Hop, Humulus lupulus L. is a dioecious perennial species. The female hop plant produces flowers that mature into ‘cones’ which are used as a raw material in the beer brewing process to impart bitterness, flavor, and aroma. The craft beer industry in the current global beer market continues to experience...
Genetic and morphological characteristics of an architectural mutant in common beans were studied. The mutant had shiny, dark green leaves, overlapping leaflets, short petioles and a reproductive terminal bud even though the line did not carry the fin gene. Branching was nearly absent resulting in a single stem vine. This...
An apparatus and method for calibrating planar laser induced fluorescence using a two-dye, two-color method was developed. A laser sheet was expanded from a 473 nm laser to excite dye solutions into higher energy states then fluoresced through spontaneous emission. The two fluorescent dyes selected for research and development were...
The severity of wildfires around the globe is increasing. At the same time, urban development is expanding outward into areas where severe fires occur. There is an increased risk of home loss to fires in areas where severe fires and urban expansion meet. Ignition of homes or nearby fuel is...
Thymidine kinase is a key enzyme in the nucleotide salvage pathway,
catalyzing the production of dTMP from thymidine and ATP. In order to
identify the structural features of the TK protein and/or primary amino
acid sequences which contribute to the catalytic and regulatory activities of
this enzyme, an in vitro...
Background: Body Mass Index (BMI) is used as a quick and inexpensive tool to measure populations at risk for a number of metabolic and chronic conditions. In part due to its simplicity, BMI has been criticized for its inaccurate prediction concerning health outcomes. Due to the diversity in the United...
β-catenin is a multi functional protein that is involved in cell-cell adhesion and cell signaling. In non-stimulated cells, β-catenin is tightly down-regulated by GSK-3β-dependent phosphorylation at Ser and Thr residues, followed by rapid ubiquitination and proteasomal degradation. It is well established that mutations within the regulatory GSK-3β region lead to...
The persistent color (pc) trait in snap bean (Phaseolus vulgaris L.) belongs to a member of the cosmetic stay-green gene family. It is considered very desirable by snap bean breeders for its impacts on pod quality. Persistent color imparts a uniform dark-green color to pods and expresses in seeds as...
Health care providers, including complementary and alternative medical (CAM) practitioners, exert a significant influence on parental pediatric vaccination decisions. Use of CAM therapies is increasing in Oregon. Concomitantly, there has been a decade-long increase in parental vaccine refusal in Oregon, rising from 1 to 5 percent from 2000-2009. For example,...
Advances in technology such as 3D digital scanning and spatial analysis software have provided archaeologists with novel data. Specifically, these methods increase the researcher's ability to measure artifact morphology and past networks of cultural transmission, to potentially track the movement of past peoples and ideas through space and time. This...
Interspecific hybridizations between P. vulgaris and P. acutifolius
and between P. vulgaris and P. lunatus were investigated. Prefertilization
events were similar in inter- and intra-specific crosses. Fertilization
was completed in all crosses and the time of fertilization was
dependent on the maternal parent, reflecting differences in the time of
maturation...
Mutation of repetitive DNA by repeat-induced point mutation (RIP) is a process that occurs in many filamentous fungi of the Ascomycota during the sexual cycle. Concurrently, direct DNA repeats are often deleted by homologous recombination at high frequency during the sexual cycle. Thus, the processes of RIP and deletion compete...
The effects of modifying air temperature, soil temperature,
and soil moisture levels on flowering, fruiting, and chemical composition
of Tendercrop snap beans were studied in experiments in
the field and in the greenhouse during 1961, 1962, and 1963.
High maximum temperatures of 95-105° F. during bloom reduced
the percent set...
Mutation analysis is the gold standard for evaluating test-suite adequacy. It involves exhaustive seeding of all small faults in a program and evaluating the effectiveness of test suites in detecting these faults. Mutation analysis subsumes numerous structural coverage criteria, approximates fault detection capability of test suites, and the faults produced...
Maintaining genome integrity is essential for an organism, as mutation
accumulation can lead to cancer, reduced fitness, and heritable diseases in offspring.
Therefore the study of mutations, how they are induced, and how they are prevented is
vital. Biomonitor systems are useful for understanding the relevant biological effects
of a...
Declines in Greater Sage-Grouse (Centrocercus urophasianus, hereafter sage-grouse) populations could be attributed to low chick survival, which may be influenced by the availability of food and cover at sites used by females rearing broods. Habitat attributes important to broods may vary regionally; thus, it is necessary to understand factors affecting...
