The Mx protein family of rainbow trout (Oncorhynchus mykiss) was characterized at the molecular level. With primers derived from a partial sequence of Perch (Perca fluviatis) Mx genomic DNA, a partial rainbow trout Mx genomic DNA sequence was obtained by polymerase chain reaction (PCR) amplification. All salmonid fish investigated contained...
Several compounds have been shown to induce the mixed function
oxidase enzyme system of animals, and also to inhibit tumor formation.
Included in this category are several vegetables and isolated
vegetable components from the Cruciferae family. In this
study rainbow trout were fed blanched freeze-dried cauliflower,
broccoli, or Brussel sprouts...
Three studies were conducted to evaluate the relationship between
insulin and nutritional parameters in rainbow trout. In the first study
effects on growth parameters and tissue composition of rainbow trout were
investigated following injection of bovine insulin at two dose levels
every 48 hr for 56 days. In addition, [¹⁴C]-leucine...
Rainbow trout were fed semi-purified diets containing cyclopropenoid
fatty acids, and the biological effects of these acids were
investigated. These fatty acids occur naturally in cottonseed oil and
have been implicated in a large number of physiological disorders.
Included in this study was an investigation of the pattern of deposition...
Three studies were conducted to determine the effects of
cyclopropenoid fatty acids (CPFA) on the membrane components of livers
of rainbow trout (Salmo gairdneri). In the first study, ¹⁴C-sterculic
acid was administered by intraperitoneal injection into rainbow trout
and the trout maintained for 72 hours. The labelled sterculic acid
was...
Biliary excretion of taurocholate in thermally acclimated rainbow trout was stimulated at higher environmental temperature (18 vs. 14 or 10° C)e Acute 4° C shifts in body temperature produced more pronounced changes in biliary excretion. Thermally modulated biliary excretion was negatively correlated with plasma half live of taurocholate. Absorption of...
Previous reports indicate the presence of multiple CYP1A sequences in rainbow
trout, but their functional differences are unknown. This report describes the cloning and partial characterization of four trout CYP1A cDNAs, which are given the tentative
designations CYP1A1v2, v3, v4, and v5. Comparison among these four and three
previously reported...
Changes in blood calcium and phosphorus concentrations were
characterized for rainbow trout (Salmo gairdneri) and coho salmon
(Oncorhynchus kisutch) following intense exercise or exposure to
suspended volcanic ash or topsoil. Significant elevations in blood
calcium and phosphorus accompanied exercise-induced acidemia in both
S. qairdneri and O. kisutch. These elevations persisted...
A dietary level of Aroclor 1254 (100 ppm) was fed to rainbow trout (Salmo gairdneri) for 15 weeks to determine the effects on hepatic microsomal enzyme induction. Fish were also fed combined polychlorinated biphenyl (PCB) (100 ppm) and cyclopropene fatty acids (CPFA) (50 ppm) to determine the effects on mixed...
A suitable method for the utilization of shrimp processing waste was investigated. The shrimp waste, which consisted of shell, viscera, and residual meat particles, was dried into a pink meal. This meal contained 5.4 percent moisture, 23.5 percent ash, 4. 1 percent crude lipid, 36. 3 percent actual protein, and...
The induction of drug-metabolizing enzymes is a
complex biochemical process manifested not only by
increases in various cytochrome P-450 isozymes and
related activities, but also by alterations in
endoplasmic reticulum membranes. Little work has been
done to examine how cytochrome P-450 inducing agents
influence other cellular processes, such as
phospholipid...