A two-part study was conducted with three poorly water soluble
drugs (phenylbutazone, tolbutamide, theophylline) to develop two new
drug delivery systems based on physical-chemical approaches.
In the first part, tolbutamide and phenylbutazone were made into
solid dispersions with various excipients using a lyophilization
technique. In vitro dissolution studies of these...
Rats given morphine-codeine combinations exhibited significantly
increased hotplate reaction times compared with rats given
an analgesic dose of one agent alone. Genera, species, sex, protein
binding and metabolism were studied as possible implicating factors
for the increased reaction times. Both male and female rats exhibited
increased reaction time, and showed...
The purpose of this study was to monitor coronary responses
occurring in the S-T segment of an electrocardiogram before, during
and after exercise following a "standard" carbohydrate-loading schedule with varied vitamin B-6 supplementation. The study was conducted
using four college-aged males who had been training a minimum of two
years...
Reported here is an investigation to determine if azinphos-methyl (AZM), an
organophosphate insecticide, adducts to hemoglobin, and if so, whether the
hemoglobin adduct could be used as a quantitative marker of occupational AZM
exposure. We hypothesized that AZM, or a metabolite of AZM, binds to hemoglobin
in erythrocytes forming an...
Indole-3-carbinol (I3C), the most abundant metabolite of glucobrassicins, is
found in cruciferous vegetables such as broccoli, Brussels sprouts, cabbage and
cauliflower. I3C has been shown to have anticarcinogenic activity against many
chemical carcinogens in multiple target organs in animal models. However, the
anticarcinogenic activity of I3C is considered indirect since...
Representing the most common flavonoid consumed in the American diet, the flavanols and their polymeric condensation products, the proanthocyanidins, are regarded as functional ingredients in various beverages, whole and processed foods, herbal remedies and supplements. Their presence in food affects food quality parameters including astringency, bitterness, sourness, sweetness, salivary viscosity,...
Wheat (Triticum aestivum) is one of the world’s major staple food crops, with flour produced from starchy endosperm being used for breads, cakes, noodles and various other wheat-based foods. The unique bread making properties of wheat are primarily attributed to its gluten-forming storage proteins: gliadins and glutenins. This study investigated...
Data were taken, following irradiation exposure to
100r and 300r cumulative dose, on 89 rabbits of three
breeds that vary greatly in body size. Hematological,
intestinal motility, and ear vascularity examinations
were performed. Least square estimates have been obtained and an analysis of variance has been conducted in
order estimate...
Newborn rats exhibit a torporous response to cold stress at 2°C
from which they revive after several hours exposure even though
respiratory, cardiovascular, and muscular movements have ceased.
At less severe temperatures (20 to 25°C) the rats respond with a non-torporous
decrease in metabolic and physiologic activity and a lowering...
In three separate trials rats were fed either a basal diet deficient
in selenium and vitamin E or the same diet supplemented with
one or both of these factors. In addition to these treatments in two
of the trials, ethoxyquin was included to provide adequate lipid antioxidant
protection and to...
In vitro studies of Mobam (4-benzo(b)thienyl N-methylcarbamate) metabolism have demonstrated that at least three ether soluble metabolites are formed in a microsomal system fortified with reduced triphosphopyridine nucleotide (NADPH). One of the metabolites was identical to 4HBT (4-hydroxy benzothiophene), the phenol of Mobam. The other two metabolites did not possess...
The effects of x-irradiation on quantitative genetic traits was
investigated by comparing the response of irradiated mice to selection
for litter size and 28-day weight with the response to selection
for the same two traits in non-irradiated lines. Irradiated lines
were maintained at three levels of x-irradiation, 25 r, 50...
In vitro studies of Mobam (4-benzo(b)thienyl N-methylcarbamate)
metabolism have demonstrated that at least three ether soluble
metabolites are formed in a microsomal system fortified with reduced
triphosphopyridine nucleotide (NADPH). One of the metabolites was
identical to 4HBT (4-hydroxy benzothiophene), the phenol of Mobam.
The other two metabolites did not possess...
An investigation of Mn uptake by five-day-old excised barley
roots revealed that a metabolically-mediated process was involved.
In short-term experiments, the rate of Mn absorption was comparable
to that of the macronutrient cations. Like other metabolically
absorbed cations, the Mn absorption rate was a direct function of
the ambient concentration...
