contributed directly or indirectly to this investigation. To Dr. J. L

The antibody response of juvenile coho salmon (Oncorhynchus kisutch) to Aeromonas salmonicida, the causative agent of furunculosis

Creator:: Paterson, William Douglas
Abstract:: This research was initiated to investigate the effects of various parameters on the immune response of juvenile coho salmon to Aeromonas salmonicida. The antibody response and development of protective immunity in juvenile coho salmon were followed after a single intraperitoneal injection of formalin killed A. salmonicida cells emulsified in Freund's...
Resource Type:: Dissertation
Full Text:: . To Dr. J. L. Fryer for his supervision, patience and encour- agement. To the Fish Commission of

Isolation and characterization of a leukocytolytic factor from Aeromonas salmonicida

Creator:: Fuller, David Wallace
Abstract:: The purpose of this study was to find what effect temperature and immunization has on the phagocytic activity of fish leukocytes against certain disease causing bacteria. Also proposed was the isolation and characterization of a leukocytolytic factor produced by one of these bacteria, Aeromonas salmonicida. Investigations revealed that temperature had...
Resource Type:: Dissertation
Full Text:: Polyethylene glycol 6000. J. T. Baker Chemical Co., Phillipsburg, New Jersey. Culture supernatant 0.8

Certain chemical and immunological properties of the endotoxin from Vibrio anguillarum

Creator:: Abe, Patrick Masami
Abstract:: The Boivin agent from Vibrio anguillarum was extracted with the use of 0.25M trichloroacetic acid (TCA). Chemical analysis revealed that the endotoxin contained approximately 35.1% protein, 18.1% carbohydrate, 39.3% lipid, 1.8% glucosamine and evidence of ethanolamine. Sixteen different amino acids made up the protein. The aliphatic amino acid concentration was...
Resource Type:: Masters Thesis
Full Text:: Grossnicklaus for Patrick Masami Abe ACKNOWLEDGEMENT The author is deeply thankful. for Dr. J. L. Fryer's

Certain aspects of the immunology and chemotherapy of bacterial kidney disease in juvenile coho salmon (Oncorhynchus kisutch)

Creator:: Banowetz, Gary Michael
Abstract:: The detection and antigenic nature of the causative Corynebacterium of bacterial kidney disease and chemotherapy of this disease in juvenile coho salmon (Oncorhynchus kisutch) were examined. Each of 207 yearling coho salmon collected from a population undergoing a severe epizootic of bacterial kidney disease were examined for the presence of...
Resource Type:: Masters Thesis
Full Text:: people who contributed directly or indirectly to this study. To Dr. J. L. Fryer for his excellent

Oral and parenteral immunization for the control of Vibrio anguillarum, the etiological agent of vibriosis in salmonid fish

Creator:: Rohovec, J. S.
Abstract:: Efficacious vaccines were developed for the control of Vibrio anguillarum, the etiological agent of vibriosis in salmonid fish. These bacterins can be administered either orally or parenterally. It was determined that both formalin-killed lyophilized whole cells and wet-packed whole cells of the organism are effective oral immunogens. Intraperitoneal injection of...
Resource Type:: Dissertation
Full Text:: gratitude to the many people who con- tributed to this work. To Dr. J. L. Fryer for his guidance. To Dr

Development of a new vaccine delivery system for immunizing fish and investigation of the protective antigens in Vibrio anguillarum

Creator:: Gould, Rowan Wayne
Abstract:: Phenol-water and trichloroacetic acid extracts of Vibrio anguillarum were both antigenic and immunogenic when injected into coho salmon (Oncorhynchus kisutch). Cross-protection against virulent Vibrio anguillarum was observed with whole-cell bacterins and phenol-water extracts prepared from two serologically distinct groups. Gel diffusion studies indicated that intact somatic antigens were serotype specific....
Resource Type:: Dissertation
Full Text:: contributed directly or indirectly to this investigation. To Dr. J. L. Fryer for his many years of

Infection and immune response induced by Vibrio anguillarum in juvenile coho salmon (Oncorhynchus kisutch)

Creator:: Groberg, Warren J.
Abstract:: Immunity in juvenile coho salmon (Oncorhynchus kisutch) was evaluated after immunization against vibriosis by parenteral, oral or immersion methods. Relative levels of immunity were determined by protection of fish from experimental water-borne challenges with Vibrio anguillarum and by serum agglutination titers. Coho salmon eggs were exposed to bacterin during artificial...
Resource Type:: Dissertation
Full Text:: Anguillarum in Juvenile Coho Salmon (Oncorhynchus kisutch) AN ABSTRACT OF THE THESIS OF Warren "J" Groberg

ScholarsArchive@OSU

Comparison of Flexibacter columnaris strains isolated from fish in North America and other areas of the Pacific Rim

Pathogenic, antigenic, and immunogenic properties of Aeromonas salmonicida studied in juvenile coho salmon (Oncorhynchus kisutch)

Properties of anti- Aeromonas salmonicida antibodies from juvenile coho salmon (Oncorhynchus kisutch)

The antibody response of juvenile coho salmon (Oncorhynchus kisutch) to Aeromonas salmonicida, the causative agent of furunculosis

Isolation and characterization of a leukocytolytic factor from Aeromonas salmonicida

Certain chemical and immunological properties of the endotoxin from Vibrio anguillarum

Certain aspects of the immunology and chemotherapy of bacterial kidney disease in juvenile coho salmon (Oncorhynchus kisutch)

Oral and parenteral immunization for the control of Vibrio anguillarum, the etiological agent of vibriosis in salmonid fish

Development of a new vaccine delivery system for immunizing fish and investigation of the protective antigens in Vibrio anguillarum

Infection and immune response induced by Vibrio anguillarum in juvenile coho salmon (Oncorhynchus kisutch)

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