This project deals with a method to optimize in vivo labeling using small fluorescent molecules via bioorthogonal reactions. The reaction used involves our unnatural amino acid 4-(6-methyl-s-tetrazin-3-yl)aminophenylalanine (AMT-Phe). The amino acid is site-specifically incorporated into GFP and then reacted with a labeled, strained trans-cyclooctene, resulting in a labeled protein. However...
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Engineering Bioorthogonal Reactions on Proteins in Live Cells
MelodieM. Machovina and Ryan A. Mehl
Labeling
This project deals with a method to optimize in vivo labeling using small fluorescent molecules via bioorthogonal reactions. The reaction used involves our unnatural amino acid 4-(6-methyl-s-tetrazin-3-yl)aminophenylalanine (AMT-Phe). The amino acid is site-specifically incorporated into GFP and then reacted with a labeled, strained trans-cyclooctene, resulting in a labeled protein. However...
Full Text:
Cells
MelodieM.
Machovina
and
Ryan
A.
Mehl
Labeling
of
biomolecules
This project deals with a method to optimize in vivo labeling using small fluorescent molecules via bioorthogonal reactions. The reaction used involves our unnatural amino acid 4-(6-methyl-s-tetrazin-3-yl)aminophenylalanine (AMT-Phe). The amino acid is site-specifically incorporated into GFP and then reacted with a labeled, strained trans-cyclooctene, resulting in a labeled protein. However...
Full Text:
.
Engineering Bioorthogonal Reactions on Proteins in Live Cells
MelodieM. Machovina and Ryan A. Mehl
Labeling
This project deals with a method to optimize in vivo labeling using small fluorescent molecules via bioorthogonal reactions. The reaction used involves our unnatural amino acid 4-(6-methyl-s-tetrazin-3-yl)aminophenylalanine (AMT-Phe). The amino acid is site-specifically incorporated into GFP and then reacted with a labeled, strained trans-cyclooctene, resulting in a labeled protein. However...
Full Text:
Cells
MelodieM.
Machovina
and
Ryan
A.
Mehl
Labeling
of
biomolecules
This project deals with a method to optimize in vivo labeling using small fluorescent molecules via bioorthogonal reactions. The reaction used involves our unnatural amino acid 4-(6-methyl-s-tetrazin-3-yl)aminophenylalanine (AMT-Phe). The amino acid is site-specifically incorporated into GFP and then reacted with a labeled, strained trans-cyclooctene, resulting in a labeled protein. However...
This project deals with a method to optimize in vivo labeling using small fluorescent molecules via bioorthogonal reactions. The reaction used involves our unnatural amino acid 4-(6-methyl-s-tetrazin-3-yl)aminophenylalanine (AMT-Phe). The amino acid is site-specifically incorporated into GFP and then reacted with a labeled, strained trans-cyclooctene, resulting in a labeled protein. However...
Rhodococcus fascians is a Gram-positive, pleiomorphic actinomycete that causes leafy gall formation in a wide range of plant hosts. This particular phytopathogen has been a focus of research at the Oregon State University Plant Clinic, a diagnostic laboratory. Phylogeny of R. fascians isolates obtained from Plant Clinic submissions was determined...
Dutch elm disease (DED) is a disease of elm trees caused by three species of Ascomycota fungi: Ophiostoma ulmi, Ophiostoma novo-ulmi, and Ophiostoma himal-ulmi. There are also two subspecies of O. novo-ulmi: subsp. americana and subsp. novo-ulmi. The pathogen is spread by bark beetles, which inhabit and traverse different elms....
Symbioses are a spectrum of interactions between organisms living in closeassociation. These intimate interactions range from mutualism, in which bothorganisms benefit, to parasitism, where one organism benefits at the expense ofthe other. Horizontal gene transfer is the acquisition of genes independent ofvertical transmission and demonstrably promotes the transition of bacteria...
The consumption of animal-sourced food products by humans is one of the most powerful negative forces affecting the conservation of terrestrial ecosystems and biological diversity. Livestock production is the single largest driver of habitat loss, and both livestock and feedstock production are increasing in developing tropical countries where the majority...
This issue is packed with new information from various research trials conducted here in Oregon, whether it is from vineyard plots, the wine lab, or the growth chamber, results are likely to have impact on vineyard and winery production here in Oregon. James Osborne, Enology Extension Specialist, opens this month’s...
Managing weak stocks in mixed-stock fisheries often relies on proxies derived from data-rich indicator stocks, although there have been limited tests of the appropriateness of such proxies. For example, full cohort reconstruction of tagged Klamath River fall-run Chinook Salmon Oncorhynchus tshawytscha of northern California enables the use of detailed models...
