Salmonid B lymphocytes were stimulated both in vivo and in vitro
and shown to produce antibody specific for the immunogen. A passive
hemolytic plaque assay was employed to demonstrate B cells producing
specific antibody. Characterization of plaque forming cells (PFCs)
involved sequential inhibition with a series of graded concentrations
of...
The in vitro effects of cortisol in regulating salmonid B cell functions were
investigated. B cells at three distinct stages of differentiation were examined for
cortisol sensitivity. B lymphocyte responses were examined 1) during the early stage
of the precursor, 2) during the intermediate stage of differentiation associated with
clonal...
The concentration of ascorbic acid in the plasma was determined on
four levels of ascorbic acid intake for seven day periods in 1946-47 and
on three levels of ascorbic acid intake for ten day periods in 1947-48.
The subjects, eight girls and eight boys, were 16 to 19 year old...
Currently little is known about the mechanisms and locations of
lymphocyte development in teleosts. In this study several aspects of the
underlying factors which govern B lymphocyte development in trout
were investigated which included: the isolation and characterization of
immunoglobulin heavy chain (IgH) genes, the recombination activating
genes 1 and...
Ascorbic acid, or vitamin C, is well known as a co-factor for proline-hydroxylase and as an anti-oxidant. However, it is also capable of forming covalent bonds, particularly in the role of a nucleophile, henceforth termed 'ascorbylation'.
The ascorbylation of electrophiles can occur under physiological conditions. Furthermore, there are a number...
Phospholipase C-β (PLC-β) isozymes are key effectors in G protein-coupled
signaling pathways. Prior research suggested that some isoforms of PLC-β may exist
and function as dimers, but little is known about dimerization of PLC-β. Data from coimmunoprecipitation
assays of differentially-tagged PLC-β constructs and sizeexclusion
chromatography of native PLC-β support homodimerization...
Phospholipase C-Jβ (PLCβ) is an important effector enzyme in G-protein-coupled
signaling pathways. Activation of PLCβ by Gα and Gβy subunits has been fairly well
characterized, but little is known about other protein interactions that may also
regulate PLCβ function. A yeast two-hybrid screen of a mouse brain cDNA library
with...
The purpose of this study of the life and career of Dr. Carl
Leonard Anderson was to recount his professional contributions to
the field of health and relate these to public health and health education. The major emphasis of this study concerned Dr. Anderson's
professional life.
The accomplishment of the...
Chronic hepatitis B disproportionately affects Asian Americans. Although Asians make up only 4.2% of the total United States (U.S.) population, they account for more than half of those infected with hepatitis B. If left untreated, chronic hepatitis B could lead to cirrhosis, liver damage, and liver cancer. Studies show that...
Vitamin C is an essential component of the human diet. Uptake of vitamin C in the cell is regulated by the function of sodium-dependent vitamin C transporters (SVCTs). SVCT1 (encoded by the SLC23A1 gene) is expressed in the colon, liver and kidney, where it plays a vital role in determining...
L. C. Dunn (1893-1974) spent most of his scientific career conducting research in
developmental genetics as a member of the Zoology Department at Columbia
University in the City of New York. He had an accomplished scientific career
researching mutations in mice, which earned him respect from other geneticists and
scientists....
Oxidative stress is the major driving force behind the aging process and many age-related diseases. However, direct experimental evidence of whether antioxidants, such as ascorbate (AA) and lipoic acid (LA) can slow the progression of aging process and/or reduce risks of developing degenerative disease is largely absent. This suggests a...
Previous studies have found that smoking may have a negative effect on
vitamin B-6 indices and have demonstrated a possible association between smoking
and depressed plasma pyridoxal-5'-phosphate (PLP) concentration. Individuals with
plasma PLP values below the adequate level of 30 nmoles/L might benefit from
consumption of vitamin B-6 supplements, but...
Although much has been written describing the
various reactions to the origin of Species in America, the Mormon reaction to Darwinism has been largely ignored. This dissertation will recount the history of this reaction as exemplified by the life and works of B. H. Roberts. Roberts's intellectual pursuits early in...
DNA microarray technology is a powerful tool for analyzing patterns in gene expression data for thousands of genes. Due to a number of systematic variations in microarray experiments, the raw gene expression data is often obfuscated by undesirable technical noises. Various normalization techniques were designed in an attempt to remove...
Bromoalkoxylation of dihydropyran with bromine and 1-borneol, followed by dehydrobromination and epoxidation, afforded trans-2-1- bornyloxy-3,4-epoxytetrahydropyran as a major product. This was treated with sodium phenylselenide to give a 1:1 mixture of (2R,3R,4R)- 2-1-bornyloxy-3-hydroxy-4-phenylselenotetrahydropyran and its (2S,3S, 4S) diastereomer. Elimination of the selenoxide, derived from oxidation of the former with hydrogen...
Amphidinolide B₁ is one of the most potent cytotoxic compounds in the
amphidinolide family. Its bioactivity and intriguing structure has drawn
attention from synthetic chemists. Although numerous synthetic efforts toward
the total synthesis of amphidinolide B₁ have been published, it still remains an
unconquered synthetic target.
One of the unique...
Spatially explicit maps of habitat relationships have proven to be valuable tools for conservation and management applications including evaluating how and which species may be impacted by large scale climate change, ongoing fragmentation of habitat, and local land-use practices. Studies have turned to remote sensing datasets as a way to...
A continuous process for the production of ceramic materials has been studied.
This method reacted metal and oxidizer in a diffusionally controlled process,
demonstrated by reacting a magnesium particle stream and hot water vapor. Many small
rich hydrogen/air diffusion flames provide an atmosphere of hot water vapor, hydrogen,
and nitrogen...
