The ability of Mycobacterium sp. ELW1, a novel microbe capable of alkene oxidation, to co-metabolize phenanthrene (PHE) was studied. ELW1 was able to completely co-metabolize PHE, at different concentrations below its water solubility limit, in an aqueous environment. The alkene monooxygenases in ELW1, used to initiate oxidation of PHE, were...
Multiple drug resistance (MDR) Tuberculosis (TB), leads to increased use of “second-line” drugs; one of the most effective is ethionamide (ETA). ETA is a prodrug metabolized by a mycobacterial flavin-containing monooxygenase (EtaA) as well human flavin-containing monooxygenases (FMOs). Of the five functional FMOs of humans, FMOs 1, 2, and 3...
During the past 30 years, numerous studies have focused on the toxicities of polycyclic aromatic hydrocarbons (PAH). Laboratory and field studies have helped elucidate the detrimental effects of these chemicals on growth, reproduction and immune response. Polycyclic aromatic hydrocarbons are in the priority list of chemicals to be studied by...
Natural and synthetic estrogenic compounds detected in surface waters have
been linked to endocrine signaling disruption in several species. Endogenous
estrogen contributes to cartilage and bone deposition during development in
vertebrates, and is susceptible to perturbation from xenoestrogens. The goals of
this work were to develop a teleost model to...
More than 65,000 organic chemicals are currently in commercial production with
approximately 1000 added each year. Many chemicals are released into the environment as organic mixtures derived from complex hazardous or solid wastes. Of these, more than 1000 chemicals are of environmental concern because of their production quantities, toxicity, persistence,...
Bees provide critical pollination services to diverse agricultural crops, native plants and trees. Globally, there are reports of bee declines which have been attributed to diseases, exposure to pesticides and changes in land use that are believed to have led to a reduction in foraging resources and nesting habitats for...
The field of nanotechnology has been rapidly developing new materials. These materials have become incorporated into consumer products and the environment after only minimal assessment of their effects on human health or the environment. The toxicity of these new nanomaterials remains unknown due to the unpredictable nature of the core...
Peptide-phosphorodiamidate morpholino oligomers (PPMOs) are synthetic DNA mimics that bind and silence gene targets. Through designing PPMOs that silence essential or resistance genes in pathogens, these antisense oligomers could be utilized as novel antimicrobials. Towards this end, my thesis employed minimum inhibitory concentration assays (MICs) to identify PPMOs capable of...
Two specific goals were addressed for this dissertation. First to investigate and identify the mechanistic profile of ketoconazole (KT)-induced hepatotoxicity by utilizing in vivo and in vitro approaches determining the mechanism of action for the hepatotoxicity incurred. To date, there has not been a mechanistic determination of the hepatotoxicity associated...