Noise induced hearing loss is a subject of growing
concern. Over the past 15 years, several well-documented
studies have been completed which express the relationships
between industrial noise exposure and damage to normal and
pathological ears. This research has provided knowledge of
considerable importance to developing successful hearing
conservation programs....
Mutation analysis is a well-known method for measuring the quality of test suites. However, it is computationally intensive compared to other measures, which makes it hard to use in practice. Choosing a smaller subset of mutations to run is a simple approach that can alleviate this problem. Mutation operator selection...
Mutation analysis is often used to compare the effectiveness of different test suites or testing techniques. One of the main assumptions underlying this technique is the Competent Programmer Hypothesis, which proposes that programs are very close to a correct version, or that the difference between current and correct code for...
The enzymes of dNTP synthesis in T4 infection associate to form a multienzyme complex, the T4 dNTP synthetase complex, facilitating the flow of metabolites en route to dNTPs, and their subsequent flow into DNA. Study of protein-protein interactions helps one to understand how the enzymes in the complex are organized...
This dissertation is an attempt at developing a method for the analysis and estimation of the effects of disruptions due to uncertainties. Such uncertainties may result from design changes and engineering drawing delays in large-scale, complex, research and development, or construction projects.
In order to provide management with a simple...
A time-dependent, spectral, barotropic model and a similar
two-layer primitive equation model are developed to investigate the
planetary-scale wave responses to various types of large-scale
forcing: vorticity and heat sources, and sea surface temperature (SST)
anomalies. Both models are linearized about the zonal mean states of
January climatology.
The characteristics...
The accessions used to create the Snap Bean Diversity Panel (SnAP) were 378 snap bean cultivars and germplasm lines selected from North American and European germplasm. This panel supplements the 150 member Bean CAP snap bean diversity panel (SBDP) with an additional 228 snap bean accessions. The Bean CAP SBDP...
Hepatocellular carcinoma (HCC) is currently one of the top lethal cancers with an increasing trend. Deregulation of MYC in HCC is frequently detected and always correlated with poor prognosis. As the zebrafish genome contains two differentially expressed zebrafish myc orthologs, myca and mycb, it remains unclear about the oncogenicity of...
DNA mismatch repair (MMR) is an evolutionary conserved process that functions to maintain genomic integrity through the correction of mismatches that have escaped proofreading. Mutations in the MMR gene Mlh1 are associated with approximately 50% of all cases of Lynch syndrome, a hereditary predisposition to colorectal cancer, through varying and...
Inheritance of the volatile components in bush snap beans,
O.S.U. 58-110 X 'Romano' cross and reciprocal, was determined
using gas-liquid chromatographic technique with gas-entrainment
on-column trapping. A sample of 10 to 14 pods averaging 5 grams
from a single plant was found to be adequate for F₁, F₂, and backcross...
Rice is an important crop that feeds almost half the world population. As climate change models predict floods, drought and extreme temperatures in rice production areas, the need to better understand the genetic basis of adaptation and tolerance to abiotic and biotic stresses is vital to sustain our food supply....
Published April 1975. Facts and recommendations in this publication may no longer be valid. Please look for up-to-date information in the OSU Extension Catalog: http://extension.oregonstate.edu/catalog
Published June 1960. Facts and recommendations in this publication may no longer be valid. Please look for up-to-date information in the OSU Extension Catalog: http://extension.oregonstate.edu/catalog
Genomic instability underlies diseases of unregulated cell growth that result in
cancers and developmental abnormalities in humans. Similar genome destabilizing
mechanisms are used to create genetic variety in crops for use in breeding and trait
development. Errors that occur during DNA replication may cause mutations if
they are not corrected...
Saccharomyces cerevisiae contains three NAD⁺/NADH kinases, one of which is localized in
mitochondria and phosphorylates NADH in preference to NAD⁺. Strand et al (Euk. Cell
2:809 (2003)) reported that a yeast mutation in POS5, which encodes the mitochondrial
NADH kinase, is a mutator, specific for mitochondrial genes. Because of the...
The in planta growth of three wild type strains of phytopathogenic bacteria and three nonpathogenic mutant strains was studied to determine if mutations eliminating the ability of the mutant strains to cause disease had affected their growth in leaf tissue. The mutant strains were obtained by Tn5 mutagenesis of Pseudomonas...
The easy picking characteristic, expressed as low pod detachment force (PDF),
offers a way to decrease pod damage in bush green beans mechanically harvested for
processing and fresh market, and makes hand picking easier and faster. This study was
undertaken to examine inheritance in a cross of two green bean...
An important impact of the genome technology revolution will be the elucidation of mechanisms of cancer pathogenesis, leading to improvements in the diagnosis of cancer and the selection of cancer treatment. Integrated with current well-studied massive knowledge and findings about the role of protein-coding mutations in cancer, demystifying the functional...
