Investigations of 16S rRNA gene sequences hallmark modern microbiology. These sequences provide culture-independent insight into the abundance and distribution of microbiota and serve as a principle resource through which microbial community diversity is measured. Consequently, researchers rely on 16S gene sequences to test hypotheses rooted in ecology, evolution, and disease....
A year-long bench-scale treatability study was performed to assess the feasibility of using an activated sludge sequencing batch reactor (SBR) to treat a mixture of domestic wastewater and landfill leachate. A 50/50 (v/v) of domestic wastewater and landfill leachate was treated in a 1 L SBR operated on a 12...
Background: Potato virus Y (PVY) is one of the most important plant viruses affecting potato production. The
interactions between potato and PVY are complex and the outcome of the interactions depends on the potato
genotype, the PVY strain, and the environmental conditions. A potato cultivar can induce resistance to a...
On a paced mixed-model assembly line various product variants are assembled in the same sequence of serially arranged workstations that make up the line. The goal is to assemble the products with the lowest cost possible while meeting demand requirements. Since the work content of product variants may differ at...
Bacteria are abundant in marine environments. They play important roles in nutrient cycling and form symbiotic interactions with eukaryotes. However, the vast majority of bacterial taxa are difficult to maintain in laboratory cultures, meaning that most microbiological research of the past century has focused on a small subset of bacteria....
Seed dormancy is defined as the inability of viable seeds to germinate under conditionsotherwise favorable for germination. Dormancy provides a strategy for seeds to germinateat an appropriate time. Abscisic acid (ABA) is a major hormone involved in the regulationof seed dormancy. To elucidate the molecular mechanisms of dormancy, the twoexperimental...
Organellar DNA sequences are widely used in evolutionary and population genetic studies, however, the conservative nature of chloroplast gene and genome evolution often limits phylogenetic resolution and statistical power. To gain maximal access to the historical record contained within chloroplast genomes, we have adapted multiplex sequencing-by-synthesis (MSBS) to simultaneously sequence...
We used both culture-dependent and culture-independent approaches to purify genomic DNA, and PCR to amplify 16S rRNA genes from soil sample microbes. We then sequenced the 16S rRNA amplicons of the bacterial species as well as the genomic DNA of one chosen cultured organism to identify and describe the soil...
Coral reefs are diverse ecosystems and serve many purposes including preventing erosion on coastlines and acting as a source of food, income, and culture. Acropora cervicornis, a staghorn coral, has faced a significant 80-89% decline in the Caribbean, attributed to a poorly-characterized epizootic, White Band Disease (WBD). Previous studies showed...
Few plant mitochondrial genomes have been published to date
because of their structural complexity and difficulties in their physical
isolation. Plant mitochondrial genomes are known to have a high level
of heteroplasmy and can exist as several different circular or linear
strands. Sequencing additional mitochondrial genomes will help to
understand...