The effects of x-irradiation on quantitative genetic traits was
investigated by comparing the response of irradiated mice to selection
for litter size and 28-day weight with the response to selection
for the same two traits in non-irradiated lines. Irradiated lines
were maintained at three levels of x-irradiation, 25 r, 50...
Four species of euryhaline teleosts (chinook salmon, Oncorhynchus
tshawytscha, Walbaum; staghorn sculpin, Leptocottus armatus,
Girard; starry flounder, Platichthys stellatus, Pallas; shiner perch
Cymatogaster aggregata, Gibbons) were exposed to x-rays. The irradiated
animals were maintained in either of two different environments.
The effects of x-radiation were determined by observing the
percent...
A combination of genetical and biochemical methods were
utilized in investigating house fly resistance to naphthalene. The
inheritance of resistance was determined by crossing a naphthalene
resistant strain of Musca domestica L. with a susceptible mutant
marker strain. Biochemical comparisons were made on substrains
isolated from the progeny of these...
A series of four trials were conducted to determine the influence
of selenium supplementation of a selenium- and vitamin E-deficient
basal diet on the absorption, excretion, tissue distribution
and placental transfer of a single dose of tritium-labeled α-tocopherol
in the rat.
In the first trial, the effect of supplementation of...
More than 2,000 male newts were collected during the summer
and maintained unfed at 10°C both pre- and post-irradiation.
Exposures ranging up to 30 kR were given with 300 kVp X-rays and
⁶⁰Co gamma rays. Half of the newts were injected (I. P.) with 15 g
DMSO/kg (the maximum sublethal...
The selenium-responsive syndromes and the discovery of
Factor 3-selenium focused attention on selenium in metabolism. Two
hypotheses have been advanced to explain the metabolic effect of
selenium: 1) substitution or sparing of alpha tocopherol, and 2) a
primary role in metabolism. The first hypothesis is supported by
Scott and Desai,...
One hundred and fifty-one weanling rats of both sexes were fed
control diets or diets containing 160 ppm diethylnitrosamine (DEN) or
150 ppm N-2-fluorenyl-acetamide (FAA). The carcinogen level was
calculated so that the LD₅₀ should be achieved at about 17 weeks.
Each carcinogen treatment was incorporated with three different diet...
The purpose of the study was to compare the visual-motor performance
of college students who reported ingestion of certain drugs with
those college students who reported no drug ingestion. The study
drugs included the following drugs or groups of drugs: tobacco,
alcohol, marijuana, LSD, mescaline, barbiturates, and amphetamines.
A total...
The effect of X-irradiation on DNA synthesis in larval salivary
glands of Drosophila hydei was studied using the technique of liquid
scintillation spectrometry and autoradiography. DNA synthesis in
salivary glands of synchronized larvae was measured at successive
stages of third instar development by in vitro uptake of ³H-thymidine
for a...
One means of evaluating the temperature requirements of an
animal is to determine changes temperature causes in the uses and
losses of energy and materials in the food the animal consumes. To
develop energy budgets for cichlids (Cichlasoma bimaculatum) at different
temperatures (20, 24, 28, 32 and 36 C) data...
The uptake, retention, and distribution of ⁶⁰Co, ⁶⁵Zn, ⁸⁵ Sr, and ¹³¹I was examined in the Rough-Skinned Newt (Taricha granulosa). Each of these radionuclides is a possible contaminant of the freshwater environment and, thus, is of importance in radioecology. The newts were maintained singly at 10° C in an unfed...
The pulmonary function scores of 250 male cigarette smokers
were compared with 406 lifetime nonsmokers. A Stead-Wells spirometer
was used to obtain forced vital capacity, forced expiratory
flow, forced mid-expiratory flow, and forced expiratory volume. All
individuals included in the sample were between. the ages of 20 and
49 years....
Bioelectric responses from the isolated eye of the purple shore
crab were measured by the electroretinogram with stimulation by
light from wavelengths of 250 to 800 nm, strontium-yttrium-90 beta
radiation, 17.5 keV x-radiation, and 3-cm microwaves.
Peak sensitivities to light stimulation were found at 350 and 500
nm. These peaks...