Calls for reform within engineering education frequently cite the need to better prepare the future workforce for the imminent challenges that they will face. The focus in preparing students for the workforce is often on the educational system and not on understanding the actual workplace. But if we are to...
The use of Landsat data has historically been constrained to spectral and spatial information derived from a carefully selected image or set of images. However, free and open access to Landsat imagery combined with advances in data storage and computing are revolutionizing how the Landsat temporal domain is used to...
The purpose of this study was to investigate the impact of youth
gang awareness training on school personnel. Middle and high school level
teachers, counselors, and school administrators in a rural Oregon school
district were the focus of this study.
Six specially designed modules were created by the researcher and...
Racial integration of the Louisiana public school system had a devastating
effect on its number of Black teachers. The state has yet to recover from this
reduction, as fewer Black college students pursue education degrees. This study
reports on whether or not the lack of Black educators has influenced high...
The objective of this study was to compare the effects on eating behavior,
lipids, lipoproteins, lipid peroxidation, and glycemic control in women with type 2
diabetes of a high-monounsaturated fat diet (HM) compared to a high-carbohydrate
diet (HC).
In an outpatient feeding study, ten hypertriglyceridemic postmenopausal type
2 diabetic women...
A spectral investigation of the molecular vibration region of
the potassium sulfate crystal system was undertaken. This study was
initiated to establish the feasibility of using this crystal as a
host matrix for ammonium sulfate. Since these two systems are isomorphic
and have unit cells of nearly equal volume the...
Fluid-structure interaction (FSI) is an interesting and challenging interdisciplinary area comprised of fields such as engineering- fluids/structures/solids, computational science, and mathematics. FSI has several practical engineering applications such as the design of coastal infrastructure (such as bridges, levees) subjected to harsh environments from natural forces such as tsunamis, storm surges,...
Leadership, organizational structure, and communication/interpersonal relations are key functions studied. Evaluation of the Interior Columbia Basin Ecosystem Management Project (Project) considers how well those factors were managed by Forest Service and Bureau of Land Management in a bioregional assessment during a period from November, 1994 to November, 1995. Exploration of...
Aflatoxins B₁, Q₁ and aflatoxicol, R [subscript o] F, but not B₂ were activated in the presence of NADPH by the 105,000 x g pellet from rainbow
trout (Salmo gairdneri), Mt. Shasta strain, liver to products lethal to
Bacillus subtilis GSY 1057 (metB4, hisAl, uvr-1). Electron microscopy
confirmed the microsomal...
Atlantic menhaden, compared to gulf menhaden, exhibit
a life history pattern that appears to be adapted to a more
unpredictable reproductive environment. From experiments
on laboratory-reared eggs and larvae, Atlantic menhaden have
larger eggs with larger yolk volume. They are larger at
hatching, utilize their yolk faster, begin feeding at...
The structural architecture present in marine toxin azaspiracid - 20 stereocenters, 9 rings, 3 separated spirocenters - has attracted considerable synthetic attention. Our efforts toward the synthesis of azaspiracid have led to the completion of both C₁-C₂₆ northern and C₂₇-C₄₀ southern halves. Herein, the
synthesis of southern FGHI ring system...
This investigation consisted of two parts: A. stability, B.
bioavailability of vitamin B₆ in wheat. Three variables; whole wheat
flour (WHW), white flour (W) and W enriched with vitamin B₆ (WB₆)
were tested.
Stability of vitamin B₆ during bread making and storage of
bread and flour was determined. Bread was...
The vitamin B₁₂ concentrations in both the water and sediments
of Klamath Lake were found to vary significantly during a
one-year period. The patterns of B₁₂ distribution were remarkably
similar among the three main sites. In all cases there was an extremely
sharp gradation in concentration in relation to depth...
Serine transhydroxymethylase (STHM), a pyridoxal 5'-
phosphate requiring enzyme is indirectly involved in
pyrimidine deoxynucleotide metabolism. A decrease in the
activity of this enzyme could lead to altered deoxycytidine
(dC) metabolism. This study was undertaken to determine if
a vitamin B-6 deficiency affects dC metabolism. The effect
of a vitamin...
Child care stability affects child and family outcomes. Stability reflects the time dimension of a child care arrangement. Although stability does not guarantee positive outcomes, instability appears to decrease the likelihood of achieving them. Some level of stability is a necessary, although not sufficient, characteristic of care that meets children's...
Global increases in ultraviolet-B radiation (UVBR:290-
320 nm) resulting from decreases in stratospheric ozone
concentration have the potential to alter marine primary
production and affect global climate and marine trophic
dynamics. Effects of UVBR on phytoplankton carbon fixation
were determined from open ocean exposure studies conducted
off the coast of...
The purposes of the research presented in this thesis were: (1)
to determine the precision with which it is possible to measure
changes in vitamin B6 compounds in the blood and urine following
oral doses of levels of vitamin B6 (as pyridoxine) which are in the
range of the normal...
Current dietary recommendations have placed increasing emphasis on dietary fat
as an important element to decrease risk of cardiovascular disease (CVD). Although total
fat and the fatty acid composition of diets influence the risk of CVD, the optimal amounts
of different fatty acids are not well defined, especially if n-6...
Four studies were conducted to evaluate the effect of varying levels of vitamin
B-6 (B6), protein and pyridoxine glucoside (PNG) on B6 status and requirements of
women. In the first two studies, women were fed a constant protein diet and vitamin
B-6 intakes of 0.84 to 2.39 mg/d during 10-...
Fire is the dominant disturbance process in western U.S. forests, and although effects of fire in upland forests are relatively well-studied, there is little information about fire effects on riparian forests, critical areas of the landscape for both habitat and water quality. This dissertation examines different aspects of fire effects...