Members of Gram-positive Actinobacteria cause economically important diseases to plants. Within the Rhodococcus genus,
some members can cause growth deformities and persist as pathogens on a wide range of host plants. The current model
predicts that phytopathogenic isolates require a cluster of three loci present on a linear plasmid, with...
Members of Gram-positive Actinobacteria cause economically important diseases to plants. Within the Rhodococcus genus,
some members can cause growth deformities and persist as pathogens on a wide range of host plants. The current model
predicts that phytopathogenic isolates require a cluster of three loci present on a linear plasmid, with...
We report the sequence and characterization of the genomes of 21 isolates of Rhodococcus. Next generation sequencing technology was used to generate genome sequences. The reads for each genome were de novo assembled. The contigs were re-ordered, using the genome sequence of A44a as a reference. The program, Prokka, was...
We report the sequence and characterization of the genomes of 21 isolates of Rhodococcus. Next generation sequencing technology was used to generate genome sequences. The reads for each genome were de novo assembled. The contigs were re-ordered, using the genome sequence of A44a as a reference. The program, Prokka, was...
Members of Gram-positive Actinobacteria cause economically important diseases to plants. Within the Rhodococcus genus,
some members can cause growth deformities and persist as pathogens on a wide range of host plants. The current model
predicts that phytopathogenic isolates require a cluster of three loci present on a linear plasmid, with...
We report the sequence and characterization of the genomes of 21 isolates of Rhodococcus. Next generation sequencing technology was used to generate genome sequences. The reads for each genome were de novo assembled. The contigs were re-ordered, using the genome sequence of A44a as a reference. The program, Prokka, was...
The climate of the Pacific Northwest is in flux, and existing forest ecosystems are stressed and poised to shift in fundamental ways, with or without human intervention. This dissertation probes the nature of forest responses to environmental change through investigations of morphology and genetics of three species of alder co-occurring...
A necrosis at the tip of cones was observed on hop (Humulus lupulus), cultivar "Nugget", grown in Oregon in the early 1990's. Fusarium sambucinum and F. avenaceum were recovered from symptomatic cones in 1998 and preliminary inoculation experiments suggested both Fusarium species could cause hop cone necrosis. Studies were carried...
Passage through The Vagina Monologues: A College Anti-Violence Rite examines the ways in which the audience at Oregon State University (OSU) responds to the annual production of the play in connection with the international college campaign to raise awareness about violence against women and to raise money for community organizations...
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________________________________________________________________________
Susan M. Shaw
Passage through The Vagina Monologues: A College Anti-Violence Rite
Standard accounts of women's relationship with technology stress women's need to overcome anxiety to achieve competence with computers. Recent studies provide evidence that this woman-anxiety-technology connection is an oversimplification of the relationship between women and computers. New literature also suggests that making computers more appealing will help girls overcome computational...
Immobilized proteins are integral components of a number of important technologies, including the principal component of many biosensors, as functional coatings in various implants, as catalysts is industrial biosynthesis, and as the building blocks of next-generation nanotechnologies. Despite their ubiquity, relatively little is known about the processes that shape immobilized...
The Willamette Valley, west of the Cascade mountain range in the state of Oregon, is home to a growing number of vineyards. This rather recent, booming industry of the region has become an important component of the economy of the state’s agriculture since the late 1960’s, contributing more than $1.56...
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Melodie Putnam 2008, Oregon State University
1Lee, J. and Martin, R.R. (2009) Influence of
Despite the advance of biomedical science, infectious diseases remain one of the number one killers in the world. They claim the lives of millions of people annually, particularly in underdeveloped and developing countries. In fact, more than 32% of the health burden in Africa is caused by infectious diseases, e.g.,...
The inverse electron demand Diels Alder reaction between tetrazines and strained alkenes is an exceptionally useful tool in functionalizing to biomolecules since it is orthogonal to the chemistry of most living systems and have exceptionally high rate constants. In particular reactions between strained trans-cyclooctenes (sTCO) and tetrazines can achieve second...
Horticultural and nursery crops are the leading agricultural commodity in Oregon. Among currently grown nursery plants, Verbena x hybrida is a staple ornamental. It is grown and shipped worldwide, thus requiring a high level of certainty concerning the plants’ health and potential viability. V. x hybrida cultivars have been infected...
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, 2004.