This experiment was designed to investigate the in vivo effects of calcium ions on tetracycline absorption when oral doses of tetracycline and calcium-containing medications were administered concurrently. Twenty-one human volunteers were selected and divided into three equal-sized groups: Group A, Group B, and Group C. Each volunteer in Group A...
The effect of concentration, vehicle and surfactant on the
absorption of benzocaine -³H across the rat's rectal mucosa was
investigated. Blood radioactivity levels were determined after the
application of benzociane -³H containing suppositories and ointments.
A wet oxidation method was used for sample digestion. Since the
radioactivity counted is the...
Tissue distribution studies of calcium and lead in brain tissue
of rats were increased subsequent to a single intravenous injection
of lead, 10 mg/kg, in the form of lead acetate. Time-course
studies demonstrated that brain concentrations of calcium and lead
(in terms of wet tissue weight) were found to be...
Injection of a 2.0 mg Cd
²⁺
/kg B.W. (I.P. as CdC1
₂
in
.05 M NaAc, pH = 6.9-7.2, .15 M or p = .15 with NaCl) resulted
in a decrease in ceruloplasmin (EC 1.12.3) p-phenylenediamine
oxidase activity within one hour of injection.
Maximal decrease (40%) occurred within six...
Forced vital capacity lung expiration examinations of nonsmoking
and cigarette-smoking male sample populations were compared to
determine if the 75 to 85 percent segment was a more sensitive test
of early lung dysfunction. The nonsmoking population was composed
of 240 men who were tested for values of forced vital capacity...
Selenium deficiency in rats results in increased carbon metabolism,
approaching twice the rate of selenium supplemented animals
(normal). This is also reflected in a 50 percent increase in lysosomal
enzyme activity in tissues of selenium deficient animals.
Isolation of rat liver lysosomes shows a 17 percent increase in
membrane thickness...
The light-adapted crayfish, Pacifastacus trowbridgii (Stimpson,
1957), displayed a behavioral response to exposure to 300 kV x-rays at
exposure rates of 10 to 30 R/s. Within this range, the proportion
of subjects that responded increased with an increase in exposure
rate. The response latency was inversely proportional to the
exposure...
The metabolism of pyrrolizidine alkaloids and other
drugs was studied with regard to nutritional status and
species differences. Pyrrolizidine alkaloids which are biotransformed
to severely toxic metabolites were isolated
from tansy ragwort (Senecio jacobaea). Semi-purified alkaloid
crystals containing five alkaloids (senecionine, seneciophylline,
jacobine, jacoline, and jacozine) were prepared
and found...
The binding of estrogen to endometrium and uterine arteries of the ewe was investigated by use of a [³H]estradiol exchange assay and by incubating whole tissue aliquants with [³ H]estradiol, a method which permits quantification of bound estrogen translocated from the cytoplasm to the nucleus. Five ewes were necropsied on...
A study was performed to assess the uptake and retention patterns
of cesium-134 in the gray-tailed vole, Microtus canicaudus Miller. To
furnish a realistic source of contaminated food for the voles, wheat
seedlings were raised in hydroponic solutions to provide radiocesium
labeled wheat clippings. The hydroponic system was designed to...
The testes of the gray-tailed vole (Microtus canicaudus Miller) exhibited a high affinity for selenium relative to other selected tissues. Uptake of selenium-75 in the testis-epididymis complex was delayed relative to uptake by the whole body, kidney, and liver. This pattern of uptake suggested that selenium was metabolically incorporated into...
This was a study designed to determine the occurrence of recovery
from radiation damage in Taricha granulosa. Irradiation with either
gamma or x-ray sources in the range of 96-10,000 rads was given. (The
mean or median survival times were determined.) Doses were fractionated
and the survival times for the same...
The ozone present in the atmosphere filters incident solar
ultraviolet (UV) radiation such that only the longer wavelengths of
the total UV spectrum emitted by the sun reach the earth's surface.
Atmospheric increases in nitrogen oxides and chlorofluoromethanes
represent a threat to the existence of this protective screen. As
the...
The primary purpose of this study was to determine the effects
of early morning and evening bouts of aerobic exercise upon the diurnal
pattern of reaction time and body temperature. An effort was
made to classify subjects according to the rhythmic trends identified
with 'morning' and 'evening' type persons.
Eight...
