Cell populations of the marine bacterium ANT-300, from
either batch culture or continuous culture with dilution
rates ranging from D = 0.170 h⁻¹ (fast) to D = 0.015 h⁻¹ (slow) were monitored for 98 days under starvation.
Viability (CFU), acridine orange direct counts (AODC), and
optical density were measured. DNA,...
Bacterial cultures enriched from sheep rumen fluid have demonstrated the ability to detoxify pyrrolizidine alkaloids (seneciphylline and jacobine) in tansy ragwort (Senecio jacobaea). The microbes are difficult to isolate using classical anaerobic techniques, therefore, microbes from two different enrichment cultures demonstrating similar degradation activity were identified using their 16S ribosomal...
Cyclic nitramines released into all environmental compartments through anthropogenic activities are toxic and possibly carcinogenic and mutagenic. Soils on military ranges, located throughout the world in various climatic regions and close to human activities, are especially susceptible to cyclic nitramine contamination. The properties of soils on military ranges will directly...
Cyclic nitramines are a class of compounds that include most of the commonly used explosives today. These are among the most common toxicants released into the environment as a result of human activity, generated on military ranges, battlefields, and production sites. Of these, hexahydro-1,3,5-trinitro-1,3,5- triazine (RDX) is of particular interest,...
2,4,6-Trinitrotoluene (TNT) has been the common munitions used in the world and is an environmental contaminant that is amendable to reductive transformation reactions. The rumen is an extremely reductive environment containing diverse microbial populations. There are 21 pure culture ruminal bacteria species in culture collection, these were tested for the...
There have been many studies that describe the protective degradation or metabolism of potentially harmful plant toxins, such as, mimosine from Leucaena leuconcephala, pyrrolizidine alkaloids from tansy ragwort (Senecio jacobaea), oxalate and some mycotoxins by rumen microbes. There are many cases of plant-related toxicoses suffered by ruminant animals where there...
The anaerobic and highly reductive conditions found in the ovine rumen are considered favorable for the degradation of the nitroaromatic explosives such as hexahydro-1,3,5-trinitro-1,3,5-triazine (RDX). In this study, we used stable-isotope-probing to determine the bacteria responsible for the degradation of RDX in the rumen. Results indicate 10 μg mL⁻¹ (45...
Interest in the distribution of Clostridium botulinum type E
was heightened by the sudden outbreak of human botulism from
smoked whitefish chubs and canned tuna fish in 1963. The question
arose as to how widely the organism is distributed among fish
and shellfish in the Northwest and what potential hazard...
High-throughput culturing (HTC) consisting of extinction culturing in autoclaved seawater has led to the isolation and characterization of many novel strains of oligotrophic marine bacteria. Strain HTCC 2207 was isolated from the Oregon coast by the HTC method. Phylogenetic analysis based on 16S rRNA gene sequence showed that this strain...
Hatcheries are often perceived as a source of pathogen amplification, potentially increasing disease risk to free-ranging populations; at the same time, free-ranging fishes may introduce pathogens into hatcheries through untreated water sources. Many pathogens exist naturally within the environment (with the exception of introduced pathogens) and the presence of a...