Though mutation analysis is the primary means of evaluating the quality of test suites, though it suffers from inadequate standardization. Mutation analysis tools vary based on language, when mutants are generated (phase of compilation), and target audience. Mutation tools rarely implement the complete set of operators proposed in the literature,...
For ras gene mutation analysis in the rainbow trout (Oncorhynchus
mykiss) model system, a partial trout ras sequence was identified
using the polymerase chain reaction (PCR). Two synthetic
oligonucleotides based on rat K-ras gene sequence were used as
primers for the PCR procedure. A 90 base pair (bp) sequence,
referred...
An impediment to use of exotic and bioengineered trees in many places is their propensity for spread by pollen and/or seeds. Our laboratory has been using gene editing to induce mutations in floral genes as means to impart stable and reliable genetic containment when this is desirable from social (markets,...
Snap beans are the vegetable form of dry bean (Phaseolus vulgaris L.) with low fiber, stringless and round, succulent pods that are eaten in the immature stage. Seed companies commit significant resources to maintaining purity and uniformity of snap bean cultivars. While some variability may be introduced through outcrossing (beans...
Integrase catalyzes insertion of a retroviral genome into the host chromosome. Following reverse transcription, integrase binds specifically to the ends of the duplex retroviral DNA, endonucleolytically cleaves two nucleotides from each 3'-end (the processing activity), and inserts these ends into the host DNA (the joining activity) in a concerted manner....
Mismatch repair (MMR) system performs mainly three roles to maintain
genomic stability, correct DNA biosynthetic errors, ensure the fidelity of
genetic recombination, and in mammalian cells participate in the cellular
response to some DNA damages. Deficiencies in mismatch repair increase
mutation rates and cancer risks. In eukaryotes, the MMR system...
To study the association between Fusarium root-rot resistance
(Fusarium solani f. sp. phaseoli) and economically inportant characteristics
of beans, 2259 lines each consisting of 30 plants were used.
The lines were randomly selected for root-rot reaction, but were
selected for differences in vigor and differences in plant and seed
pigments,...
Little is known regarding the catabolic mechanisms involved
in the breakdown of glucose in mung bean (Phaseolus aureus)
seedlings. Studies in other laboratories have shown that the TCA
cycle pathway is operative in mung bean leaves, and have demonstrated
the presence of enzymes for the oxidation of glucose to
glucuronic...
The phenomena known as "phosphorus-induced zinc deficiencies"
are poorly understood. Conclusions drawn from research efforts
designed to identify the mechanism of the phosphorus-zinc interaction
are, in many cases, incompatible. Similarities in the data from
a number of literature reports of "phosphorus-induced zinc deficiencies"
have led to the development of the...
During 2 seasons effects of high and low irrigation (water applied
at -0.6 and -2.5 bars soil water potential, respectively) and
density were evaluated on yield parameters and flower/pod development
at mainstem nodes 2 and 6 (terminal) of 'Oregon 1604', a determinate
snap bean. High and low plant densities were...
Twenty-eight bean breeding lines and cultivars were classified
for resistance to bean yellow mosaic virus type strain 7634 (BYMV)
and severe strain Gilbert 6 (BYMV-S). 'Great Northern U.I. 31'
(G.N. 31) and breeding lines (S-lines) selected from a cross between
G.N. 31 and O.S.C. 21 were chosen as parents for...
This study involved an investigation of lactic streptococci which
had irreversibly lost ability to produce the high levels of acid in milk
necessary during the manufacture of certain fermented dairy products.
The slow acid producing mutants studied were obtained from
a normal acid producing strain by natural selection following induction...
The multistage model of carcinogenesis states that an accumulation of mutations in genes that are important for maintaining cellular homeostasis may lead to cancer. A specific type of mutation observed in particular types of cancer is the CC-->TT mutation found in the TP53 gene of patients with basal or squamous...
Conversion of exogenous cis-zeatin to trans-zeatin in immature seeds of
Phaseolus vulgaris L. led to the isolation of a cis-trans isomerase from the endosperm.
The enzyme was purified over 2000-fold by chromatography on a series of FPLC
(anion exchange, gel filtration, and hydrophobic interaction) and Concanavalin A
columns. Non-enzymatic isomerization...
Field experiments were conducted in 1981 and 1982 to
develop a method for using remotely measured leaf canopy-air
temperature differences (stress-degree-days (SDD)) to
schedule irrigations and to evaluate the effects of
differential irrigation on snap bean cultivars, 'Oregon
1604' and 'Galamor'. Effects on soil water potential
(SWP), leaf water potential...