Roughskin newts, Taricha granulosa (Skilton), were exposed to
UV-B (280-320nm), UV-A (321-380 nm), and visible radiation from
fluorescent sunlamps (Westinghouse FS40) that were filtered with
cellulose acetate film. UV-B controls were exposed to irradiation
from sunlamps that were filtered with Mylar-D, a polyester film
that effectively absorbed UV-B radiation while...
Pharmacokinetics of theophylline and N-7 substituted
theophylline in rabbits when administered alone and in
various combinations were investigated. This thesis has
four chapters dealing with etophylline and theophylline;
proxyphylline and theophylline; dyphylline and theophylline;
and etophylline, proxyphylline and dyphylline pharmacokinetics
when administered concomitantly. All chapters
are interrelated. Each chapter has...
Purpose: The purpose of this study was to determine
the effectiveness, if any, of the P0₂ Aerobic Exerciser in
simulating high altitude training in cross country runners
living at sea level.
Procedures: This study was conducted during the 1982
winter term at Oregon State University. Four cross country
runners of...
It has been hypothesized that caffeine intake can directly interfere with estrogen synthesis; it is also well documented that caffeine can increase energy expenditure. Active women with ExMD are at risk for the female athlete triad, a syndrome associated with negative energy balance, leading to menstrual dysfunction and eventually poor...
The objective of our study was to investigate the effect of selenium on
selenoprotein W. Western blot analysis indicated that selenoprotein W is present in
muscle, brain, testis and spleen of rat tissues. Tissue distribution of selenoprotein W
was not altered in rats fed various selenium levels. Among muscle, brain,...
Recombinant human calpain-5 was expressed in insect cells using a baculovirus system. The expressed calpain-5 was purified by both traditional chromatography and by affinity-column chromatography. Both methods yielded active protease. Calpain-5 displayed very limited hydrophobicity. This indicated that calpain-5 is not a membrane binding protein. Calpain-5 had pI of 8.3....
A detailed study of iron concentrations measured by the reducible,
reactive ferrous, and extractable methods was conducted in Auke Bay,
Alaska during June 1973 and in Hood Canal, Washington during October
1973 and April 1974. The concentrations by each method were generally
highest at the bottom and decreased towards the...
Specimens of Citharichthys stigmaeus, the speckled sanddab,
were taken from Yaquina Bay, Newport, Oregon and exposed for
eight days to dissolved measured concentrations ranging from 2.9
μg/l to 190 μg/1 of the polychlorinated biphenyl, Aroclor® 1260.
Oxygen consumption was measured to determine any change from
routine levels of respiration. This...
The results of a preliminary experiment suggested that a complexed
form of cobalt was more efficacious in promoting growth of a
marine phytoplankton than ionic cobalt. The phytoplankton used in this
experiment was Coccolithus huxleyi, a vitamin B₁₂ producer, and the
cobalt complex was cobalt (II)- ethylenediaminetetracetic acid [Co(II)-
EDTA]....
The brine shrimp, Artemia, was used as an experimental
organism to study the effects of ⁶⁰Co gamma irradiation on the reproductive
performance of an animal population. The total reproductive
ability of the brine shrimp was fractionated into various components
and the effects of irradiation on each of these components
was...
Bioavailability of selenium (Se) in processed tuna and
wheat products was studied in humans and rats. The protein
source of the rat diets was torula yeast with Se supplied by
either raw, precooked or canned tuna, or whole wheat flour,
bread or bran. Sodium selenite was used as a control....
Normal human skin fibroblast cells were used to study the effect
of zinc supplementation of the media on cell growth and the competitive
binding activity of low density lipoprotein (LDL). Cells were
grown in the media containing Dulbecco's Modified-Eagle Medium (DMEM),
5% (v/v) fetal calf serum (FCS), and various levels...
In this five-week feeding study male New Zealand rabbits were
fed diets containing cyclopropene fatty acids (CPFA), diets containing
cholesterol, and diets with both CPFA and cholesterol added to test
the effect of CPFA on cholesterol metabolism and atherosclerosis
induction. When CPFA-fed animals were compared with control
animals, they tended...
There is a strong campaign underway to promote increased physical
activity among the U.S. population. The U.S. Surgeon General and the U.S.
Dietary Guidelines have presented physical activity recommendations in terms
of the amount of time accumulated in physical activity and/or in terms of
energy expenditure over the course of...
