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Exploring Chromodomain Genes in the Fungus Fusarium graminearum Through Targeted Genetic Manipulation

Creator:: Peters, Alec
Abstract:: DNA segments in the fungus Fusarium graminearum were identified as possible genes encoding for chromodomain proteins (CDPs), and targeted for genetic manipulation. Targeted single-gene deletion strains, as wells as GFP-S tag fusion strains, were generated from wildtype F. graminearum protoplasts, and verified strains were screened for phenotypic differences, such as...
Resource Type:: Honors College Thesis
Full Text:: Manipulation Abstract approved: Michael Freitag DNA segments in the

Exploring Chromodomain Genes in the Fungus Fusarium graminearum Through Targeted Genetic Manipulation

Creator:: Peters, Alec J.
Abstract:: DNA segments in the fungus Fusarium graminearum were identified as possible genes encoding for chromodomain proteins (CDPs), and targeted for genetic manipulation. Targeted single-gene deletion strains, as wells as GFP-S tag fusion strains, were generated from wildtype F. graminearum protoplasts, and verified strains were screened for phenotypic differences, such as...
Resource Type:: Presentation and Honors College Thesis

The role of the putative transcription factor DIS10 in PRC2-mediated gene silencing

Creator:: Jackson, Kendra K.
Abstract:: PRC2 (Polycomb Repressive Complex 2) mediated gene silencing plays an important role in cell differentiation, stem cell plasticity, and tumor suppression. In fungi, it is also involved in pathogenicity. The catalytic subunit, KMT6, trimethylates lysine 27 on histone h3 (H3K27me3) at loci targeted for silencing. In animals, PRC1 and PRC2...
Resource Type:: Honors College Thesis
Full Text:: :_____________________________________________________ Michael Freitag PRC2 (Polycomb Repressive Complex 2) mediated gene silencing plays an

A Panel of Histone H3 Mutations to Investigate Centromere Maintenance and Gene Silencing

Creator:: Esposito, Madison L.
Abstract:: Modifications of histone tail amino acid residues are necessary for many reactions involving chromatin. These processes can produce transcriptionally active, euchromatic, or inactive, heterochromatic, segments of DNA. The formation and maintenance of facultative heterochromatin is critical for the proper growth and development of many eukaryotes. Euchromatin can be changed into...
Resource Type:: Undergraduate Thesis and Presentation
Full Text:: K56Q K56R K56M K56L K64L Q68A Q68S K79Q K79R K79M K79L K122LE133A R134A F. graminearum will be used

Cheung’s Symphony No. 4 in f minor : a process of design

Creator:: Cheung, Jamie Y. Y.
Abstract:: A line of four garments was designed and developed using the various stages of the design process, based on the idea of translating music into fashion, the intangible into the tangible. The line was inspired by Tchaikovsky’s Symphony No. 4 in F Minor, guided by the composer’s music and symphonic...
Resource Type:: Honors College Thesis
Full Text:: movement also starts and ends in F Major, and lasts about nine and a half minutes. Michael Thomas of

Generation of expression plasmids to study the function of the PRC2 subunit Suz12 in Fusarium graminearum

Creator:: Park, Catherine
Abstract:: The Polycomb Repressive Complex 2 (PRC2) catalyzes genome transcription repression through the methylation of lysine residue 27 in the amino terminal region of histone 3 (H3K27me1/2/3) [2], [7]. PRC2 is made up of three highly conserved core subunits: Kmt6, Eed, and Suz12. Accurate distribution of PRC2 is essential for proper...
Resource Type:: Honors College Thesis
Full Text:: Fusarium graminearum. Abstract approved:_____________________________________________________ Michael

A Panel of Histone H3 Mutations to Investigate Centromere Maintenance and Gene Silencing

Creator:: Geisler, Mark S.
Abstract:: Histones, as part of nucleosomes, are responsible for DNA packaging in chromosomes. They also affect DNA expression by a multitude of post-translational modifications, especially prevalent on the amino terminus of histones. Co-activator protein complexes “write”, “read”, or “erase” histone modifications, and in balance determine which sections of DNA are free...
Resource Type:: Honors College Thesis
Full Text:: . Abstract approved:___________________________________________ Michael Freitag Histones, as

Deletion of gene clusters to identify novel secondary metabolites in Fusarium graminearum

Creator:: Husk, Jacob, Connolly, Lanelle, and Freitag, Michael
Abstract:: CGRB Fall Conference 2015
Resource Type:: Poster
Full Text:: Michael Freitag PmeISwaI SecMet cluster DNA Scheme 3: Capture whole clusters with plasmid marker+ 5

Modification of furfural and hydroxymethylfurfural by manganese(III)

Creator:: Morse, Lettie
Abstract:: Biomass pretreatment-derived degradation compounds, such as furfural and 5-hydroxymethyl-furfural (HMF),inhibit the growth of fermentation microorganisms that utilize biomass to produce biofuels and chemicals. Here we report that manganese(III) can degrade furfural and HMF, making them less toxic to microorganisms. Treatment with manganese(III) reduced furfural and HMF concentrations in a dose-dependent...
Resource Type:: Poster
Full Text:: . Mosier, and Michael R. Ladisch. 2011. “Soluble Inhibitors/Deactivators of Cellulase Enzymes from

Mutations of CenH3 Domains and their Effect on Chromocenter Localization and Meiosis in Neurospora crassa

Creator:: Ferrer, Sarah Theresa
Abstract:: In recent years, non-coding DNA has received much attention within the scientific community. This attention has not only illuminated the mystery behind functionally important regions like the centromere, but has also brought to light additional important questions regarding centromere mechanisms, inheritance, and cellular recognition by the kinetochore, a protein complex...
Resource Type:: Honors College Thesis
Full Text:: : __________________________________________ Michael Freitag In recent years

