Real-time PCR assays using locked nucleic acid (LNA) probes and high resolution melt (HRM) analysis were developed for molecular differentiation of Pseudoperonospora cubensis and P. humuli, causal agents of cucurbit and hop downy mildew, respectively. The assays were based on a previously identified single nucleotide polymorphism (SNP) in the cytochrome...
To facilitate the design of Natural and Nature-Based Features (NNBF) for flood protection, this thesis expands an existing engineering design methodology to NNBF systems. The existing approach is a Level III reliability method for the performance-based design of traditional coastal engineering systems. The expanded methodology incorporates uncertainties inherent to both...
Rhizopus microsporus is a globally ubiquitous opportunistic human and plant pathogen that is known to harbor endosymbiotic bacteria. Differences between populations of clinical and environmental R. microsporus isolates have yet to be assessed on a global scale. Whole-genome sequence data were used to explore fungal biology and to assess potential...
Throughout their lifetime individuals are exposed to complex mixtures of chemicals. The study of chemical mixtures is an internationally recognized research priority, but seemingly complex challenge. To reduce the intricacy of studying mixtures, researchers have identified different prioritization methods based on exposure or the toxicity of chemicals. However, understanding the...