The use of heated seawater for enhancing the culture of Pacific salmon was investigated. Food consumption rate, gross food conversion efficiency, growth rate, and survival of chum (Oncorhynchus keta) and pink (O. gorbuscha} salmon fed to satiation were determined in relation to water temperature and body weight. Both species of...
Acute toxicity bioassays (96-hr TL₅₀) were employed to determine
the tolerance of coho salmon (Oncorhynchus kisutch) to inorganic
chloramines at the alevin (yolk sac fry), yolk absorption, and early
juvenile life stages. Any influence of changes in temperature, pH,
and total alkalinity on the tolerance of juvenile coho for chloramines...
Exercise increases B-vitamin (B6, B12, folate) dependent metabolic reactions, especially those related to energy production and the rebuilding and repairing of muscle tissue. These same B-vitamins are also important in maintaining low blood levels of homocysteine (Hcy), a cardiovascular disease (CVD) risk factor. Hcy levels rise when the metabolism of...
Adenosine is considered to be a neuromodulator or
cotransmitter in the periphery and CNS. This neuromodulatory
action of adenosine may be observed as an anticonvulsant effect.
Dose-response curves for R-phenylisopropyladenosine (PIA),
cyclohexyladenosine (CHA), 2-chloroadenosine (2-ClAdo), Nethylcarboxamidoadenosine
(NECA) and S-PIA were generated against
PTZ seizure thresholds in the rat. The rank...
The primary goal of my studies was to elucidate the mechanisms for the well-recognized interaction between two nutrients, vitamins E and K. The outcomes from my studies assess mechanisms for adverse effects of vitamin E and provide novel information on mechanisms for vitamin K homeostasis. These findings will provide information...
"An investigation to obtain further information on the effects of underwater explosions on flatfish and crabs was conducted offshore in coastal marine waters of Oregon on September 6 and 7, 1962. The study was conducted by the Oregon Fish Commission and financed by several companies presently engaged in oil seismic...
High hydrostatic pressure (HHP) affects the pH of weak acids due to an increased degree of acid dissociation while under pressure. The temporary pH reduction by high pressure in such a case may influence the barotolerance of microorganisms. The objective of this study was to determine the impact of weak...
The effect of 2-aminoethyl-2-thiopseudourea[superscript .]Br[superscript .]HBr (AET),
2-mercaptoethylamine[superscript .]HC1 (MEA) and trisodium calcium chelate of
diethylenetriaminepentaacetic acid (CaNa₃DTPA) on the radiosensitivity
of rat fetus was investigated. A total of 1256 fetuses were
critically examined.
Exposure of rats to 200 r whole-body X-irradiation at 9.5 days
of gestation resulted in a...
The effects of estrogen treatment on uterine weight, uterine
nucleic acid content and adrenal weight were studied. Daily doses
of 0.05μg or 0.1 μg of Estradiol 17-β were administered subcutaneously
to seven day castrated female rats for periods varying
from 0 to 168 days. After two days of treatment uterine...
White muscle disease or muscular dystrophy of sheep is a selenium
responsive malady common to many areas with an inherent natural
deficiency of selenium in the forage. The disease can be prevented in
lambs by adding trace amounts (approximately 0.1 ppm) of selenium to
the otherwise selenium deficient, forage fed...
The susceptibility of milk to copper-induced or accelerated off-flavors is well known. Other animal products, including meats have not been so extensively studied. In this study different species of animals (pigs, calves, lambs and rabbits) were fed on normal rations with and without supplements of copper sulfate. During the course...
Studies were undertaken to determine the extent and rate of
binding of Hg-203 labeled phenylmercuric acetate and mercuric ace
tate in rat kidney and liver slices and their subcellular fractions
after 37°C incubation of the slices in Krebs-Ringer -phosphate solutions
containing the mercurials at 10⁻⁴ molar. A fast and parallel...
The effects of coumestrol on the uterine weight, uterine fluid
imbibition, uterine nucleic acid content, adrenal weight and body
weight of female rats were studied. Daily doses of 20 micrograms
of coumestrol per 0.1cc propylene glycol were administered subcutaneously
to spayed rats following a seven day recovery period,
for durations...
