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Characterization of Neurospora crassa “Fatty Acid Regulator” transcription factors, FAR-1 and FAR-2, by ChIP- and RNA-sequencing

Creator:

Bredeweg, Erin L. and Freitag, Michael

Abstract:

Transcription factor (TF) genes were modified endogenously to include epitope tags for identification of TF protein binding sites by Chromatin Immunoprecipitation (ChIP), followed by high throughput sequencing. We used RNA-sequencing in carbon sources of sucrose, butyrate, and oleate in far-1, far-2, and a double far-1; far-2 mutant to find transcripts...

Resource Type:

Dataset
Transcriptome assembly and analyses of gene expression in diploid wheat Triticum monococcum

Creator:

Fox, Samuel E., Geniza, Matthew, Hanumappa, Mamatha, Naithani, Sushma, Sullivan, Chris, Preece, Justin, Tiwari, Vijay K., Elser, Justin, Leonard, Jeffery M., Sage, Abagail, Gresham, Cathy, Kerhornou, Arnaud, Bolser, Dan, McCarthy, Fiona, Kersey, Paul, Lazo, Gerard R., and Jaiswal, Pankaj

Abstract:

Background: Triticum monococcum (2n) is a close ancestor of T. urartu, the A-genome progenitor of cultivated hexaploid wheat, and is therefore a useful model for the study of components regulating photomorphogenesis in diploid wheat. In order to develop genetic and genomic resources for such a study, we constructed genome-wide transcriptomes...

Resource Type:

Dataset