Honors College Thesis
 

Sequencing the Mitochondrial Genome of the Common Milkweed: Asclepias syriaca

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https://ir.library.oregonstate.edu/concern/honors_college_theses/r494vn17x

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  • Few plant mitochondrial genomes have been published to date because of their structural complexity and difficulties in their physical isolation. Plant mitochondrial genomes are known to have a high level of heteroplasmy and can exist as several different circular or linear strands. Sequencing additional mitochondrial genomes will help to understand their highly variable structures. New sequencing technologies provide the opportunity to greatly facilitate the characterization of plant mitochondrial genomes. The mitochondrial genome of Asclepias syriaca was sequenced using high throughput Illumina technology. Reads were assembled using de novo assembly and 10 of the resulting contigs were assembled into a whole circular genome with the aid of scaffolding software and Sanger sequencing. A 687kb genome resulted and was annotated with 42 mitochondrial genes and 16 transfer RNAs. Evidence of other conformational forms of this genome were found and the in vivo structure remains unknown. This study was able to construct the whole mitochondrial genome using only Illumina and Sanger sequence data which usually isn’t possible. This may have been due to the lower level of heteroplasmy present in this individual compared to others from the same population.
  • Keywords: Asclepias syriaca, Mitochondria, Genome, Milkweed, Heteroplasmy
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