APPROVED:
Mentor: Dr. Robert R. Martin
Committee Member: Melodie L. Putnam
Bioresource
This publication is offered as a reference to Integrated Pest Management (IPM) resources available to Oregon growers. It highlights university, federal, state, and private services and resources that provide support to growers in the implementation of IPM programs. The Guide consists of five sections: • An introduction to the National...
As the dormant season progresses towards the beginning of the 2010 vineyard season, we prepared this newsletter to focus on viticulture topics in disease management and cover cropping.
With pruning taking place across vineyards in Oregon, Dr. Jay Pscheidt reminds vineyard managers to be on alert for canker diseases. Also,...
Root rot of sweet corn in western Oregon and Washington is a significant disease that
can reduce yield of intolerant cultivars of processed sweet corn by fifty percent. Root rot
is caused by a complex of soilborne organisms, including Drechslera sp., Phoma
terrestris, and Pythium arrhenomanes. Processors have adopted tolerant...
From 1996 through 1998 I collected mature female black rockfish off Oregon and examined ovaries and otoliths to estimate weekly, age-specific larval production. In all reproductive seasons, older black rockfish extruded larvae earlier in the season, followed by progressively younger fish. Each year, beginning in May, I collected young-of-the-year (YOY)...
Gall-associated phytopathogens have unique evolutionary histories that haveshaped both their modes of infection and genomic structures. Pathogenicity of the gall-associated plant pathogens of the Rhodococcus, Agrobacterium, and Rathayibactergenera is mediated by horizontally acquired virulence loci. The relative ease of gainand loss of the virulence loci has confounded accurate characterization of...
Modern technology has enabled the advancement of biological research through the use of powerful machines and computers as well as innovative computer programs. Advances in sequencing technology and software enable us to make de novo assemblies of organism genomes, and the development of specialized computer programs can automate routine but...
Protein adsorption on blood contacting medical devices usually causes fouling and failure, and may promote clotting and undesirable immune responses. Hydrophilic polymer coatings in a dense, pendent “brush” conformation can resist protein adsorption on the surface while increasing biocompatibility. Such coatings can also be used to immobilize a variety of...
Focus is published by Oregon State University's College of Forestry. Our goal is to keep Forestry alumni, friends, faculty, staff, and students informed about the College of Forestry and its many activities and programs.
Welcome to the spring edition of the OWRI Technical Newsletter. This edition showcases several articles and resources that will help you to prepare for the 2013 season. Dr. James Osborne, OSU Enology Extension Specialist, opens this edition with a timely article about Brettanomyces and winemaking. You will also find an...
Diseases can be a substantial threat to woody plant production, a major industry in Oregon. Boxwood (Buxus spp.) and rhododendron (Rhododendron subgenus Rhododendron and Hymenanthes) represent the top two broadleaf evergreen woody plants by farmgate value in Oregon. Historically, Phytophthora root rot has been considered the most destructive disease of...
Population genetic structure is widespread in many organisms and can be found at small spatial scales. Fine-scale differentiation is the result of ecological and evolutionary processes working together to produce an overall pattern, but the relative importance of these factors in population differentiation is poorly understood. The goals of my...
Bacterial blight (Xanthomonas arboricola pv. corylina) (Xac) of hazelnut (Corylus avellana L.) was described first in Oregon in 1915 and is now recognized as a damaging disease of young hazelnut trees worldwide. Stressed hazelnut trees in conditions such as planting on marginal sites, and trees between 1 and 4-years-old are...
Plant pathogenic Rhodococcus species are persistent pathogens able to cause severe growth deformities on a large range of hosts. The most well studied species, R. fascians is predicted by current models to synthesize a mixture of cytokinins that act to directly perturb the hosts' hormone balances, which results in abnormal...
Xanthohumol, a prenylated flavonoid found in hops and beer, possesses a long-standing reputation to improve health. Xanthohumol’s cytotoxicity to a wide variety of cancer cell lines is well-established. Herein we provide further insight into the cytotoxicity of not only xanthohumol, but also its non-estrogenic derivatives, to a number of colonic...
The fungus Nothophaeocryptopus gaeumannii is the causative agent of Swiss needle cast (SNC), a foliar disease of Douglas-fir. Disease is characterized by premature loss of foliage and reduced growth resulting from the inhibition of photosynthesis due to the occlusion of stomata by the ascocarps of N. gaeumannii. Although the disease...
This document contains a summary of changes in administrations and actions taken by the Pacific Marine Fisheries Commission in 1974, as well as an update for the status of various fisheries.
Published June 2001. Facts and recommendations in this publication may no longer be valid. Please look for up-to-date information in the OSU Extension Catalog: http://extension.oregonstate.edu/catalog