An evaluation of the establishment and continued effectiveness of the Galápagos National Park and Marine Reserve in Ecuador

Creator:: Asson, Danielle
Abstract:: Conservation of natural resources has become a new watchword of the scientific community. While many terrestrial ecosystems are well protected, marine ecosystems have only recently come to the forefront. Marine Protected Areas (MPAs) have been introduced as a means of protecting biodiversity, commercial stocks, and ecosystem services. There are many...
Resource Type:: Honors College Thesis
Full Text:: : . Michael Harte Conservation of natural resources has become a new watchword of the scientific

Effects of Long-term Use of Ankle Taping on Balance

Creator:: McGregor, Stephanie
Abstract:: Regular ankle taping is commonly used to prevent ankle sprains, but the effects of its long-term use are not fully understood. This study examined the effects of ankle taping on balance in athletes who regularly tape their ankles versus those who do not. Fourteen collegiate gymnasts’ ability to balance on...
Resource Type:: Honors College Thesis
Full Text:: approved: _____________________________________________ Michael Pavol

Mutation of Polycomb Repressive Complex 2 (PRC2) kmt6 to Probe in vivo Function in Fusarium

Creator:: Josephson, Brian
Abstract:: Polycomb Repressive Complex 2 (PRC2) is a histone methyltransferase that initiates facultative heterochromatin formation by trimethylating histone H3 on lysine 27 (H3K27me3). Mutations within its three primary subunits, KMT6, EED, and SUZ12, result in inherited diseases and sporadic cancers. In the fungus Fusarium graminearum, deletion of the gene encoding the...
Resource Type:: Honors College Thesis
Full Text:: . Abstract approved:_____________________________________________________ Michael Freitag

Investigation of the Effects of Commercial Salmon Fishery Management in Upper Cook Inlet, Alaska

Creator:: Fleming-Craig, Danielle
Abstract:: Optimal commercial fishery management sustains each salmon species' population while maximizing economic benefits. This thesis addresses two questions. What are the Alaska Department of Fish and Game's and the Board of Fisheries' intentions when conducting a fishery management plan in Upper Cook Inlet? How does it compare to its implementation?...
Resource Type:: Honors College Thesis
Full Text:: approved:_____________________________________________________ Michael Nelson Optimal commercial fishery

Corporate Oregon : A narrative study of Measure 97

Creator:: McMorris, Claire M.
Abstract:: Oregon Ballot Measure 97 is a contentious measure on the Oregon 2016 ballot that seeks to raise taxes on corporations with sales over 25 million dollars within the state of Oregon. Predictably, there has been a considerable amount of dialogue with the public as well as lobbying among elites to...
Resource Type:: Honors College Thesis
Full Text:: . Abstract approved:_____________________________________________________ Michael D. Jones Oregon

Perceived Credibility of News Media Sources

Creator:: Palczewski, Nicole
Abstract:: The purpose of this research is to study the relationship between popular news media sources and individual perceptions of media credibility. Findings in this research show that there is a relationship between media credibility, media habits, and media trust, which affects the choice of media that an individual determines is...
Resource Type:: Honors College Thesis
Full Text:: : ______________________________________________________ Michael Jones The purpose of this research is to study the relationship between

Sustainable US Federal Water Policy Recommendations

Creator:: Kusanto, Nicholas Christopher
Abstract:: Climate change, population growth, aging infrastructure, poor water management, and aquifer depletion are reducing US water supply. Increasing water scarcity will lead to an inability to support agriculture, industry, energy, and municipal demand that will negatively affect US economic growth and national security. Traditional water policy measures of expanding supply...
Resource Type:: Honors College Thesis
Full Text:: approved: ____________________________________________ Dr. Michael Campana Climate change

Effects of Medial and Lateral Motion of the Foot during a Forward Slip on the Loss and Recovery of Balance

Creator:: Blackwell, Allison M.
Abstract:: Falls, especially those caused by slips, are a major health concern. Past research has examined the mechanisms of preventing a fall from a forward slip; however, the effects of a mediolateral component to the slip have not been thoroughly analyzed. The purpose of this study was to examine how medial...
Resource Type:: Honors College Thesis
Full Text:: Loss and Recovery of Balance. Abstract approved: Michael Pavol Falls, especially those

Bioactivity Screening and Gene Activation Strategies in Fungi

Creator:: Coddington, Nathan C.
Abstract:: Natural products from plants, bacteria, and fungi play an important role in society. Over the last 35 years, natural products and their derivatives have inspired over 60% of all FDA approved small-molecule drugs, and are among the top prescribed therapeutics.1-2 Fleming’s discovery of the fungal antibiotic penicillin started the “Golden...
Resource Type:: Honors College Thesis
Full Text:: Michael Freitag, Committee Member, representing Biochemistry and Biophysics George F. Neuhaus

Native wildlife species (Pseudacris regilla) utilizing invasive-dominated habitat in a highly disturbed ecosystem

Creator:: Epperly, Haley K.
Abstract:: The Pacific Treefrog (Pseudacris regilla) is commonly encountered throughout the Pacific Northwest and can be an indicator for ecosystem health. At each life stage P. regilla utilizes a different habitat and therefore it is important to understand habitat use at all life stages to properly manage the ecosystem, however, few...
Resource Type:: Honors College Thesis
Full Text:: : Michael J. Adams Abstract The Pacific Treefrog (Pseudacris regilla) is commonly encountered

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