Root rot diseases of bean (Phaseolus vulgaris L.) are a problem wherever they are grown, and are a major constraint to dry edible and snap bean production. Root rot is a primary yield limitation of snap bean production in the US, especially within the top three snap bean producing states...
In a process called quorum sensing (QS), the bacterial pathogen Pseudomonas aeruginosa uses small diffusible signals to coordinate cooperative behaviors via secreted “public goods”. Under QS-dependent growth conditions, social cheaters arise with mutations in lasR, the gene for the primary QS signal receptor. These cheaters do not produce public goods....
Failure to reconstitute completely is one of the major drawbacks of dehydrated green beans. Shrivelled and flaccid beans are found in almost every reconstituted lot.
In the present study an endeavour was made to locate the chemical factors responsible for the different rehydration charac -teristics of dehydrated green beans.
Dehydrated...
Floods are the most frequent and damaging of all types of natural disasters and annually affect the lives of millions all over the globe. However, researchers seem to have overlooked the fact that floods do not recognize national boundaries. Therefore, the phenomena of shared, or transboundary floods occurring in international...
Bush snap beans (Phaseolus vulgaris L.) cvs 'G5O' (Gallatin
50) and 'OSU58' (Oregon 58) were used for anatomical and morphological
studies of their responses to three levels of temperature.
The plants were grown in a controlled chamber under a 16-hour
photoperiod at 60°/50°, 70°/60° and 80°/70°F (day/night temperature),
respectively.
The...
Experiments were performed to determine the nature of
maize influence on bean disease in additive-type
intercrops. Overall effects of intercrops on angular leaf
spot (caused by Phaeoisariopsis griseola) in Kenya
indicated >23% reductions (P<0.05) in area under the
disease progress curve (AUDPC) in two of three season-site
combinations. Fertilization tended...
Green bean flavor has been studied since the 1960’s to better understand Blue Lake flavor in processed green beans in Oregon. Research by Stevens and colleagues showed that Blue Lake flavor could be reconstituted in a bland bean by adding 0.4ppm of 1-octen-3-ol and 1.6ppm of 3-hexen-1-ol. These two volatile...
Pulsed-field gel electrophoresis (PFGE) of the strains representing the 14 races of
U. hordei, the causal agent of covered smut on barley, revealed extensive polymorphism
in chromosome length and number. The purpose of this study was to determine by two
approaches the exact chromosome number for each U. hordei race,...
Interspecific hybridization was initiated between wild P. acutifolius accession G40199 and P. vulgaris to introgress resistance to Acanthoscelides obtectus (bean seed weevil). F1 interspecific hybrids were recovered by embryo rescue and maintained until flowering. Mostly sterile hybrids were backcrossed twice to common bean cultivar ICA Pijao to recover sufficient fertility...
Summary
Recent research has identified Retinoid-X-Receptorα (RXRα), a nuclear receptor involved in ligand mediated transcription, as having a protective function against the malignant transformation of melanocytes after treatment with chemical carcinogens. This study used mice selectively lacking keratinocytic RXRα in combination with an oncogenic mutation, either Cdk4[Superscript R24C/R24C] or N-Ras[Superscript...
The advanced backcross-quatitative trait loci (AB-QTL) breeding method was employed on snap bean (OR 91G x PI433251B) and dry bean (M0162 x PI433251B) interspecific populations to transfer resistance QTL from resistant donor parent Phaseolus coccineus into P. vulgaris. For OR 91G x PI433251B, analyses produced nine linkage groups corresponding to...
Phaseolus vulgaris, the common bean, is susceptible to many
bacterial, fungal and viral diseases. However, resistance to several
diseases has been identified in related Phaseolus species, P.
coccineus, P. acutifolius and P. lunatus. Thus, interspecific
hybridization could result in transfer of beneficial characteristics.
One of the problems encountered in interspecific...
Cytokinins are a group of plant hormones mediating cell division and
differentiation. Zeatin is a naturally occurring and highly active cytokinin that is rapidly
metabolized in Phaseolus seeds. The metabolites and enzymes mediating the conversion
have been studied extensively. Zeatin metabolic enzymes may be utilized in studying the
regulation of...
Factors limiting effective extraction and purification of bean
yellow mosaic virus (BYMV) were investigated. Since the use of published
techniques for purifying BYMV resulted in low yields of partially
purified virus, an improved method for partial purification of this
virus was developed. The following treatments of infectious crude extract
decreased...