Beginning with the discovery of x-rays by Wilhelm
Konrad Roentgen in 1895, man has had the capacity to
greatly increase radiation levels of his environment,
especially with the advent of nuclear weapons in recent
years. Radioisotopes are finding progressively wider
use in medicine, research, power production, etc., with
increased hazards...
A total of five individual experiments were conducted with
gonadectomized, male sheep to study the influence of estrogen on
the plasma level of pituitary activities, with the greatest emphasis
given to gonadotropic activity. Estrogen treatments employed were
as follows:
1. 12 mg. diethylstilbestrol (DES) implanted subcutaneously.
2. 1 mg. DES...
The effects of changes in dosimetry conditions on the radiation
sensitivity of a constant biological population were determined.
The organism used for this study was the strain B of
Escherichia coli. The percentage survivals of these cells in an
aqueous solution were determined at the same total exposure with
different...
The effects of ultraviolet (UV) irradiation on photosynthesis and
various partial reactions involving the individual photoreactions of
photosynthesis were examined. These reactions included photo-reduction in adapted green algae, Hill reaction, cyclic photophosphorylation,
the 520 nm absorbance change and variable-yield fluorescence.
The reactions were measured in spinach chloroplasts and
isolated chloroplasts...
The relationship of temperature and salinity to protein synthesis
was determined for cells of Vibrio marinus, MP-1. Protein synthesis
was measured by the incorporation of radioactive proline into
hot trichloroacetic acid precipitable material. At all salinities protein
synthesis occurred at 15°C and 20°C but not at 25°C. The critical
temperature...
The behavioral repertoire of the sea anemone, Anthopleura
xanthogrammica, was investigated to test for the occurrence of any
immediate response to various regions of the electromagnetic spectrum.
Monochromatic radiations in the visible and ultraviolet spectral
regions were produced by a 150-watt xenon lamp or a ZOO watt mercury
vapor lamp...
Particles generated from wear of prosthesis joint bearing surfaces induce inflammation and promote pathological periprosthetic osteoclast-mediated bone resorption (osteolysis). Obesity increases the risk of prosthesis failure. However, despite being morbidly obese, leptin-deficient ob/ob mice are resistant to osteolysis. These findings suggest that leptin exerts effects that act to increase particle-induced...
.......................................................................................................................... 14
Study 2.2: Effect of housing temperature on particle-induced osteolysis in WT
Study 2.3
Oxygen binding behavior and subunit association-dissociation of
the hemocyanin from Callianassa are sensitive to several environmental
variables. Callianassa californiensis is a burrowing estuarine which
must survive changes in salinity, temperature and oxygen availability.
Previous work on the structure and function of the hemocyanin was
done under conditions which did not...
This study was conducted to investigate the involvement of selenium and glutathione peroxidase in
prostaglandin biosynthesis by the ram vesicular gland.
Vesicular glands were collected from healthy intact
rams covering a wide range of selenium nutrition and were
analyzed for selenium concentrations, glutathione
peroxidase activities and levels of prostaglandin E₂...
The relative bioavailability and pharmacodynamics
of tolbutamide from two different commercially available
tablet products has been evaluated in healthy subjects
in a single dose cross over study. Products were aged
by exposing tablets to 98% relative humidity for 3 days
at ambient temperature which was found to differentially
affect both...
Cardiac A₁ adenosine receptors mediate cardioinhibitory
properties of adenosine and structural congeners. The objective of
these studies was to increase our understanding of molecular
processes involved in ligand interactions with receptors mediating
cardioinhibitory effects of adenosine and the interactions of these
receptors with guanine nucleotide binding regulatory proteins.
Porcine atrial...
Reduction of stratospheric ozone caused by human
perturbation with halocarbon compounds and the consequent
increase in mid-wavelength ultraviolet radiation (UV-B
radiation. 290-320 nm) reaching the earth's surface could
have deleterious ecological effects on marine primary
producers. Unialgal and mixed cultures of Thalassiosira
pseudonana and Thalassiosira weisflogii were exposed to
three...
Blood, liver, kidney, femur, and ovaries were assayed
from female Wistar rats following oral administration of
uranyl nitrate. Three uranium concentrations were
studied for six time periods ranging from 4 hours to 240
hours following gavage. Uranium burdens of tissues were
determined by neutron activation and delayed fission
neutron counting...