Virus contamination in a potato breeding program can seriously
disrupt the variety development process. Experiments were conducted
from 1989 through 1993 to: evaluate the extent to which Potato
Leafroll Virus (PLRV) and Potato Virus Y (PVY) can invade a breeding
program, determine viral spread within hills and within individual
tubers,...
Potato Virus Y (PVY) imposes serious limitations on potato yield, quality and tuber seed
production. Traditional PVY screening requires artificial inoculation under controlled
conditions followed by ELISA to test for PVY. This method is very tedious and time
consuming thus prohibiting the screening of large segregating populations. The genes
RYadg...
Potato leafroll virus (PLRV) causes yield and quality losses in
potato. PLRV is identified by plant symptoms and serological tests
such as an enzyme-linked immunosorbent assay (ELISA). A similar
serological test, direct tissue blotting assay (DTBA), was used to
detect and monitor PLRV movement in field-inoculated Russet Burbank
plants and...
Population size of Clavibacter michiganensis subsp.
sepedonicus in potato cv Russet Burbank and plant response
as affected by drought were assessed in a greenhouse
experiment. Water deficit stress and no stress treatments,
and inoculum densities of 0 or 2 X 10⁷ cfu C. m.
sepedonicus/seed piece were arranged factorially. Stem...
A greenhouse experiment was conducted in 1991 and in
1992 to determine the effect of soil water pressure on
potato (cv Russet Burbank) plant growth and infection by
Verticillium dahliae. Soil water pressures of -0.01,
-0.03, -0.08, or -0.15 MPa were combined factorially with
two inoculum concentrations, 0 or 10...
Preliminary studies were run to determine the sensitivity and
reliability of enzyme-linked immunosorbent assay (ELISA) for the
detection of potato virus X (PVX). ELISA was found to be capable of
detecting PVX in tobacco at a 1:3333 sample dilution. The reliability
of ELISA for the detection of PVX in potato...