Understanding the impact of mitochondrial dysfunction on genome evolution has the potential not only to provide new insights on the basic evolutionary processes influencing mitochondrial and nuclear genomes, but may also reveal novel avenues for evolutionary adaptive recovery from harmful mutations. Aberrant mitochondrial activity is fundamental to the pathology of...
Milk, preheated at 82°C for 30 minutes, was heated to 146°C
for four seconds (UHT-treated) and cooled to 5°C in a tubular heat
exchanger. Immediately after heat treatment, 20 gallons of heated
milk were vacuum distilled at 30°C in a semi-continuous, reduced
pressure glass apparatus. Twenty gallons of non-heated milk...
Because Mexican foods have been implicated in a number of outbreaks of foodborne illness in the United States, production procedures used in Mexican restaurants were investigated by interviewing four managers. Two major problems identified through these interviews were failure to cool large quantities of beans rapidly and failure to reheat...
Benomyl-tolerant Botrytis cinerea was found in snap
bean fields throughout the Willamette Valley while no tolerant
isolates of Whetzelinia sclerotiorum were detected.
Thirty-five benomyl-tolerant isolates of B. cinerea had
slower radial growth rates than eighteen benomyl-sensitive
isolates. Sporulation of an aggressive tolerant isolate
was not stimulated when grown on benomyl-treated...
The expression and inheritance of a leaf distortion of beans was
studied in a group of the Oregon State University breeding lines and a
commercial cultivar. Of seven parents involved, two were without distortion
and classified normal, and the remainder were affected by
various degrees of distortion. The distortion affected...
In recent years, non-coding DNA has received much attention within the scientific
community. This attention has not only illuminated the mystery behind functionally
important regions like the centromere, but has also brought to light additional important
questions regarding centromere mechanisms, inheritance, and cellular recognition by the
kinetochore, a protein complex...
Published July 1956. Facts and recommendations in this publication may no longer be valid. Please look for up-to-date information in the OSU Extension Catalog: http://extension.oregonstate.edu/catalog
Sclerotinia sclerotiorum (Lib.) de Bary is a necrotrophic pathogen capable of causing white mold, a severe disease in common bean. White mold is of particular concern to the Oregon snap bean processing industry, where processors allow less than
3% incidence in harvested shipments. Breeding for white mold resistance in beans...
Cytokinin oxidase activity in callus tissues of
Phaseolus vulgaris L. cv. Great Northern has been examined
using an assay based on the oxidation of radioactively
labeled N⁶ -(Δ² -isopentenyl) adenine (i⁶Ade) to adenine
(Ade). Conditions for the quantitative extraction and
assay of the enzyme have been established. The substrate
specificity...
With respect to a multienzyme complex of deoxyribonucleoside triphosphate (dNTP) synthesis somehow juxtaposed with DNA replication sites, our laboratory has demonstrated the existence of a multienzyme complex in T4-infected E. coli, named the T4 dNTP synthetase complex, but the idea of direct linkage of dNTP synthesis to DNA replication and...
Several bean yellow mosaic virus (BYMV) strains, presumably
not transmissible by aphids, were studied to determine the biological
and environmental conditions which would possibly result in aphid
transmission of these strains. Investigations were made to study the
effect of different aphid rearing conditions on the transmission
frequency of BYMV. Also,...
Published September 1966. Facts and recommendations in this publication may no longer be valid. Please look for up-to-date information in the OSU Extension Catalog: http://extension.oregonstate.edu/catalog
The ability to establish and maintain balance is claimed to be an important prerequisite of movement tasks. However, despite this general belief, there is limited empirical evidence to support the contribution of balance in motor skill performance. Furthermore, psychometric properties of using a computerized force platform to assess balance ability...
Studies in other laboratories have established the presence of
the Embden-Meyerhof-Parnas (EMP) pathway and the pentose phosphate
(PP) pathway in plant tissue. Research in this laboratory with mung
bean (Phaseolus aureus) seedlings has confirmed the presence of these
two pathways as well as the glucuronic acid (GA) pathway. However,
no...
Esterase activity of an aqueous extract of the green bean was
separated into fourteen bands, while aqueous extracted pea esterases
revealed seven bands, by polyacrylamide-gel electrophoresis. The
fourteen bands of bean esterase activity formed three groups; slow,
intermediate and fast moving. α-Naphthyl acetate, propionate, and
n-butyrate and AS naphthol acetate...
Histones, as part of nucleosomes, are responsible for DNA packaging in chromosomes. They also affect DNA expression by a multitude of post-translational modifications, especially prevalent on the amino terminus of histones. Co-activator protein complexes “write”, “read”, or “erase” histone modifications, and in balance determine which sections of DNA are free...